COPA2_ARATH
ID COPA2_ARATH Reviewed; 1218 AA.
AC Q9SJT9;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Coatomer subunit alpha-2;
DE AltName: Full=Alpha-coat protein 2;
DE Short=Alpha-COP 2;
GN OrderedLocusNames=At2g21390; ORFNames=F3K23.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC dilysine motifs and reversibly associates with Golgi non-clathrin-
CC coated vesicles, which further mediate biosynthetic protein transport
CC from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC complex is required for budding from Golgi membranes, and is essential
CC for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Cytoplasmic vesicle, COPI-coated vesicle membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=The coatomer is cytoplasmic or polymerized on
CC the cytoplasmic side of the Golgi, as well as on the vesicles/buds
CC originating from it. {ECO:0000250}.
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DR EMBL; AC006841; AAD23699.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07173.1; -; Genomic_DNA.
DR EMBL; AK228692; BAF00596.1; -; mRNA.
DR PIR; F84600; F84600.
DR RefSeq; NP_179734.1; NM_127711.4.
DR AlphaFoldDB; Q9SJT9; -.
DR SMR; Q9SJT9; -.
DR BioGRID; 2031; 72.
DR STRING; 3702.AT2G21390.1; -.
DR iPTMnet; Q9SJT9; -.
DR PaxDb; Q9SJT9; -.
DR PRIDE; Q9SJT9; -.
DR ProteomicsDB; 220615; -.
DR EnsemblPlants; AT2G21390.1; AT2G21390.1; AT2G21390.
DR GeneID; 816678; -.
DR Gramene; AT2G21390.1; AT2G21390.1; AT2G21390.
DR KEGG; ath:AT2G21390; -.
DR Araport; AT2G21390; -.
DR TAIR; locus:2050064; AT2G21390.
DR eggNOG; KOG0292; Eukaryota.
DR HOGENOM; CLU_007565_1_0_1; -.
DR InParanoid; Q9SJT9; -.
DR OMA; GMENTDI; -.
DR OrthoDB; 139008at2759; -.
DR PhylomeDB; Q9SJT9; -.
DR PRO; PR:Q9SJT9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJT9; baseline and differential.
DR Genevisible; Q9SJT9; AT.
DR GO; GO:0030126; C:COPI vesicle coat; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProt.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR024977; Apc4_WD40_dom.
DR InterPro; IPR016391; Coatomer_asu.
DR InterPro; IPR010714; Coatomer_asu_C.
DR InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR011048; Haem_d1_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF04053; Coatomer_WDAD; 1.
DR Pfam; PF06957; COPI_C; 1.
DR Pfam; PF00400; WD40; 3.
DR PIRSF; PIRSF003354; Coatomer_alpha_subunit; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF51004; SSF51004; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus;
KW Membrane; Protein transport; Reference proteome; Repeat; Transport;
KW WD repeat.
FT CHAIN 1..1218
FT /note="Coatomer subunit alpha-2"
FT /id="PRO_0000285600"
FT REPEAT 7..48
FT /note="WD 1"
FT REPEAT 49..88
FT /note="WD 2"
FT REPEAT 91..132
FT /note="WD 3"
FT REPEAT 133..172
FT /note="WD 4"
FT REPEAT 202..241
FT /note="WD 5"
FT REPEAT 246..285
FT /note="WD 6"
FT REPEAT 288..326
FT /note="WD 7"
FT REPEAT 363..404
FT /note="WD 8"
FT REGION 826..849
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1218 AA; 136467 MW; AEDBF964C904F267 CRC64;
MLTKFETKSN RVKGLSFHPK RPWILASLHS GVIQLWDYRM GTLIDRFDEH EGPVRGVHFH
NSQPLFVSGG DDYKIKVWNY KTHRCLFTLL GHLDYIRTVQ FHHENPWIVS ASDDQTIRIW
NWQSRTCISV LTGHNHYVMC ASFHPKEDLV VSASLDQTVR VWDIGALKKK SASPADDLMR
FSQMNSDLFG GVDAIVKYVL EGHDRGVNWA SFHPTLPLIV SGADDRQVKL WRMNETKAWE
VDTLRGHMNN VSSVMFHAKQ DIIVSNSEDK SIRVWDATKR TGIQTFRREH DRFWILAVHP
EINLLAAGHD NGMIVFKLER ERPAFALSGD SLFYAKDRFL RYYEYSTQKD SQVIPIRRPG
TPSLNQSPRT LSYSPTENAV LICSDLDGGS YELYIIPKDS VGRSDVVQDA KRGTGGSAVF
IARNRFAVLE KSTSQVLVKN LKNEVVKKSS LPIPTDAIFY AGTGNLLCRS EDKVVIFDLQ
QRLVLGELQT PFVRYVVWSN DMESVALLSK HTIIIASKKL VLQCTLHETI RVKSGAWDDN
GVFIYTTLNH IKYCLPNGDS GIIRTLDVPI YITKVSGNTI FCLDRDGKNR AITINATEYI
FKLALLRKKY DHVMSMIKNS QLCGQAMIAY LQQKGFPEVA LHFVEDERIR FNLALESGNI
SVAVASATEI NEKDHWYRLG VEALRQGNSR IVEFAYQQTK NFERLSFLYL ITGNLDKLSK
LMKIAEVKNN VMGQFHNALY LGDVKERVKI LENAGHLPLA YITASVHGLT DIAERLAIEL
GDNVPSLPEG KTPSLLMPPS PIMCGGDWPL LRVMKGIFEG GLESANRGAV DEEEEDVEGD
WGEGLDKFDV DGMENTDIEA ILDGAEAGEE EDDEEGGWGL DLDLPPELDT PKASANARSS
TFVTPPQGMP VSQIWSQKSS LAAEQAAAGS FDTAMRLLHR QLGIKNFAPL KSMFLDLFSG
SHSYLRAFSS SPVVPLAIER GWSESSSPNV RGPPALVFDF SQLEAKLKSG YKATTAGKLS
EALRVFLSIL QTIPLVVVES RREVDEVKEL VIIVKEYVLG LQLELKRREM KDDPVRQQEL
AAYFTHCKLQ TPHLRLAYFS AMTVCYKSKN MATAAHFARS LLDTNPTIES QARTARQVMQ
AAERNMTDAT TLNYDFRNPF VICGSTYVPI YKGQKDVACP YCTARFVPSQ EGNICSVCDL
AVIGADASGL LCSASQVR
