CORZ_DROME
ID CORZ_DROME Reviewed; 154 AA.
AC Q26377; Q9VFK7;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Pro-corazonin;
DE Short=Crz;
DE Short=Dm-Crz;
DE Contains:
DE RecName: Full=Corazonin;
DE Contains:
DE RecName: Full=Corazonin precursor-related peptide;
DE Short=CPRP;
DE AltName: Full=Crz-associated peptide;
DE Flags: Precursor;
GN Name=Crz; ORFNames=CG3302;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
RX PubMed=7945373; DOI=10.1006/bbrc.1994.2458;
RA Veenstra J.A.;
RT "Isolation and structure of the Drosophila corazonin gene.";
RL Biochem. Biophys. Res. Commun. 204:292-296(1994).
RN [4]
RP PROTEIN SEQUENCE OF 20-30, PYROGLUTAMATE FORMATION AT GLN-20, AND AMIDATION
RP AT ASN-30.
RC TISSUE=Larva;
RX PubMed=12171930; DOI=10.1074/jbc.m206257200;
RA Baggerman G., Cerstiaens A., De Loof A., Schoofs L.;
RT "Peptidomics of the larval Drosophila melanogaster central nervous
RT system.";
RL J. Biol. Chem. 277:40368-40374(2002).
RN [5]
RP MASS SPECTROMETRY.
RC TISSUE=Cerebral ganglion, and Corpora cardiaca;
RX PubMed=15174081; DOI=10.1002/cne.20145;
RA Predel R., Wegener C., Russell W.K., Tichy S.E., Russell D.H.,
RA Nachman R.J.;
RT "Peptidomics of CNS-associated neurohemal systems of adult Drosophila
RT melanogaster: a mass spectrometric survey of peptides from individual
RT flies.";
RL J. Comp. Neurol. 474:379-392(2004).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Head;
RX PubMed=15669053; DOI=10.1002/cne.20419;
RA Choi Y.J., Lee G., Hall J.C., Park J.H.;
RT "Comparative analysis of Corazonin-encoding genes (Crz's) in Drosophila
RT species and functional insights into Crz-expressing neurons.";
RL J. Comp. Neurol. 482:372-385(2005).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=18087727; DOI=10.1007/s00441-007-0549-5;
RA Lee G., Kim K.-M., Kikuno K., Wang Z., Choi Y.-J., Park J.H.;
RT "Developmental regulation and functions of the expression of the
RT neuropeptide corazonin in Drosophila melanogaster.";
RL Cell Tissue Res. 331:659-673(2008).
CC -!- FUNCTION: Cardioactive peptide. Corazonin is probably involved in the
CC physiological regulation of the heart beat. Clock (Clk) and cycle (cyc)
CC proteins negatively regulate Crz transcription in a cell-specific
CC manner. {ECO:0000269|PubMed:15669053}.
CC -!- SUBCELLULAR LOCATION: [Corazonin]: Secreted.
CC -!- SUBCELLULAR LOCATION: [Corazonin precursor-related peptide]: Secreted
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: From late embryo to larva, expression is
CC consistently detected in three neuronal groups: dorso-lateral neurons
CC (DL), dorso-medial neurons (DM), and neurons in the ventral nerve cord
CC (vCrz). Both the vCrz and DM groups die via programmed cell death
CC during metamorphosis, whereas the DL neurons persist to adulthood. In
CC adults, expression is seen in a cluster of six to eight neurons per
CC lobe in the pars lateralis (DLP), in numerous neuronal cells in the
CC optic lobes, and in a novel group of four abdominal ganglionic neurons
CC present only in males (ms-aCrz). Projections of the ms-aCrz neurons
CC terminate within the ventral nerve cord, implying a role as
CC interneurons. Terminals of the DLP neurons are found in the
CC retrocerebral complex that produces juvenile hormone and adipokinetic
CC hormone, located in the vicinity of terminals emanating from PDF-
CC containing pacemaking neurons. {ECO:0000269|PubMed:15669053,
CC ECO:0000269|PubMed:18087727}.
CC -!- MASS SPECTROMETRY: [Corazonin]: Mass=1369.63; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:15174081};
CC -!- SIMILARITY: Belongs to the corazonin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB32283.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF55046.1; -; Genomic_DNA.
DR EMBL; S74038; AAB32283.1; ALT_SEQ; Genomic_DNA.
DR PIR; JC2384; JC2384.
DR RefSeq; NP_524350.1; NM_079626.3.
DR AlphaFoldDB; Q26377; -.
DR BioGRID; 66818; 3.
DR STRING; 7227.FBpp0082386; -.
DR PaxDb; Q26377; -.
DR PRIDE; Q26377; -.
DR EnsemblMetazoa; FBtr0082927; FBpp0082386; FBgn0013767.
DR GeneID; 41742; -.
DR KEGG; dme:Dmel_CG3302; -.
DR UCSC; CG3302-RA; d. melanogaster.
DR CTD; 12973; -.
DR FlyBase; FBgn0013767; Crz.
DR VEuPathDB; VectorBase:FBgn0013767; -.
DR eggNOG; ENOG502T821; Eukaryota.
DR HOGENOM; CLU_1679760_0_0_1; -.
DR InParanoid; Q26377; -.
DR OMA; RHRQSNE; -.
DR OrthoDB; 1457906at2759; -.
DR PhylomeDB; Q26377; -.
DR BioGRID-ORCS; 41742; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41742; -.
DR PRO; PR:Q26377; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0013767; Expressed in brain and 10 other tissues.
DR Genevisible; Q26377; DM.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0071858; F:corazonin receptor binding; IPI:FlyBase.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISS:UniProtKB.
DR GO; GO:0006117; P:acetaldehyde metabolic process; IMP:FlyBase.
DR GO; GO:0048149; P:behavioral response to ethanol; IMP:FlyBase.
DR GO; GO:0071361; P:cellular response to ethanol; IMP:FlyBase.
DR GO; GO:0007619; P:courtship behavior; IMP:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IDA:FlyBase.
DR GO; GO:0045823; P:positive regulation of heart contraction; ISS:UniProtKB.
DR InterPro; IPR020190; Procorazonin.
DR Pfam; PF17308; Corazonin; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Pyrrolidone carboxylic acid; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:12171930"
FT CHAIN 20..154
FT /note="Pro-corazonin"
FT /evidence="ECO:0000255"
FT /id="PRO_0000341610"
FT PEPTIDE 20..30
FT /note="Corazonin"
FT /id="PRO_0000000948"
FT PEPTIDE 34..67
FT /note="Corazonin precursor-related peptide"
FT /id="PRO_0000000949"
FT PROPEP 70..154
FT /id="PRO_0000000950"
FT REGION 91..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..119
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 20
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:12171930"
FT MOD_RES 30
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:12171930"
FT CONFLICT 81
FT /note="L -> Q (in Ref. 3; AAB32283)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 154 AA; 17154 MW; 06046282A1C61A2F CRC64;
MLRLLLLPLF LFTLSMCMGQ TFQYSRGWTN GKRSFNAASP LLANGHLHRA SELGLTDLYD
LQDWSSDRRL ERCLSQLQRS LIARNCVPGS DFNANRVDPD PENSAHPRLS NSNGENVLYS
SANIPNRHRQ SNELLEELSA AGGASAEPNV FGKH
