ID   2AP1_BRAOT              Reviewed;         256 AA.
AC   Q96356;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Floral homeotic protein APETALA 1-2;
DE            Short=Boi2AP1;
DE   AltName: Full=Agamous-like MADS-box protein 2AP1;
GN   Name=2AP1;
OS   Brassica oleracea var. italica (Broccoli).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=36774;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower;
RX   PubMed=9084216; DOI=10.1007/bf01007702;
RA   Carr S.M., Irish V.F.;
RT   "Floral homeotic gene expression defines developmental arrest stages in
RT   Brassica oleracea L. vars. botrytis and italica.";
RL   Planta 201:179-188(1997).
CC   -!- FUNCTION: Transcription factor that promotes early floral meristem
CC       identity in synergy with LEAFY. Displays a redundant function with
CC       CAULIFLOWER in the up-regulation of LEAFY. Required subsequently for
CC       the transition of an inflorescence meristem into a floral meristem, and
CC       for the normal development of sepals and petals in flowers. Regulates
CC       positively B class homeotic proteins (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U67452; AAB08876.1; -; mRNA.
DR   AlphaFoldDB; Q96356; -.
DR   SMR; Q96356; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002487; TF_Kbox.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF01486; K-box; 1.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS51297; K_BOX; 1.
DR   PROSITE; PS00350; MADS_BOX_1; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Developmental protein; Differentiation;
KW   DNA-binding; Flowering; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..256
FT                   /note="Floral homeotic protein APETALA 1-2"
FT                   /id="PRO_0000417136"
FT   DOMAIN          1..61
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT   DOMAIN          88..178
FT                   /note="K-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT   REGION          187..206
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   256 AA;  30171 MW;  71936E3479B4DB48 CRC64;
     MGRGRVQLKR IENKINRQVT FSKRRAGLMK KAHEISVLCD AEVALVVFSH KGKLFEYSTD
     SCMEKILERY ERYSYAERQL IAPESDSNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM
     SPKELQNLEQ QLDTALKHIR SRKNQLMYDS INELQRKEKA IQEQNSMLSK QIKERENVLR
     AQQEQWDEQN HGHNMPPPPP PQQHQIQHPY MLSHQPSPFL NMGGLYQEED QMAMRRNDLD
     LSLEPVYNCN LGCFAA