ID   COS3_YEAST              Reviewed;         379 AA.
AC   P0CX13; D6VQU7; P38363;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Protein COS3;
GN   Name=COS3; OrderedLocusNames=YML132W; ORFNames=YM4987.03;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the DUP/COS family. {ECO:0000305}.
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DR   EMBL; Z50178; CAA90551.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA09767.1; -; Genomic_DNA.
DR   PIR; S46187; S46187.
DR   RefSeq; NP_009861.1; NM_001178650.1.
DR   RefSeq; NP_013574.1; NM_001182495.1.
DR   AlphaFoldDB; P0CX13; -.
DR   BioGRID; 32994; 1.
DR   BioGRID; 35073; 12.
DR   iPTMnet; P0CX13; -.
DR   EnsemblFungi; YBR302C_mRNA; YBR302C; YBR302C.
DR   EnsemblFungi; YML132W_mRNA; YML132W; YML132W.
DR   GeneID; 852605; -.
DR   GeneID; 854907; -.
DR   KEGG; sce:YBR302C; -.
DR   KEGG; sce:YML132W; -.
DR   SGD; S000004601; COS3.
DR   VEuPathDB; FungiDB:YBR302C; -.
DR   VEuPathDB; FungiDB:YML132W; -.
DR   GeneTree; ENSGT00940000176283; -.
DR   HOGENOM; CLU_062892_1_0_1; -.
DR   InParanoid; P0CX13; -.
DR   BioCyc; YEAST:G3O-32710-MON; -.
DR   PRO; PR:P0CX13; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; P0CX13; protein.
DR   ExpressionAtlas; P0CX13; baseline.
DR   GO; GO:0005768; C:endosome; IDA:SGD.
DR   GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0006883; P:cellular sodium ion homeostasis; IMP:SGD.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISS:SGD.
DR   InterPro; IPR001142; DUP/COS.
DR   Pfam; PF00674; DUP; 2.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..379
FT                   /note="Protein COS3"
FT                   /id="PRO_0000409750"
FT   TOPO_DOM        1..72
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        94..254
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        276..379
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   379 AA;  45165 MW;  2AAB51B6C103AF61 CRC64;
     MKENELKNEK SVDVLSFKQL ESQKIVLPQD LFRSSFTWFC YEIYKSLAFR IWMLLWLPLS
     VWWKLSNNCI YPLIVSLLVL FLGPIFVLVI CGLSRKRSLS KQLIQFCKEI TENTPSSDPH
     DWEVVAANLN SYLYENNVWN TKYFFFNAMV CQEAFRTTLL EPFSLKKDKA AKVKSFKDSV
     PYIEEALGVY FTEVEKQWKL FNTEKSWSPV GLEDAKLPKE AYRFKLTWFL KRISNIFMLI
     PFLNFLCCIY VSRGMCLLLR TLYLGWILFM LVQGFQNIRV LIMSMEHKMQ FLSTIINEQE
     SGANGWDEIA RKMNRYLFEK KAWKNEEFFF DGIDCEWFFN HFFYRVLSAK KSMWPLPLNV
     ELWPYIKEAQ LSRSEVLLV