COS8_YEAST
ID COS8_YEAST Reviewed; 381 AA.
AC P38723; D3DKS3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein COS8;
GN Name=COS8; OrderedLocusNames=YHL048W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- MISCELLANEOUS: Present with 3070 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DUP/COS family. {ECO:0000305}.
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DR EMBL; U11583; AAB65060.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06640.1; -; Genomic_DNA.
DR PIR; S48920; S48920.
DR RefSeq; NP_011815.1; NM_001179128.1.
DR AlphaFoldDB; P38723; -.
DR BioGRID; 36377; 66.
DR DIP; DIP-4866N; -.
DR IntAct; P38723; 8.
DR MINT; P38723; -.
DR STRING; 4932.YHL048W; -.
DR iPTMnet; P38723; -.
DR MaxQB; P38723; -.
DR PaxDb; P38723; -.
DR PRIDE; P38723; -.
DR EnsemblFungi; YHL048W_mRNA; YHL048W; YHL048W.
DR GeneID; 856337; -.
DR KEGG; sce:YHL048W; -.
DR SGD; S000001040; COS8.
DR VEuPathDB; FungiDB:YHL048W; -.
DR eggNOG; ENOG502SAGH; Eukaryota.
DR GeneTree; ENSGT00940000176283; -.
DR HOGENOM; CLU_062892_1_0_1; -.
DR InParanoid; P38723; -.
DR OMA; FFFNAMS; -.
DR BioCyc; YEAST:G3O-31064-MON; -.
DR PRO; PR:P38723; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38723; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:SGD.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISS:SGD.
DR InterPro; IPR001142; DUP/COS.
DR Pfam; PF00674; DUP; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..381
FT /note="Protein COS8"
FT /id="PRO_0000207519"
FT TOPO_DOM 1..42
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..237
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 259..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 381 AA; 45880 MW; 6BCEDC526743F504 CRC64;
MKENEVKDEK SVDVLSFKQL EFQKTVLPQD VFRNELTWFC YEIYKSLAFR IWMLLWLPLS
VWWKLSSNWI HPLIVSLLVL FLGPFFVLVI CGLSRKRSLS KQLIQFCKEI TEDTPSSDPH
DWEVVAANLN SYFYENKTWN TKYFFFNAMS CQKAFKTTLL EPFSLKKDES AKVKSFKDSV
PYIEEALQVY AAGFDKEWKL FNTEKEESPF DLEDIQLPKE AYRFKLTWIL KRIFNLRCLP
LFLYYFLIVY TSGNADLISR FLFPVVMFFI MTRDFQNMRM IVLSVKMEHK MQFLSTIINE
QESGANGWDE IAKKMNRYLF EKKVWNNEEF FYDGLDCEWF FRRFFYRLLS LKKPMWFASL
NVELWPYIKE AQSARNEKPL K
