COQ4_YEAST
ID COQ4_YEAST Reviewed; 335 AA.
AC O13525; D6VSI5; Q03454;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Ubiquinone biosynthesis protein COQ4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03111};
DE AltName: Full=Coenzyme Q biosynthesis protein 4 {ECO:0000255|HAMAP-Rule:MF_03111};
DE Flags: Precursor;
GN Name=COQ4 {ECO:0000255|HAMAP-Rule:MF_03111}; OrderedLocusNames=YDR204W;
GN ORFNames=YD8142.01;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP CHARACTERIZATION, MUTAGENESIS OF GLU-226, AND SUBCELLULAR LOCATION.
RX PubMed=11469793; DOI=10.1006/abbi.2001.2448;
RA Belogrudov G.I., Lee P.T., Jonassen T., Hsu A.Y., Gin P., Clarke C.F.;
RT "Yeast COQ4 encodes a mitochondrial protein required for coenzyme Q
RT synthesis.";
RL Arch. Biochem. Biophys. 392:48-58(2001).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP FUNCTION.
RX PubMed=15548532; DOI=10.1074/jbc.m411527200;
RA Gin P., Clarke C.F.;
RT "Genetic evidence for a multi-subunit complex in coenzyme Q biosynthesis in
RT yeast and the role of the Coq1 hexaprenyl diphosphate synthase.";
RL J. Biol. Chem. 280:2676-2681(2005).
RN [6]
RP INTERACTION WITH COQ3.
RX PubMed=15792955; DOI=10.1074/jbc.m501315200;
RA Marbois B.N., Gin P., Faull K.F., Poon W.W., Lee P.T., Strahan J.,
RA Shepherd J.N., Clarke C.F.;
RT "Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the
RT biosynthesis of coenzyme Q.";
RL J. Biol. Chem. 280:20231-20238(2005).
RN [7]
RP SUBUNIT.
RX PubMed=16624818; DOI=10.1074/jbc.m513267200;
RA Tran U.C., Marbois B.N., Gin P., Gulmezian M., Jonassen T., Clarke C.F.;
RT "Complementation of Saccharomyces cerevisiae coq7 mutants by mitochondrial
RT targeting of the Escherichia coli UbiF polypeptide: two functions of yeast
RT Coq7 polypeptide in coenzyme Q biosynthesis.";
RL J. Biol. Chem. 281:16401-16409(2006).
RN [8]
RP FUNCTION, IDENTIFICATION IN COQ ENZYME COMPLEX, AND INTERACTION WITH COQ9.
RX PubMed=17391640; DOI=10.1016/j.abb.2007.02.016;
RA Hsieh E.J., Gin P., Gulmezian M., Tran U.C., Saiki R., Marbois B.N.,
RA Clarke C.F.;
RT "Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the
RT mitochondrial coenzyme Q biosynthetic complex.";
RL Arch. Biochem. Biophys. 463:19-26(2007).
RN [9]
RP FUNCTION, AND MUTAGENESIS OF GLY-120; GLU-121 AND GLU-226.
RX PubMed=19022396; DOI=10.1016/j.bbalip.2008.10.006;
RA Marbois B.N., Gin P., Gulmezian M., Clarke C.F.;
RT "The yeast Coq4 polypeptide organizes a mitochondrial protein complex
RT essential for coenzyme Q biosynthesis.";
RL Biochim. Biophys. Acta 1791:69-75(2009).
CC -!- FUNCTION: Component of the coenzyme Q biosynthetic pathway. May play a
CC role in organizing a multi-subunit COQ enzyme complex required for
CC coenzyme Q biosynthesis. Required for steady-state levels of COQ3,
CC COQ4, COQ6, COQ7 and COQ9 polypeptides. {ECO:0000255|HAMAP-
CC Rule:MF_03111, ECO:0000269|PubMed:15548532,
CC ECO:0000269|PubMed:17391640, ECO:0000269|PubMed:19022396}.
CC -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03111}.
CC -!- SUBUNIT: Component of a multi-subunit COQ enzyme complex, composed of
CC at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9. Interacts with COQ3.
CC {ECO:0000255|HAMAP-Rule:MF_03111, ECO:0000269|PubMed:15792955,
CC ECO:0000269|PubMed:16624818, ECO:0000269|PubMed:17391640}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_03111, ECO:0000269|PubMed:11469793,
CC ECO:0000269|PubMed:14562095}; Peripheral membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_03111, ECO:0000269|PubMed:11469793,
CC ECO:0000269|PubMed:14562095}; Matrix side {ECO:0000255|HAMAP-
CC Rule:MF_03111, ECO:0000269|PubMed:11469793,
CC ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the COQ4 family. {ECO:0000255|HAMAP-
CC Rule:MF_03111}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z68194; CAA92343.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12045.1; -; Genomic_DNA.
DR PIR; S61567; S61567.
DR RefSeq; NP_010490.1; NM_001180512.1.
DR AlphaFoldDB; O13525; -.
DR SMR; O13525; -.
DR BioGRID; 32254; 121.
DR ComplexPortal; CPX-1155; CoQ biosynthetic complex.
DR IntAct; O13525; 20.
DR STRING; 4932.YDR204W; -.
DR MaxQB; O13525; -.
DR PaxDb; O13525; -.
DR PRIDE; O13525; -.
DR DNASU; 851785; -.
DR EnsemblFungi; YDR204W_mRNA; YDR204W; YDR204W.
DR GeneID; 851785; -.
DR KEGG; sce:YDR204W; -.
DR SGD; S000002612; COQ4.
DR VEuPathDB; FungiDB:YDR204W; -.
DR eggNOG; KOG3244; Eukaryota.
DR GeneTree; ENSGT00390000003828; -.
DR HOGENOM; CLU_061241_0_2_1; -.
DR InParanoid; O13525; -.
DR OMA; KFFEFAN; -.
DR BioCyc; YEAST:G3O-29788-MON; -.
DR UniPathway; UPA00232; -.
DR PRO; PR:O13525; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; O13525; protein.
DR GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:WormBase.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; IMP:UniProtKB.
DR HAMAP; MF_03111; Coq4; 1.
DR InterPro; IPR007715; Coq4.
DR InterPro; IPR027540; Coq4_euk.
DR PANTHER; PTHR12922; PTHR12922; 1.
DR Pfam; PF05019; Coq4; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Ubiquinone biosynthesis.
FT TRANSIT 1..10
FT /note="Mitochondrion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03111"
FT CHAIN 11..335
FT /note="Ubiquinone biosynthesis protein COQ4, mitochondrial"
FT /id="PRO_0000006035"
FT MUTAGEN 120
FT /note="G->E: In COQ4-3; abolishes coenzyme Q biosynthesis,
FT but does not affect stability of other COQ polypeptides."
FT /evidence="ECO:0000269|PubMed:19022396"
FT MUTAGEN 121
FT /note="E->K: In COQ4-2; abolishes coenzyme Q biosynthesis,
FT but does not affect stability of other COQ polypeptides."
FT /evidence="ECO:0000269|PubMed:19022396"
FT MUTAGEN 226
FT /note="E->K: In COQ4-1; abolishes coenzyme Q biosynthesis,
FT but does not affect stability of other COQ polypeptides."
FT /evidence="ECO:0000269|PubMed:11469793,
FT ECO:0000269|PubMed:19022396"
SQ SEQUENCE 335 AA; 38627 MW; C8AFBFA249007901 CRC64;
MLRLSLLRST ATLPVKCQRR GLILPAAAMY TLGSLIFGKE ARLADAMERG ELHNKNVDYA
KEAEERTELR IRALANTRPM EPRYNGHVPL HRYEKLLLFA ISGWNSFFHP EDGYNIVQLG
EATALPVFLE NLKQTMLSDS SGRRILKEQP NITTEILHMD KLAKLPHNTF GYVYYQWLKR
ENVSPDTRAP VKFIDDPMHA YIFKRYRQCH DFYHAITNMP IIIEGEITIK ALEGANLGVP
MAILGGILAP LRLKKVQRKR LYNIYLPWAV RTGLSCKPLI NVYWEEMLEK DVTALRKELK
ITLPPDLRTM RKERAALRKE IDAKYNSQKR ATTPA
