ID   COPA1_ORYSJ             Reviewed;        1218 AA.
AC   Q9AUR8; B7EQ85; Q10E15;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Coatomer subunit alpha-1;
DE   AltName: Full=Alpha-coat protein 1;
DE            Short=Alpha-COP 1;
GN   OrderedLocusNames=Os03g0711400, LOC_Os03g50340;
GN   ORFNames=OsJ_011822, OSJNBb0033N16.10;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC       dilysine motifs and reversibly associates with Golgi non-clathrin-
CC       coated vesicles, which further mediate biosynthetic protein transport
CC       from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC       complex is required for budding from Golgi membranes, and is essential
CC       for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC       beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Cytoplasmic vesicle, COPI-coated vesicle membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=The coatomer is cytoplasmic or polymerized on
CC       the cytoplasmic side of the Golgi, as well as on the vesicles/buds
CC       originating from it. {ECO:0000250}.
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DR   EMBL; AC082645; AAK18834.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98513.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF12970.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS86024.1; -; Genomic_DNA.
DR   EMBL; CM000140; EAZ28339.1; -; Genomic_DNA.
DR   EMBL; AK100286; BAG94532.1; -; mRNA.
DR   RefSeq; XP_015631030.1; XM_015775544.1.
DR   AlphaFoldDB; Q9AUR8; -.
DR   SMR; Q9AUR8; -.
DR   STRING; 4530.OS03T0711400-01; -.
DR   PaxDb; Q9AUR8; -.
DR   PRIDE; Q9AUR8; -.
DR   EnsemblPlants; Os03t0711400-01; Os03t0711400-01; Os03g0711400.
DR   GeneID; 4333885; -.
DR   Gramene; Os03t0711400-01; Os03t0711400-01; Os03g0711400.
DR   KEGG; osa:4333885; -.
DR   eggNOG; KOG0292; Eukaryota.
DR   HOGENOM; CLU_007565_1_0_1; -.
DR   InParanoid; Q9AUR8; -.
DR   OMA; NWPMLQV; -.
DR   OrthoDB; 139008at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; Q9AUR8; baseline and differential.
DR   Genevisible; Q9AUR8; OS.
DR   GO; GO:0030126; C:COPI vesicle coat; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR016391; Coatomer_asu.
DR   InterPro; IPR010714; Coatomer_asu_C.
DR   InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF04053; Coatomer_WDAD; 1.
DR   Pfam; PF06957; COPI_C; 1.
DR   Pfam; PF00400; WD40; 5.
DR   PIRSF; PIRSF003354; Coatomer_alpha_subunit; 1.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus;
KW   Membrane; Protein transport; Reference proteome; Repeat; Transport;
KW   WD repeat.
FT   CHAIN           1..1218
FT                   /note="Coatomer subunit alpha-1"
FT                   /id="PRO_0000285601"
FT   REPEAT          7..48
FT                   /note="WD 1"
FT   REPEAT          49..88
FT                   /note="WD 2"
FT   REPEAT          91..132
FT                   /note="WD 3"
FT   REPEAT          133..172
FT                   /note="WD 4"
FT   REPEAT          202..241
FT                   /note="WD 5"
FT   REPEAT          246..285
FT                   /note="WD 6"
FT   REPEAT          288..326
FT                   /note="WD 7"
FT   REPEAT          363..404
FT                   /note="WD 8"
FT   REPEAT          450..489
FT                   /note="WD 9"
FT   REGION          857..882
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        864..882
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1218 AA;  136381 MW;  D8B06A8E0F14EB9D CRC64;
     MLTKFETKSN RVKGLSFHPR RPWILASLHS GVIQMWDYRM GTLLDRFDEH DGPVRGVHFH
     ATQPLFVSGG DDYKIKVWNY KTHRCLFTLH GHLDYIRTVQ FHHEYPWIVS ASDDQTIRIW
     NWQSRTCVAV LTGHNHYVMC ASFHPKEDLV VSASLDQTVR VWDIGALRKK TVSPADDILR
     LTQMNTDLFG GVDAVVKYVL EGHDRGVNWA SFHPTLPLIV SGADDRQVKL WRMNDTKAWE
     VDTLRGHMNN VSCVMFHAKQ DIIVSNSEDK SIRIWDATKR TGIQTFRREH DRFWILSAHP
     EMNLLAAGHD SGMIVFKLER ERPAFSVSGD TVFYVKDRFL RFFEFTTQKE VQLAPIRRPG
     SVSLNQSPKT LSYSPTENAV LICSDVDGGS YELYIVPKDS AGRADYLQDA KKGAGGSAVF
     VARNRFAVLE KSSNQVLVKN LKNEIVKKSP LPIATDAIYY AGTGNLLCKA EDRVTIFDLQ
     QRLILGELQA PSVKYVVWSS DMESVALLSK HAVVIANKKL VHRCTLHETI RVKSGAWDEN
     GVFIYTTLNH IKYCLPNGDS GIIKTLDVPI YITRVIGNNI FCLDRDGKNK LVTVDASEYI
     FKLALLRKRY DHVMSMIKNS QLCGQAVISY LQQKGFPEVA LHFVKDEKTR FNLALESGNI
     QIAVASAKEI DDKDHWYRLG IEALRQGNVG IVEYAYQRTK NFERLAFLYL ITGYMDKVGF
     MCKIAGQNNN LMGQFHNALY LGDALKRVEI LENAGQLPLA YITATTHGLT EIADRLAAEL
     GENIPSLPEG KARSLLIPPA PLTASGDWPL LRVMHGIFEG GLDATGKAEL EEDDEAAGAD
     WGDEDLDMVD ASEAMANGGD GFDAEEGEAN EEDGEEGGWD LEDLELPPEA ETPKNAGNAR
     SAVFVAPPPG MPVSLIWTQK SSLAGEHAAA GNFDTAMRLL SRQLGIKNFA PLKPLFVDLH
     MGSHSYLRAL ATAPIIPIAV EKGWSESASP NVRGPPALVF TFPQMEDRLK AAYKATTDGK
     FPEALRQFLS ILHTIPLIVV DSRREVDEVK ELIEIVREYV LGLRMELKRK ELRDDVNRQQ
     ELAAYFTNCK LQRVHMRLVL GSAMGLCYKQ KNFATAEHFA RMLLENNPNE AQARRARQVQ
     QQCSGKKDSS ELNYDYRNPF VVCGATYVPI YRGQKDVSCP YCGSRFVPSI EGQLCTICEL
     AVVGADASGL LCSPTQSR