COS5_YEAST
ID COS5_YEAST Reviewed; 383 AA.
AC P47187; D6VWX9;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein COS5;
GN Name=COS5; OrderedLocusNames=YJR161C; ORFNames=J2410;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- MISCELLANEOUS: Present with 815 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DUP/COS family. {ECO:0000305}.
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DR EMBL; Z49661; CAA89694.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08945.1; -; Genomic_DNA.
DR PIR; S57191; S57191.
DR RefSeq; NP_012695.3; NM_001181819.3.
DR AlphaFoldDB; P47187; -.
DR BioGRID; 33915; 70.
DR DIP; DIP-7521N; -.
DR STRING; 4932.YJR161C; -.
DR iPTMnet; P47187; -.
DR MaxQB; P47187; -.
DR PaxDb; P47187; -.
DR EnsemblFungi; YJR161C_mRNA; YJR161C; YJR161C.
DR GeneID; 853626; -.
DR KEGG; sce:YJR161C; -.
DR SGD; S000003922; COS5.
DR VEuPathDB; FungiDB:YJR161C; -.
DR eggNOG; ENOG502SAGH; Eukaryota.
DR GeneTree; ENSGT00940000176283; -.
DR HOGENOM; CLU_062892_1_0_1; -.
DR InParanoid; P47187; -.
DR OMA; FLATIMH; -.
DR BioCyc; YEAST:G3O-31770-MON; -.
DR PRO; PR:P47187; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47187; protein.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:SGD.
DR InterPro; IPR001142; DUP/COS.
DR Pfam; PF00674; DUP; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..383
FT /note="Protein COS5"
FT /id="PRO_0000207516"
FT TOPO_DOM 1..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..72
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..383
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 383 AA; 45709 MW; C8E3D0A4AEA394B2 CRC64;
MKENELKNEK SVDVLSFKQL ESQKIVLPQD LFRSSFTWFC YEIYKSLAFP IWMLLWLPLS
VWWKLSNNCI YPLIVSLLVL FLGPIFVLVI CGLSRKRSLS KQLIQFCKEV TENTPSSDPH
DWEVVAANLN SYLYENKAWN TRYFFFNAMG CQEAFRTTLL EPFSLKKDEA AKVKSFKDSV
PYIEEALGVY FREVEKQWKL FNTEKSWSPV GLEDVQLPKD IHRSKLTWFL KRIFTIYSLP
LWLAFLNCIC VSQHFCLAFR ILCPGLFFLM MVWLFQNMRT TALLVKMEHK MQFLLTIINE
QESGANGWDE IARKMNRYLF EKKAWKNEEF FFEGIDCEWF FSHFFYRLLS AKKSMWLLPL
NVELWPYIKE AQLSRNEESL MKK
