ID   COPB1_ECOLI             Reviewed;          85 AA.
AC   P13959;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Replication regulatory protein RepA2;
DE   AltName: Full=Protein CopB;
GN   Name=repA2; Synonyms=copB; OrderedLocusNames=ECOK12F006;
OS   Escherichia coli (strain K12).
OG   Plasmid F, and Plasmid IncFI P307.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3032897; DOI=10.1128/jb.169.5.1836-1846.1987;
RA   Saadi S., Maas W.K., Hill D.F., Bergquist P.L.;
RT   "Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI
RT   plasmids P307 and F, and its relation to the RepA replicon of IncFII
RT   plasmids.";
RL   J. Bacteriol. 169:1836-1846(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63; PLASMID=F;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This protein is involved in the determination of copy number
CC       in gene replication. It binds to the repA promoter thus inhibiting the
CC       synthesis of the mRNA for the initiator protein RepA.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M16167; AAB17110.1; -; Genomic_DNA.
DR   EMBL; M16168; AAA99007.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97876.1; -; Genomic_DNA.
DR   PIR; A26870; QQECAP.
DR   RefSeq; NP_061385.1; NC_002483.1.
DR   RefSeq; WP_000083834.1; NZ_CP014273.1.
DR   AlphaFoldDB; P13959; -.
DR   SMR; P13959; -.
DR   PRIDE; P13959; -.
DR   PATRIC; fig|83333.107.peg.598; -.
DR   PRO; PR:P13959; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR   InterPro; IPR019661; RepA2.
DR   Pfam; PF10723; RepB-RCR_reg; 1.
PE   4: Predicted;
KW   DNA-binding; Plasmid; Plasmid copy control; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..85
FT                   /note="Replication regulatory protein RepA2"
FT                   /id="PRO_0000068290"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..35
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   85 AA;  9604 MW;  523CABF3EAFEDBC3 CRC64;
     MSQTENAVTS SSGNKRAYRK GNPVPARERQ RASLARRSNT HKAFHAVIQA RLKDRLSELA
     DEEGITQAQM LEKLIESELK RRATL