COTF_BACSU
ID COTF_BACSU Reviewed; 160 AA.
AC P23261;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Spore coat protein F;
DE Contains:
DE RecName: Full=5 kDa coat protein;
DE Contains:
DE RecName: Full=8 kDa coat protein;
DE Flags: Precursor;
GN Name=cotF; OrderedLocusNames=BSU40530;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 5-20 AND 77-81, AND
RP SUBCELLULAR LOCATION.
RX PubMed=1708381; DOI=10.1128/jb.173.9.2915-2919.1991;
RA Cutting S.M., Zheng L., Losick R.;
RT "Gene encoding two alkali-soluble components of the spore coat from
RT Bacillus subtilis.";
RL J. Bacteriol. 173:2915-2919(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP PROTEIN SEQUENCE OF 1-8, AND AMOUNT IN YABG MUTANT SPORES.
RC STRAIN=168;
RX PubMed=10714992; DOI=10.1128/jb.182.7.1883-1888.2000;
RA Takamatsu H., Kodama T., Imamura A., Asai K., Kobayashi K., Nakayama T.,
RA Ogasawara N., Watabe K.;
RT "The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase
RT during sporulation, and yabG mutant spores have altered coat protein
RT composition.";
RL J. Bacteriol. 182:1883-1888(2000).
CC -!- SUBCELLULAR LOCATION: Spore coat {ECO:0000269|PubMed:1708381}.
CC -!- PTM: Generates the two 5 and 8 kDa components of the spore coat, after
CC proteolytic processing.
CC -!- MISCELLANEOUS: Present in an increased level in yabG mutant spores.
CC -!- SIMILARITY: Belongs to the CotF family. {ECO:0000305}.
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DR EMBL; M58592; AAA22813.1; -; Genomic_DNA.
DR EMBL; D26185; BAA05184.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB16090.1; -; Genomic_DNA.
DR PIR; S65978; S65978.
DR RefSeq; NP_391933.1; NC_000964.3.
DR RefSeq; WP_003243364.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P23261; -.
DR SMR; P23261; -.
DR STRING; 224308.BSU40530; -.
DR PaxDb; P23261; -.
DR PRIDE; P23261; -.
DR EnsemblBacteria; CAB16090; CAB16090; BSU_40530.
DR GeneID; 936671; -.
DR KEGG; bsu:BSU40530; -.
DR PATRIC; fig|224308.179.peg.4389; -.
DR eggNOG; COG5577; Bacteria.
DR OMA; DVRNANR; -.
DR BioCyc; BSUB:BSU40530-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.10; -; 1.
DR InterPro; IPR012347; Ferritin-like.
DR InterPro; IPR016493; Spore_coat_CotF.
DR InterPro; IPR012851; Spore_coat_CotF-like.
DR PANTHER; PTHR39183; PTHR39183; 1.
DR Pfam; PF07875; Coat_F; 1.
DR PIRSF; PIRSF006716; Spore_coat_CotF; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Sporulation.
FT PROPEP 1..4
FT /evidence="ECO:0000255"
FT /id="PRO_0000020983"
FT CHAIN 5..76
FT /note="5 kDa coat protein"
FT /id="PRO_0000020984"
FT CHAIN 77..160
FT /note="8 kDa coat protein"
FT /id="PRO_0000020985"
SQ SEQUENCE 160 AA; 18725 MW; F3F7869A26D56916 CRC64;
MDERRTLAWH ETLEMHELVA FQSNGLIKLK KMIREVKDPQ LRQLYNVSIQ GVEQNLRELL
PFFPQAPHRE DEEEERADNP FYSGDLLGFA KTSVRSYAIA ITETATPQLR NVLVKQLNAA
IQLHAQVYRY MYQHGYYPSY NLSELLKNDV RNANRAISMK
