ID   COTE_BACSU              Reviewed;         181 AA.
AC   P14016;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Spore coat protein E;
GN   Name=cotE; OrderedLocusNames=BSU17030;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN PROTEIN ASSEMBLY OF THE
RP   SPORE COAT.
RX   PubMed=3139490; DOI=10.1101/gad.2.8.1047;
RA   Zheng L., Donovan W.P., Fitz-James P.C., Losick R.;
RT   "Gene encoding a morphogenic protein required in the assembly of the outer
RT   coat of the Bacillus subtilis endospore.";
RL   Genes Dev. 2:1047-1054(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
RX   PubMed=1691789; DOI=10.1016/0022-2836(90)90227-d;
RA   Zheng L., Losick R.;
RT   "Cascade regulation of spore coat gene expression in Bacillus subtilis.";
RL   J. Mol. Biol. 212:645-660(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 164-181.
RC   STRAIN=168;
RX   PubMed=8760914; DOI=10.1099/13500872-142-8-2021;
RA   Ginetti F., Perego M., Albertini A.M., Galizzi A.;
RT   "Bacillus subtilis mutS mutL operon: identification, nucleotide sequence
RT   and mutagenesis.";
RL   Microbiology 142:2021-2029(1996).
RN   [5]
RP   SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RC   STRAIN=168 / PY79;
RX   PubMed=8299942; DOI=10.1101/gad.8.2.234;
RA   Driks A., Roels S., Beall B., Moran C.P. Jr., Losick R.;
RT   "Subcellular localization of proteins involved in the assembly of the spore
RT   coat of Bacillus subtilis.";
RL   Genes Dev. 8:234-244(1994).
RN   [6]
RP   FUNCTIONAL REGIONS.
RC   STRAIN=168 / PY79;
RX   PubMed=10559171; DOI=10.1128/jb.181.22.7043-7051.1999;
RA   Bauer T., Little S., Stoever A.G., Driks A.;
RT   "Functional regions of the Bacillus subtilis spore coat morphogenetic
RT   protein CotE.";
RL   J. Bacteriol. 181:7043-7051(1999).
RN   [7]
RP   FUNCTIONAL REGIONS OF THE C-TERMINUS, AND SUBUNIT.
RC   STRAIN=168 / PY79;
RX   PubMed=11737650; DOI=10.1046/j.1365-2958.2001.02708.x;
RA   Little S., Driks A.;
RT   "Functional analysis of the Bacillus subtilis morphogenetic spore coat
RT   protein CotE.";
RL   Mol. Microbiol. 42:1107-1120(2001).
CC   -!- FUNCTION: Morphogenic protein required for the assembly of the outer
CC       coat of the endospore. Is also a regulatory protein for the expression
CC       of cotA, cotB, cotC, cotH and other genes encoding spore outer coat
CC       proteins. {ECO:0000269|PubMed:10559171, ECO:0000269|PubMed:11737650,
CC       ECO:0000269|PubMed:3139490}.
CC   -!- SUBUNIT: Homooligomer. {ECO:0000269|PubMed:11737650}.
CC   -!- INTERACTION:
CC       P14016; P14016: cotE; NbExp=2; IntAct=EBI-6407152, EBI-6407152;
CC       P14016; P37963: spoVID; NbExp=2; IntAct=EBI-6407152, EBI-6419142;
CC   -!- SUBCELLULAR LOCATION: Spore coat {ECO:0000269|PubMed:8299942}.
CC       Note=Localizes between the two coat layers in the mature spore.
CC   -!- DEVELOPMENTAL STAGE: Expressed during sporulation.
CC       {ECO:0000269|PubMed:8299942}.
CC   -!- SIMILARITY: Belongs to the CotE family. {ECO:0000305}.
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DR   EMBL; X13009; CAA31433.1; ALT_TERM; Genomic_DNA.
DR   EMBL; AL009126; CAB13576.1; -; Genomic_DNA.
DR   EMBL; U27343; AAB19234.1; -; Genomic_DNA.
DR   PIR; A31245; A31245.
DR   RefSeq; NP_389585.1; NC_000964.3.
DR   RefSeq; WP_003231833.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P14016; -.
DR   IntAct; P14016; 3.
DR   STRING; 224308.BSU17030; -.
DR   PaxDb; P14016; -.
DR   PRIDE; P14016; -.
DR   EnsemblBacteria; CAB13576; CAB13576; BSU_17030.
DR   GeneID; 939508; -.
DR   KEGG; bsu:BSU17030; -.
DR   PATRIC; fig|224308.179.peg.1844; -.
DR   eggNOG; ENOG502ZIKA; Bacteria.
DR   OMA; GCWIINH; -.
DR   BioCyc; BSUB:BSU17030-MON; -.
DR   BRENDA; 1.1.1.62; 2681.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR018901; Spore_coat_CotE.
DR   Pfam; PF10628; CotE; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Sporulation.
FT   CHAIN           1..181
FT                   /note="Spore coat protein E"
FT                   /id="PRO_0000079262"
FT   REGION          1..101
FT                   /note="Required for deposition of coat proteins"
FT   REGION          58..75
FT                   /note="Required for oligomerization"
FT   REGION          102..144
FT                   /note="Required for targeting to the spore"
FT   REGION          156..158
FT                   /note="Required for the assembly of CotA, CotR and YaaH"
FT   REGION          159..169
FT                   /note="Required for the assembly of CotG and CotB"
FT   REGION          176..178
FT                   /note="Required for the assembly of CotS and CotSA"
SQ   SEQUENCE   181 AA;  20977 MW;  6E9FBAE3E059BFC2 CRC64;
     MSEYREIITK AVVAKGRKFT QCTNTISPEK KPSSILGGWI INHKYDAEKI GKTVEIEGYY
     DINVWYSYAD NTKTEVVTER VKYVDVIKLR YRDNNYLDDE HEVIAKVLQQ PNCLEVTISP
     NGNKIVVQAE REFLAEVVGE TKVVVEVNPD WEEDDEEDWE DELDEELEDI NPEFLVGDPE
     E