ID   COR2_PAPSO              Reviewed;         321 AA.
AC   Q9SQ64;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Non-functional NADPH-dependent codeinone reductase 2;
DE            EC=1.1.1.-;
GN   Name=COR2;
OS   Papaver somniferum (Opium poppy).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Papaveraceae; Papaveroideae;
OC   Papaver.
OX   NCBI_TaxID=3469;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 245-248.
RX   PubMed=10417697; DOI=10.1046/j.1365-313x.1999.00470.x;
RA   Unterlinner B., Lenz R., Kutchan T.M.;
RT   "Molecular cloning and functional expression of codeinone reductase: the
RT   penultimate enzyme in morphine biosynthesis in the opium poppy Papaver
RT   somniferum.";
RL   Plant J. 18:465-475(1999).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}. Note=Present in
CC       the cytosolic part of laticifer cells that secrete latex.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
CC   -!- CAUTION: COR2 seems not to have codeinone reductase activity.
CC       {ECO:0000305|PubMed:10417697}.
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DR   EMBL; AF108438; AAF13742.1; -; mRNA.
DR   AlphaFoldDB; Q9SQ64; -.
DR   SMR; Q9SQ64; -.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   CDD; cd19124; AKR_AKR4A_4B; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044497; AKR4A/B.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase.
FT   CHAIN           1..321
FT                   /note="Non-functional NADPH-dependent codeinone reductase
FT                   2"
FT                   /id="PRO_0000418596"
FT   ACT_SITE        56
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         119
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         214..273
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            86
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   321 AA;  35985 MW;  B3943B1EA8513182 CRC64;
     MEIGGVPVVT LSSGRGMPIL GMGTAENNLQ GSERVKLAIL KAIEVGYRHF DTAFVYQTEG
     SLGEAVAEAL QNGLIKSRDE LFITSKLWCA DAYPDHVLPA LQNSLRNLKL EYLDLYLIHW
     PVSLKPGKFV HPIPKDEIFP IDYKSVWAAM EKCQMLGLTK SIGVSNFSCK KLHYLMATAN
     IPPAVNQVEM NPIWQQQKLR DYCKTNNIMV TAYSPLGAKG TMWGSSGVMD SEVLNQISQV
     RGKSVAQVSL RWVYEQGASL LVKSFNEERM KENLKIFDWE LSPEDLKNIS ELPQRRVSTG
     DPFVSINGPF KSVEELWDDE V