ACPM_MYCS2
ID ACPM_MYCS2 Reviewed; 99 AA.
AC A0R0B3; I7G4X4;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Meromycolate extension acyl carrier protein;
DE Short=ACP;
GN Name=acpM; OrderedLocusNames=MSMEG_4326, MSMEI_4226;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP PUPYLATION AT LYS-79, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=20094657; DOI=10.1039/b916104j;
RA Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA Barry C.E. III, Bark S., Dorrestein P.C.;
RT "Expansion of the mycobacterial 'PUPylome'.";
RL Mol. Biosyst. 6:376-385(2010).
CC -!- FUNCTION: Acyl carrier protein involved in meromycolate extension.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC of apo-AcpM. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the acyl carrier protein (ACP) family.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK73710.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP40682.1; -; Genomic_DNA.
DR RefSeq; WP_003895721.1; NZ_SIJM01000003.1.
DR RefSeq; YP_888601.1; NC_008596.1.
DR PDB; 7BVC; EM; 2.90 A; P=1-99.
DR PDB; 7BVE; EM; 2.81 A; C/D=1-99.
DR PDB; 7BVF; EM; 2.97 A; P=1-99.
DR PDB; 7BVG; EM; 3.10 A; P=1-99.
DR PDB; 7BVH; X-ray; 3.30 A; C/D=1-99.
DR PDB; 7BWR; EM; 3.50 A; C/D=1-99.
DR PDB; 7BX8; EM; 3.60 A; C/D=1-99.
DR PDBsum; 7BVC; -.
DR PDBsum; 7BVE; -.
DR PDBsum; 7BVF; -.
DR PDBsum; 7BVG; -.
DR PDBsum; 7BVH; -.
DR PDBsum; 7BWR; -.
DR PDBsum; 7BX8; -.
DR AlphaFoldDB; A0R0B3; -.
DR SMR; A0R0B3; -.
DR STRING; 246196.MSMEI_4226; -.
DR PRIDE; A0R0B3; -.
DR EnsemblBacteria; ABK73710; ABK73710; MSMEG_4326.
DR EnsemblBacteria; AFP40682; AFP40682; MSMEI_4226.
DR GeneID; 66735671; -.
DR KEGG; msg:MSMEI_4226; -.
DR KEGG; msm:MSMEG_4326; -.
DR PATRIC; fig|246196.19.peg.4244; -.
DR eggNOG; COG0236; Bacteria.
DR OMA; CEIPDEQ; -.
DR OrthoDB; 1943389at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000036; F:acyl carrier activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.1200.10; -; 1.
DR HAMAP; MF_01217; Acyl_carrier; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR003231; Acyl_carrier.
DR InterPro; IPR009081; PP-bd_ACP.
DR PANTHER; PTHR20863; PTHR20863; 1.
DR Pfam; PF00550; PP-binding; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Fatty acid biosynthesis; Fatty acid metabolism;
KW Isopeptide bond; Lipid biosynthesis; Lipid metabolism; Phosphopantetheine;
KW Phosphoprotein; Reference proteome; Ubl conjugation.
FT CHAIN 1..99
FT /note="Meromycolate extension acyl carrier protein"
FT /id="PRO_0000397183"
FT DOMAIN 3..81
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 41
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT CROSSLNK 79
FT /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT with Q-Cter in protein Pup)"
FT /evidence="ECO:0000269|PubMed:20094657"
FT HELIX 5..19
FT /evidence="ECO:0007829|PDB:7BVE"
FT HELIX 24..26
FT /evidence="ECO:0007829|PDB:7BVF"
FT HELIX 33..37
FT /evidence="ECO:0007829|PDB:7BVE"
FT HELIX 41..54
FT /evidence="ECO:0007829|PDB:7BVE"
FT TURN 62..66
FT /evidence="ECO:0007829|PDB:7BVE"
FT HELIX 70..80
FT /evidence="ECO:0007829|PDB:7BVE"
FT HELIX 88..90
FT /evidence="ECO:0007829|PDB:7BVG"
SQ SEQUENCE 99 AA; 10737 MW; 76832E8DB47EFCCD CRC64;
MAATQEEIIA GLAEIIEEVT GIEPSEVTPE KSFVDDLDID SLSMVEIAVQ TEDKYGVKIP
DEDLAGLRTV GDVVAYIQKL EEENPEAAAA LREKFAADQ
