CONS_ARATH
ID CONS_ARATH Reviewed; 373 AA.
AC Q39057; Q2V373;
DT 05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Zinc finger protein CONSTANS {ECO:0000303|PubMed:7697715};
GN Name=CO {ECO:0000303|PubMed:7697715};
GN OrderedLocusNames=At5g15840 {ECO:0000312|Araport:AT5G15840};
GN ORFNames=F14F8_220 {ECO:0000312|EMBL:CAC01783.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS OF ARG-59 AND
RP 96-LEU--ARG-98, AND MUTANTS CO-1 AND CO-2.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=7697715; DOI=10.1016/0092-8674(95)90288-0;
RA Putterill J.J., Robson F., Lee K., Simon R., Coupland G.;
RT "The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein
RT showing similarities to zinc finger transcription factors.";
RL Cell 80:847-857(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=10834834; DOI=10.1126/science.288.5471.1613;
RA Samach A., Onouchi H., Gold S.E., Ditta G.S., Schwarz-Sommer Z.,
RA Yanofsky M.F., Coupland G.;
RT "Distinct roles of CONSTANS target genes in reproductive development of
RT Arabidopsis.";
RL Science 288:1613-1616(2000).
RN [7]
RP FUNCTION.
RX PubMed=11323677; DOI=10.1038/35074138;
RA Suarez-Lopez P., Wheatley K., Robson F., Onouchi H., Valverde F.,
RA Coupland G.;
RT "CONSTANS mediates between the circadian clock and the control of flowering
RT in Arabidopsis.";
RL Nature 410:1116-1120(2001).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12692345; DOI=10.1104/pp.102.016188;
RA Griffiths S., Dunford R.P., Coupland G., Laurie D.A.;
RT "The evolution of CONSTANS-like gene families in barley, rice, and
RT Arabidopsis.";
RL Plant Physiol. 131:1855-1867(2003).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=15229176; DOI=10.1242/dev.01231;
RA An H., Roussot C., Suarez-Lopez P., Corbesier L., Vincent C., Pineiro M.,
RA Hepworth S., Mouradov A., Justin S., Turnbull C., Coupland G.;
RT "CONSTANS acts in the phloem to regulate a systemic signal that induces
RT photoperiodic flowering of Arabidopsis.";
RL Development 131:3615-3626(2004).
RN [10]
RP INTERACTION WITH SPA1; SPA2; SPA3 AND SPA4, AND MUTAGENESIS OF
RP 214-VAL-PRO-215; 265-VAL-PRO-266 AND 370-VAL-PRO-371.
RX PubMed=16854975; DOI=10.1242/dev.02481;
RA Laubinger S., Marchal V., Le Gourrierec J., Wenkel S., Adrian J., Jang S.,
RA Kulajta C., Braun H., Coupland G., Hoecker U.;
RT "Arabidopsis SPA proteins regulate photoperiodic flowering and interact
RT with the floral inducer CONSTANS to regulate its stability.";
RL Development 133:3213-3222(2006).
RN [11]
RP FUNCTION, AND INTERACTION WITH AS1.
RX PubMed=21950734; DOI=10.1111/j.1365-313x.2011.04793.x;
RA Song Y.H., Lee I., Lee S.Y., Imaizumi T., Hong J.C.;
RT "CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of
RT FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.";
RL Plant J. 69:332-342(2012).
RN [12]
RP INTERACTION WITH ADO3.
RX PubMed=22628657; DOI=10.1126/science.1219644;
RA Song Y.H., Smith R.W., To B.J., Millar A.J., Imaizumi T.;
RT "FKF1 conveys timing information for CONSTANS stabilization in
RT photoperiodic flowering.";
RL Science 336:1045-1049(2012).
RN [13]
RP INTERACTION WITH PHL, AND SUBCELLULAR LOCATION.
RX PubMed=24127609; DOI=10.1073/pnas.1310631110;
RA Endo M., Tanigawa Y., Murakami T., Araki T., Nagatani A.;
RT "PHYTOCHROME-DEPENDENT LATE-FLOWERING accelerates flowering through
RT physical interactions with phytochrome B and CONSTANS.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:18017-18022(2013).
RN [14]
RP INTERACTION WITH NFYC9.
RX PubMed=25105952; DOI=10.1038/ncomms5601;
RA Hou X., Zhou J., Liu C., Liu L., Shen L., Yu H.;
RT "Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus
RT orchestrates flowering responses of Arabidopsis.";
RL Nat. Commun. 5:4601-4601(2014).
RN [15]
RP INTERACTION WITH MRG1 AND MRG2.
RX PubMed=25211338; DOI=10.1371/journal.pgen.1004617;
RA Bu Z., Yu Y., Li Z., Liu Y., Jiang W., Huang Y., Dong A.W.;
RT "Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via
RT interaction with CONSTANS to modulate FT expression.";
RL PLoS Genet. 10:E1004617-E1004617(2014).
RN [16]
RP FUNCTION.
RX PubMed=27255839; DOI=10.1038/nplants.2016.75;
RA Zhu Y., Liu L., Shen L., Yu H.;
RT "NaKR1 regulates long-distance movement of FLOWERING LOCUS T in
RT Arabidopsis.";
RL Nat. Plants 2:16075-16075(2016).
RN [17]
RP INTERACTION WITH MIP1A, AND SUBCELLULAR LOCATION.
RX PubMed=27015278; DOI=10.1371/journal.pgen.1005959;
RA Graeff M., Straub D., Eguen T., Dolde U., Rodrigues V., Brandt R.,
RA Wenkel S.;
RT "Microprotein-mediated recruitment of CONSTANS into a TOPLESS trimeric
RT complex represses flowering in Arabidopsis.";
RL PLoS Genet. 12:E1005959-E1005959(2016).
RN [18]
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 290-357.
RX PubMed=33693873; DOI=10.1093/plcell/koab016;
RA Lv X., Zeng X., Hu H., Chen L., Zhang F., Liu R., Liu Y., Zhou X., Wang C.,
RA Wu Z., Kim C., He Y., Du J.;
RT "Structural insights into the multivalent binding of the Arabidopsis
RT FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor
RT complex.";
RL Plant Cell 33:1182-1195(2021).
CC -!- FUNCTION: Transcription factor that acts in the long day flowering
CC pathway and may mediate between the circadian clock and the control of
CC flowering. Plays a role in the regulation of flowering time by acting
CC on 'SUPPRESSOR OF OVEREXPRESSION OF CO1', 'TERMINAL FLOWER 1' and
CC 'FLOWERING LOCUS T'. Also regulates P5CS2 and ACS10 (involved in
CC proline and ethylene biosynthesis, respectively). Regulates the
CC expression of NAKR1 by binding to the 5'-TGTG(N2-3)ATG-3' motif
CC (PubMed:27255839). {ECO:0000269|PubMed:10834834,
CC ECO:0000269|PubMed:11323677, ECO:0000269|PubMed:21950734,
CC ECO:0000269|PubMed:27255839}.
CC -!- SUBUNIT: Interacts with ADO3, SPA1, SPA2, SPA3 and SPA4
CC (PubMed:16854975, PubMed:22628657). Interacts with MRG1 and MRG2 (via
CC MRG domain) (PubMed:25211338). Interacts (via B-box) with MIP1A
CC (PubMed:27015278). Interacts with AS1 to form a functional complex
CC regulating FT expression (PubMed:21950734). Interacts with NFYC9
CC (PubMed:25105952). Component of a red light-dependent nuclear complex
CC made of PHL, PHYB and CO. Interacts directly with PHL in the presence
CC of PHYB (PubMed:24127609). {ECO:0000269|PubMed:16854975,
CC ECO:0000269|PubMed:21950734, ECO:0000269|PubMed:22628657,
CC ECO:0000269|PubMed:24127609, ECO:0000269|PubMed:25105952,
CC ECO:0000269|PubMed:25211338, ECO:0000269|PubMed:27015278}.
CC -!- INTERACTION:
CC Q39057; O82617: BBX23; NbExp=4; IntAct=EBI-1639724, EBI-15191793;
CC Q39057; Q6NLH4: BBX29; NbExp=4; IntAct=EBI-1639724, EBI-15192709;
CC Q39057; Q9LJB7: BBX32; NbExp=3; IntAct=EBI-1639724, EBI-4425264;
CC Q39057; P43254: COP1; NbExp=8; IntAct=EBI-1639724, EBI-301649;
CC Q39057; Q9LQT8: GAI; NbExp=4; IntAct=EBI-1639724, EBI-963606;
CC Q39057; Q9SLG0: NFYB1; NbExp=6; IntAct=EBI-1639724, EBI-2126009;
CC Q39057; Q9SMP0: NFYC1; NbExp=8; IntAct=EBI-1639724, EBI-2125944;
CC Q39057; Q8L4B2: NFYC9; NbExp=3; IntAct=EBI-1639724, EBI-2466050;
CC Q39057; Q9SLH3: RGA; NbExp=3; IntAct=EBI-1639724, EBI-963624;
CC Q39057; Q39162: TGA4; NbExp=3; IntAct=EBI-1639724, EBI-541600;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24127609,
CC ECO:0000269|PubMed:27015278}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q39057-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q39057-2; Sequence=VSP_036312, VSP_036313;
CC -!- TISSUE SPECIFICITY: Expressed in leaves, shoots and shoot apical
CC meristem. Detected in the vascular tissue of the hypocotyl, the
CC cotyledons and the leaves. Restricted to the protoxylem and phloem in
CC young inflorescence stems and to the phloem only in older
CC inflorescences. Also detected in the vascular tissue of the root.
CC {ECO:0000269|PubMed:15229176}.
CC -!- INDUCTION: Expressed with a circadian rhythm showing a broad peak
CC between 12 hours and dawn. Higher expression under long days.
CC -!- DOMAIN: The CCT domain is essential for the interaction with SPA1.
CC -!- MISCELLANEOUS: The GIGANTEA-CONSTANS-FLOWER LOCUS T (GI-CO-FT) pathway
CC to control photoperiodic flowering under LD is conserved between
CC Arabidopsis and rice, but the regulation of the downstream gene by the
CC upstream regulatory gene is reversed in the two species. In
CC Arabidopsis, GI acts as an activator of CO, which in turn activates the
CC floral activator FT under LD conditions. In rice, GI activates HD1/CO
CC in a similar manner to that in Arabidopsis. However, under LD
CC conditions, HD1 suppresses HD3A/FT expression, causing the suppression
CC of flowering.
CC -!- SIMILARITY: Belongs to the CONSTANS family. {ECO:0000305}.
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DR EMBL; X94937; CAA64407.1; -; mRNA.
DR EMBL; AL391144; CAC01783.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92213.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92214.1; -; Genomic_DNA.
DR EMBL; BT001926; AAN71925.1; -; mRNA.
DR EMBL; AY086574; AAM63636.1; -; mRNA.
DR PIR; A56133; A56133.
DR RefSeq; NP_001031887.1; NM_001036810.2. [Q39057-2]
DR RefSeq; NP_197088.1; NM_121589.2. [Q39057-1]
DR PDB; 7CVO; X-ray; 2.60 A; A/F=290-357.
DR PDB; 7CVQ; X-ray; 3.30 A; A/F/K/P=290-357.
DR PDB; 7VSQ; X-ray; 2.70 A; A/B/C=10-110.
DR PDB; 7WSJ; X-ray; 2.40 A; A/B/C=2-110.
DR PDBsum; 7CVO; -.
DR PDBsum; 7CVQ; -.
DR PDBsum; 7VSQ; -.
DR PDBsum; 7WSJ; -.
DR AlphaFoldDB; Q39057; -.
DR SMR; Q39057; -.
DR BioGRID; 16717; 112.
DR IntAct; Q39057; 97.
DR MINT; Q39057; -.
DR STRING; 3702.AT5G15840.1; -.
DR PaxDb; Q39057; -.
DR PRIDE; Q39057; -.
DR EnsemblPlants; AT5G15840.1; AT5G15840.1; AT5G15840. [Q39057-1]
DR EnsemblPlants; AT5G15840.2; AT5G15840.2; AT5G15840. [Q39057-2]
DR GeneID; 831441; -.
DR Gramene; AT5G15840.1; AT5G15840.1; AT5G15840. [Q39057-1]
DR Gramene; AT5G15840.2; AT5G15840.2; AT5G15840. [Q39057-2]
DR KEGG; ath:AT5G15840; -.
DR Araport; AT5G15840; -.
DR TAIR; locus:2143206; AT5G15840.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_028225_3_2_1; -.
DR InParanoid; Q39057; -.
DR OMA; YRMQEQH; -.
DR PhylomeDB; Q39057; -.
DR PRO; PR:Q39057; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q39057; baseline and differential.
DR Genevisible; Q39057; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IPI:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0010018; P:far-red light signaling pathway; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:0010218; P:response to far red light; IMP:TAIR.
DR InterPro; IPR010402; CCT_domain.
DR InterPro; IPR045281; CONSTANS-like.
DR InterPro; IPR000315; Znf_B-box.
DR PANTHER; PTHR31319; PTHR31319; 1.
DR Pfam; PF06203; CCT; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR SMART; SM00336; BBOX; 2.
DR PROSITE; PS51017; CCT; 1.
DR PROSITE; PS50119; ZF_BBOX; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; Differentiation;
KW DNA-binding; Flowering; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..373
FT /note="Zinc finger protein CONSTANS"
FT /id="PRO_0000113277"
FT DOMAIN 306..348
FT /note="CCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00357"
FT ZN_FING 15..57
FT /note="B box-type 1; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT ZN_FING 58..108
FT /note="B box-type 2; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT REGION 109..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 20
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 23
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 43
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 48
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 63
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 66
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 86
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 91
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT VAR_SEQ 255..274
FT /note="AYISSMETGVVPESTACVTT -> VRLLYICYPFNLASSHNAAG (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036312"
FT VAR_SEQ 275..373
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036313"
FT MUTAGEN 59
FT /note="R->H: In co-2; late-flowering under long day
FT condition."
FT /evidence="ECO:0000269|PubMed:7697715"
FT MUTAGEN 96..98
FT /note="Missing: In co-1; late-flowering under long day
FT condition."
FT /evidence="ECO:0000269|PubMed:7697715"
FT MUTAGEN 214..215
FT /note="VP->AA: In COmVP1-3; no effect on binding to SPA1;
FT when associated with 265-A-A-266 and 370-A-A-371."
FT /evidence="ECO:0000269|PubMed:16854975"
FT MUTAGEN 265..266
FT /note="VP->AA: In COmVP1-3; no effect on binding to SPA1;
FT when associated with 214-A-A-215 and 370-A-A-371."
FT /evidence="ECO:0000269|PubMed:16854975"
FT MUTAGEN 370..371
FT /note="VP->AA: In COmVP1-3; no effect on binding to SPA1;
FT when associated with 214-A-A-215 and 265-A-A-266."
FT /evidence="ECO:0000269|PubMed:16854975"
FT HELIX 303..316
FT /evidence="ECO:0007829|PDB:7CVO"
FT HELIX 317..319
FT /evidence="ECO:0007829|PDB:7CVO"
FT HELIX 329..335
FT /evidence="ECO:0007829|PDB:7CVO"
SQ SEQUENCE 373 AA; 41986 MW; 56C84CF9CD45E4A6 CRC64;
MLKQESNDIG SGENNRARPC DTCRSNACTV YCHADSAYLC MSCDAQVHSA NRVASRHKRV
RVCESCERAP AAFLCEADDA SLCTACDSEV HSANPLARRH QRVPILPISG NSFSSMTTTH
HQSEKTMTDP EKRLVVDQEE GEEGDKDAKE VASWLFPNSD KNNNNQNNGL LFSDEYLNLV
DYNSSMDYKF TGEYSQHQQN CSVPQTSYGG DRVVPLKLEE SRGHQCHNQQ NFQFNIKYGS
SGTHYNDNGS INHNAYISSM ETGVVPESTA CVTTASHPRT PKGTVEQQPD PASQMITVTQ
LSPMDREARV LRYREKRKTR KFEKTIRYAS RKAYAEIRPR VNGRFAKREI EAEEQGFNTM
LMYNTGYGIV PSF
