ID   2AMD_PSEOL              Reviewed;          54 AA.
AC   P81593;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=2-aminomuconate deaminase;
DE            EC=3.5.99.5;
DE   Flags: Fragment;
OS   Pseudomonas oleovorans.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas;
OC   Pseudomonas oleovorans/pseudoalcaligenes group.
OX   NCBI_TaxID=301;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=JS45;
RX   PubMed=9573204; DOI=10.1128/jb.180.9.2502-2506.1998;
RA   He Z., Spain J.C.;
RT   "A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway
RT   of Pseudomonas pseudoalcaligenes JS45.";
RL   J. Bacteriol. 180:2502-2506(1998).
CC   -!- FUNCTION: Converts 2-aminomuconate to 4-oxalocrotonate, an intermediate
CC       step in the biodegradation of nitrobenzene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-aminomuconate + H2O = (3E)-2-oxohex-3-enedioate +
CC         NH4(+); Xref=Rhea:RHEA:20996, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:64908, ChEBI:CHEBI:77859; EC=3.5.99.5;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=67 uM for 2-aminomuconate;
CC         Vmax=125 umol/min/mg enzyme with glucose as substrate;
CC       pH dependence:
CC         Optimum pH is 6.6.;
CC   -!- PATHWAY: Xenobiotic degradation; nitrobenzene degradation.
CC   -!- SUBUNIT: Homohexamer.
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DR   AlphaFoldDB; P81593; -.
DR   SMR; P81593; -.
DR   BioCyc; MetaCyc:MON-13320; -.
DR   BRENDA; 3.5.99.11; 5150.
DR   UniPathway; UPA00923; -.
DR   GO; GO:0050540; F:2-aminomuconate deaminase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.30.1330.40; -; 1.
DR   InterPro; IPR035959; RutC-like_sf.
DR   SUPFAM; SSF55298; SSF55298; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..>54
FT                   /note="2-aminomuconate deaminase"
FT                   /id="PRO_0000064358"
FT   NON_TER         54
SQ   SEQUENCE   54 AA;  5582 MW;  E4DA7200DA1FCEA3 CRC64;
     STLSSNDAKV VDGKATPLGS FPHVKRAGDF LYVSGTSSRR PDNTIAGAEL DSTS