COPA_PSESM
ID COPA_PSESM Reviewed; 589 AA.
AC P59571;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Copper resistance protein A homolog;
DE Flags: Precursor;
GN Name=copA; OrderedLocusNames=PSPTO_3914;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- FUNCTION: Could be involved in copper resistance. May have oxidase
CC activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- PTM: Predicted to be exported by the Tat system. The position of the
CC signal peptide cleavage has not been experimentally proven.
CC -!- SIMILARITY: Belongs to the multicopper oxidase family. CopA subfamily.
CC {ECO:0000305}.
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DR EMBL; AE016853; AAO57380.1; -; Genomic_DNA.
DR RefSeq; NP_793685.1; NC_004578.1.
DR RefSeq; WP_011104780.1; NC_004578.1.
DR AlphaFoldDB; P59571; -.
DR SMR; P59571; -.
DR STRING; 223283.PSPTO_3914; -.
DR EnsemblBacteria; AAO57380; AAO57380; PSPTO_3914.
DR GeneID; 1185587; -.
DR KEGG; pst:PSPTO_3914; -.
DR PATRIC; fig|223283.9.peg.4013; -.
DR eggNOG; COG2132; Bacteria.
DR HOGENOM; CLU_009100_5_2_6; -.
DR OMA; WNQMRMS; -.
DR OrthoDB; 971126at2; -.
DR PhylomeDB; P59571; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR CDD; cd13848; CuRO_1_CopA; 1.
DR CDD; cd13874; CuRO_2_CopA; 1.
DR CDD; cd13896; CuRO_3_CopA; 1.
DR Gene3D; 2.60.40.420; -; 3.
DR InterPro; IPR001117; Cu-oxidase.
DR InterPro; IPR011706; Cu-oxidase_C.
DR InterPro; IPR045087; Cu-oxidase_fam.
DR InterPro; IPR011707; Cu-oxidase_N.
DR InterPro; IPR006376; Cu-R_CopA.
DR InterPro; IPR033138; Cu_oxidase_CS.
DR InterPro; IPR002355; Cu_oxidase_Cu_BS.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR034284; CuRO_1_CopA.
DR InterPro; IPR034282; CuRO_2_CopA.
DR InterPro; IPR034279; CuRO_3_CopA.
DR InterPro; IPR006311; TAT_signal.
DR InterPro; IPR019546; TAT_signal_bac_arc.
DR PANTHER; PTHR11709; PTHR11709; 1.
DR Pfam; PF00394; Cu-oxidase; 1.
DR Pfam; PF07731; Cu-oxidase_2; 1.
DR Pfam; PF07732; Cu-oxidase_3; 1.
DR SUPFAM; SSF49503; SSF49503; 3.
DR TIGRFAMs; TIGR01480; copper_res_A; 1.
DR TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR PROSITE; PS00079; MULTICOPPER_OXIDASE1; 1.
DR PROSITE; PS00080; MULTICOPPER_OXIDASE2; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Copper; Metal-binding; Oxidoreductase; Periplasm; Reference proteome;
KW Repeat; Signal.
FT SIGNAL 1..32
FT /note="Tat-type signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00648"
FT CHAIN 33..589
FT /note="Copper resistance protein A homolog"
FT /id="PRO_0000002948"
FT REPEAT 367..374
FT /note="1"
FT REPEAT 396..403
FT /note="2"
FT REGION 367..403
FT /note="2 X 8 AA tandem repeats of D-H-X-X-M-X-G-M"
FT BINDING 100
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="1"
FT /note="type 2 copper site"
FT /evidence="ECO:0000250"
FT BINDING 102
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="2"
FT /note="type 3 copper site"
FT /evidence="ECO:0000250"
FT BINDING 142
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="2"
FT /note="type 3 copper site"
FT /evidence="ECO:0000250"
FT BINDING 144
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="3"
FT /note="type 3 copper site"
FT /evidence="ECO:0000250"
FT BINDING 522
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="4"
FT /note="type 1 copper site"
FT /evidence="ECO:0000255"
FT BINDING 525
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="1"
FT /note="type 2 copper site"
FT /evidence="ECO:0000255"
FT BINDING 527
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="3"
FT /note="type 3 copper site"
FT /evidence="ECO:0000255"
FT BINDING 570
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="3"
FT /note="type 3 copper site"
FT /evidence="ECO:0000255"
FT BINDING 571
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="4"
FT /note="type 1 copper site"
FT /evidence="ECO:0000255"
FT BINDING 572
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="2"
FT /note="type 3 copper site"
FT /evidence="ECO:0000255"
FT BINDING 576
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="4"
FT /note="type 1 copper site"
FT /evidence="ECO:0000255"
FT BINDING 581
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /ligand_label="4"
FT /note="type 1 copper site"
FT /evidence="ECO:0000255"
SQ SEQUENCE 589 AA; 66123 MW; 84B88332EAD5D003 CRC64;
MPTRTSRRTF VKGLAASSIL SGLGLWRSPA WALPNPGQPD GLSGTEFDLT IGETQVNITG
NARTAMTING GIPGPLLRWR EGDTVTLRVK NRLDETTSIH WHGIILPANM DGVPGLSFDG
IAPDGMYVYR FKVRQHGTYW YHSHSGFQEQ SGVYGPLVID AKEPEPFTYE REHVVMLTDW
ADEDPARVMK KLKKQSDYYN NNKRTVGDFI NDVGEKGWSA TTAERWMWAQ MKMNPTDLAD
VSGATYTYLM NGQAPNMNWT GLFKPGEQIR LRFINGSSMT YFDVRIPGLK MTVVASDGLH
IKPVVVDELR IAVAETFDVI VEPADGAYTL FAQSMDRTGF ARGTLTSRPG MQAEVPPLDP
RPLLSMDDMG MAGMDHGSMN HSAKPAMDGM DHSKMDHDSM PGMDHGTMPM QEAPVMQSHP
DSERNNPLVD MQAMSTSAKL NDPGIGLRDN GRKVLTYADL RSTFEDPDGR EPSRTIELHL
TGHMEKFAWS FDGVKFSDAK PLMLKYGERV RIVLVNDTMM THPIHLHGMW SDLEDENGQF
MVRKHTIDMP PGSRRSYRVT ADALGRWAYH CHMLYHMEMG MFREVRVEE
