COPZ_YEAST
ID COPZ_YEAST Reviewed; 189 AA.
AC P53600; D6W402;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Coatomer subunit zeta;
DE AltName: Full=Zeta-coat protein;
DE Short=Zeta-COP;
GN Name=RET3; OrderedLocusNames=YPL010W; ORFNames=LPA7W, YP8132.03;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP CHARACTERIZATION.
RX PubMed=8617224; DOI=10.1002/j.1460-2075.1996.tb00528.x;
RA Cosson P., Demolliere C., Hennecke S., Duden R., Letourneur F.;
RT "Delta- and zeta-COP, two coatomer subunits homologous to clathrin-
RT associated proteins, are involved in ER retrieval.";
RL EMBO J. 15:1792-1798(1996).
RN [5]
RP FUNCTION.
RX PubMed=9058184; DOI=10.1093/oxfordjournals.jbchem.a021574;
RA Yamazaki S., Harashima S., Sakaguchi M., Mihara K.;
RT "Identification and functional characterization of yeast zeta-COP.";
RL J. Biochem. 121:8-14(1997).
CC -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC dilysine motifs and reversibly associates with Golgi non-clathrin-
CC coated vesicles, which further mediate biosynthetic protein transport
CC from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC complex is required for budding from Golgi membranes, and is essential
CC for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC (PubMed:9058184). The zeta subunit may be involved in regulating the
CC coat assembly and, hence, the rate of biosynthetic protein transport
CC due to its association-dissociation properties with the coatomer
CC complex (PubMed:9058184). {ECO:0000269|PubMed:9058184}.
CC -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC beta', gamma, delta, epsilon and zeta subunits.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Cytoplasmic vesicle, COPI-coated vesicle membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=The coatomer is cytoplasmic or polymerized on
CC the cytoplasmic side of the Golgi, as well as on the vesicles/buds
CC originating from it. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC {ECO:0000305}.
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DR EMBL; U33335; AAB68095.1; -; Genomic_DNA.
DR EMBL; Z71255; CAA95031.1; -; Genomic_DNA.
DR EMBL; Z48483; CAA88376.1; -; Genomic_DNA.
DR EMBL; AY558438; AAS56764.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11418.1; -; Genomic_DNA.
DR PIR; S52521; S52521.
DR RefSeq; NP_015315.1; NM_001183824.1.
DR AlphaFoldDB; P53600; -.
DR SMR; P53600; -.
DR BioGRID; 36167; 231.
DR ComplexPortal; CPX-1652; COPI vesicle coat complex.
DR DIP; DIP-6809N; -.
DR IntAct; P53600; 9.
DR MINT; P53600; -.
DR STRING; 4932.YPL010W; -.
DR iPTMnet; P53600; -.
DR MaxQB; P53600; -.
DR PaxDb; P53600; -.
DR PRIDE; P53600; -.
DR EnsemblFungi; YPL010W_mRNA; YPL010W; YPL010W.
DR GeneID; 856097; -.
DR KEGG; sce:YPL010W; -.
DR SGD; S000005931; RET3.
DR VEuPathDB; FungiDB:YPL010W; -.
DR eggNOG; KOG3343; Eukaryota.
DR GeneTree; ENSGT00390000004405; -.
DR HOGENOM; CLU_086803_0_0_1; -.
DR InParanoid; P53600; -.
DR OMA; NEQTIMS; -.
DR BioCyc; YEAST:G3O-33929-MON; -.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR PRO; PR:P53600; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P53600; protein.
DR GO; GO:0030126; C:COPI vesicle coat; IDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IMP:SGD.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR039652; Coatomer_zeta.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR PANTHER; PTHR11043; PTHR11043; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus;
KW Membrane; Protein transport; Reference proteome; Transport.
FT CHAIN 1..189
FT /note="Coatomer subunit zeta"
FT /id="PRO_0000193830"
SQ SEQUENCE 189 AA; 21688 MW; 685F3024F42B9FA2 CRC64;
MSSLSLYTVQ AVLILDQQGE RIYAKYYQPP HRSDEGHQLL FNSVKKQKEF EKQLYRKTHK
QDSEILIFED HLVLYKEYID ITIYLVASLE ENEIVLQQGF SAIRGALDLI LNSGMDKKNI
QENYDMVLLA IDETIDNGVI LETDSNTIAS RVSKPPTNEP QMALDLDKGF LGAWGFAKSK
FQERLQQGL
