COPT1_ARATH
ID COPT1_ARATH Reviewed; 170 AA.
AC Q39065; Q547P9;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Copper transporter 1;
DE Short=AtCOPT1;
GN Name=COPT1; OrderedLocusNames=At5g59030; ORFNames=K18B18.10, K18B18.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=7499355; DOI=10.1074/jbc.270.47.28479;
RA Kampfenkel K.K.K., Kushnir S., Babiychuk E., Inze D., Vanmontagu M.;
RT "Molecular characterization of a putative Arabidopsis thaliana copper
RT transporter and its yeast homologue.";
RL J. Biol. Chem. 270:28479-28486(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12650623; DOI=10.1023/a:1022345507112;
RA Sancenon V., Puig S., Mira H., Thiele D.J., Penarrubia L.;
RT "Identification of a copper transporter family in Arabidopsis thaliana.";
RL Plant Mol. Biol. 51:577-587(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=14726516; DOI=10.1074/jbc.m313321200;
RA Sancenon V., Puig S., Mateu-Andres I., Dorcey E., Thiele D.J.,
RA Penarrubia L.;
RT "The Arabidopsis copper transporter COPT1 functions in root elongation and
RT pollen development.";
RL J. Biol. Chem. 279:15348-15355(2004).
CC -!- FUNCTION: Copper transporter involved in copper acquisition and
CC transport in leaves. Required for copper homeostasis and normal plant
CC growth and development. {ECO:0000269|PubMed:12650623,
CC ECO:0000269|PubMed:14726516, ECO:0000269|PubMed:7499355}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the root apex, lateral root primordia,
CC embryo, trichomes, guard cells and pollen grains.
CC {ECO:0000269|PubMed:12650623, ECO:0000269|PubMed:14726516}.
CC -!- INDUCTION: Down-regulated by treatment with high concentrations of
CC copper. {ECO:0000269|PubMed:12650623}.
CC -!- DISRUPTION PHENOTYPE: Increased root length and pollen development
CC defects. {ECO:0000269|PubMed:14726516}.
CC -!- MISCELLANEOUS: The increased root length and pollen development defects
CC phenotypes of the mutants are completely and specifically reversed by
CC copper addition.
CC -!- SIMILARITY: Belongs to the copper transporter (Ctr) (TC 1.A.56) family.
CC SLC31A subfamily. {ECO:0000305}.
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DR EMBL; Z49859; CAA90018.1; -; mRNA.
DR EMBL; AB024027; BAB10779.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97132.1; -; Genomic_DNA.
DR EMBL; AY091090; AAM13909.1; -; mRNA.
DR EMBL; AY123038; AAM67571.1; -; mRNA.
DR EMBL; AF466373; AAL74265.1; -; mRNA.
DR EMBL; AK226835; BAE98928.1; -; mRNA.
DR PIR; T48875; T48875.
DR RefSeq; NP_200711.1; NM_125293.4.
DR AlphaFoldDB; Q39065; -.
DR SMR; Q39065; -.
DR BioGRID; 21264; 1.
DR IntAct; Q39065; 1.
DR STRING; 3702.AT5G59030.1; -.
DR TCDB; 1.A.56.1.1; the copper transporter (ctr) family.
DR PaxDb; Q39065; -.
DR PRIDE; Q39065; -.
DR ProteomicsDB; 241169; -.
DR EnsemblPlants; AT5G59030.1; AT5G59030.1; AT5G59030.
DR GeneID; 836020; -.
DR Gramene; AT5G59030.1; AT5G59030.1; AT5G59030.
DR KEGG; ath:AT5G59030; -.
DR Araport; AT5G59030; -.
DR TAIR; locus:2153306; AT5G59030.
DR eggNOG; KOG3386; Eukaryota.
DR HOGENOM; CLU_079690_1_2_1; -.
DR InParanoid; Q39065; -.
DR OMA; MHHKMMM; -.
DR OrthoDB; 1389393at2759; -.
DR PhylomeDB; Q39065; -.
DR PRO; PR:Q39065; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q39065; baseline and differential.
DR Genevisible; Q39065; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005375; F:copper ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0006878; P:cellular copper ion homeostasis; IBA:GO_Central.
DR GO; GO:0006825; P:copper ion transport; IGI:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR InterPro; IPR007274; Cop_transporter.
DR PANTHER; PTHR12483; PTHR12483; 1.
DR Pfam; PF04145; Ctr; 2.
PE 2: Evidence at transcript level;
KW Copper; Copper transport; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..170
FT /note="Copper transporter 1"
FT /id="PRO_0000195045"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 11..13
FT /note="RPS -> PP (in Ref. 1; CAA90018)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 170 AA; 18399 MW; A51664A9C03DD921 CRC64;
MDHDHMHGMP RPSSSSSSSP SSMMNNGSMN EGGGHHHMKM MMHMTFFWGK NTEVLFSGWP
GTSSGMYALC LIFVFFLAVL TEWLAHSSLL RGSTGDSANR AAGLIQTAVY TLRIGLAYLV
MLAVMSFNAG VFLVALAGHA VGFMLFGSQT FRNTSDDRKT NYVPPSGCAC
