ID   COPG_STRAG              Reviewed;          45 AA.
AC   P13920;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Protein CopG;
DE   AltName: Full=Protein RepA;
GN   Name=copG; Synonyms=repA;
OS   Streptococcus agalactiae.
OG   Plasmid pLS1, and Plasmid pMV158.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pLS1;
RX   PubMed=2438417; DOI=10.1016/0022-2836(86)90026-4;
RA   Lacks S.A., Lopez P., Greenberg B., Espinosa M.;
RT   "Identification and analysis of genes for tetracycline resistance and
RT   replication functions in the broad-host-range plasmid pLS1.";
RL   J. Mol. Biol. 192:753-765(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pMV158;
RX   PubMed=2677995; DOI=10.1093/nar/17.18.7283;
RA   van der Lelie D., Bron S., Venema G., Oskam L.;
RT   "Similarity of minus origins of replication and flanking open reading
RT   frames of plasmids pUB110, pTB913 and pMV158.";
RL   Nucleic Acids Res. 17:7283-7294(1989).
RN   [3]
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   PLASMID=pLS1;
RX   PubMed=2497439; DOI=10.1093/nar/17.7.2405;
RA   del Solar G.H., de la Campa A.G., Perez-Martin J., Choli T., Espinosa M.;
RT   "Purification and characterization of RepA, a protein involved in the copy
RT   number control of plasmid pLS1.";
RL   Nucleic Acids Res. 17:2405-2420(1989).
RN   [4]
RP   FUNCTION.
RX   PubMed=2373704; DOI=10.1016/s0021-9258(19)38382-6;
RA   del Solar G.H., Perez-Martin J., Espinosa M.;
RT   "Plasmid pLS1-encoded RepA protein regulates transcription from repAB
RT   promoter by binding to a DNA sequence containing a 13-base pair symmetric
RT   element.";
RL   J. Biol. Chem. 265:12569-12575(1990).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS).
RC   PLASMID=pMV158;
RX   PubMed=9857196; DOI=10.1093/emboj/17.24.7404;
RA   Gomis-Rueth F.-X., Sola M., Acebo P., Parraga A., Guasch A., Eritja R.,
RA   Gonzalez A., Espinosa M., del Solar G.H., Coll M.;
RT   "The structure of plasmid-encoded transcriptional repressor CopG unliganded
RT   and bound to its operator.";
RL   EMBO J. 17:7404-7415(1998).
CC   -!- FUNCTION: Regulates the plasmid copy number. Binds to the RepAB
CC       promoter thus controlling the synthesis of the plasmid replication
CC       initiator protein RepB. {ECO:0000269|PubMed:2373704,
CC       ECO:0000269|PubMed:2497439}.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the transcriptional regulatory CopG/NikR family.
CC       {ECO:0000305}.
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DR   EMBL; M29725; AAA98165.1; -; Genomic_DNA.
DR   EMBL; X15669; CAA33710.1; -; Genomic_DNA.
DR   PIR; A25599; A25599.
DR   PIR; S05980; S05980.
DR   RefSeq; NP_040420.1; NC_001380.1.
DR   RefSeq; WP_010889903.1; NC_010096.1.
DR   RefSeq; YP_001586271.1; NC_010096.1.
DR   PDB; 1B01; X-ray; 2.56 A; A/B=1-45.
DR   PDB; 1EA4; X-ray; 2.95 A; A/B/D/E/F/G/H/J/K/L=1-45.
DR   PDB; 2CPG; X-ray; 1.60 A; A/B/C=1-44.
DR   PDBsum; 1B01; -.
DR   PDBsum; 1EA4; -.
DR   PDBsum; 2CPG; -.
DR   AlphaFoldDB; P13920; -.
DR   SMR; P13920; -.
DR   EvolutionaryTrace; P13920; -.
DR   CollecTF; EXPREG_00000920; -.
DR   GO; GO:0032993; C:protein-DNA complex; EXP:CollecTF.
DR   GO; GO:0043565; F:sequence-specific DNA binding; EXP:CollecTF.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR002145; CopG.
DR   InterPro; IPR010985; Ribbon_hlx_hlx.
DR   Pfam; PF01402; RHH_1; 1.
DR   SUPFAM; SSF47598; SSF47598; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; DNA-binding; Plasmid;
KW   Plasmid copy control; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..45
FT                   /note="Protein CopG"
FT                   /id="PRO_0000139317"
FT   DNA_BIND        16..36
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         30
FT                   /note="A->E: 5-fold increase in plasmid copy number."
FT   STRAND          2..10
FT                   /evidence="ECO:0007829|PDB:2CPG"
FT   HELIX           11..24
FT                   /evidence="ECO:0007829|PDB:2CPG"
FT   HELIX           28..41
FT                   /evidence="ECO:0007829|PDB:2CPG"
SQ   SEQUENCE   45 AA;  5115 MW;  B7EB0BF2D869747A CRC64;
     MKKRLTITLS ESVLENLEKM AREMGLSKSA MISVALENYK KGQEK