COTH_BACSU
ID COTH_BACSU Reviewed; 362 AA.
AC Q45535; O05221;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Inner spore coat protein H;
GN Name=cotH; Synonyms=ywrH; OrderedLocusNames=BSU36060;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=168 / PY17;
RX PubMed=8755863; DOI=10.1128/jb.178.15.4375-4380.1996;
RA Naclerio G., Baccigalupi L., Zilhao R., de Felice M., Ricca E.;
RT "Bacillus subtilis spore coat assembly requires cotH gene expression.";
RL J. Bacteriol. 178:4375-4380(1996).
RN [2]
RP ERRATUM OF PUBMED:8755863.
RX PubMed=8991897; DOI=10.1128/jb.178.21.6407-6407.1996;
RA Naclerio G., Baccigalupi L., Zilhao R., de Felice M., Ricca E.;
RL J. Bacteriol. 178:6407-6407(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.
RC STRAIN=168 / PY79;
RX PubMed=7814326; DOI=10.1128/jb.177.2.372-377.1995;
RA Sacco M., Ricca E., Losick R., Cutting S.M.;
RT "An additional GerE-controlled gene encoding an abundant spore coat protein
RT from Bacillus subtilis.";
RL J. Bacteriol. 177:372-377(1995).
RN [6]
RP CHARACTERIZATION.
RX PubMed=10198031; DOI=10.1128/jb.181.8.2631-2633.1999;
RA Zilhao R., Naclerio G., Henriques A.O., Baccigalupi L., Moran C.P. Jr.,
RA Ricca E.;
RT "Assembly requirements and role of CotH during spore coat formation in
RT Bacillus subtilis.";
RL J. Bacteriol. 181:2631-2633(1999).
RN [7]
RP FUNCTION.
RX PubMed=14762006; DOI=10.1128/jb.186.4.1110-1119.2004;
RA Zilhao R., Serrano M., Isticato R., Ricca E., Moran C.P. Jr.,
RA Henriques A.O.;
RT "Interactions among CotB, CotG, and CotH during assembly of the Bacillus
RT subtilis spore coat.";
RL J. Bacteriol. 186:1110-1119(2004).
RN [8]
RP FUNCTION.
RX PubMed=14762008; DOI=10.1128/jb.186.4.1129-1135.2004;
RA Isticato R., Esposito G., Zilhao R., Nolasco S., Cangiano G., De Felice M.,
RA Henriques A.O., Ricca E.;
RT "Assembly of multiple CotC forms into the Bacillus subtilis spore coat.";
RL J. Bacteriol. 186:1129-1135(2004).
RN [9]
RP INDUCTION, AND FUNCTION.
RX PubMed=15470121; DOI=10.1099/mic.0.27356-0;
RA Baccigalupi L., Castaldo G., Cangiano G., Isticato R., Marasco R.,
RA De Felice M., Ricca E.;
RT "GerE-independent expression of cotH leads to CotC accumulation in the
RT mother cell compartment during Bacillus subtilis sporulation.";
RL Microbiology 150:3441-3449(2004).
RN [10]
RP FUNCTION.
RX PubMed=18065538; DOI=10.1128/jb.01425-07;
RA Isticato R., Pelosi A., Zilhao R., Baccigalupi L., Henriques A.O.,
RA De Felice M., Ricca E.;
RT "CotC-CotU heterodimerization during assembly of the Bacillus subtilis
RT spore coat.";
RL J. Bacteriol. 190:1267-1275(2008).
CC -!- FUNCTION: Involved in the assembly of several proteins in the inner and
CC outer layer of the spore coat. Stabilizes CotC and CotU in the mother
CC cell compartment of sporulating cells and promotes the assembly of both
CC early and late forms of CotC-related polypeptides on the spore surface.
CC {ECO:0000269|PubMed:14762006, ECO:0000269|PubMed:14762008,
CC ECO:0000269|PubMed:15470121, ECO:0000269|PubMed:18065538,
CC ECO:0000269|PubMed:8755863}.
CC -!- SUBUNIT: Assembly of CotH requires CotE and GerE.
CC -!- SUBCELLULAR LOCATION: Spore coat {ECO:0000269|PubMed:8755863}.
CC -!- INDUCTION: Expression is negatively regulated by the transcriptional
CC regulator GerE. {ECO:0000269|PubMed:15470121}.
CC -!- SIMILARITY: Belongs to the CotH family. {ECO:0000305}.
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DR EMBL; X98342; CAA66988.1; -; Genomic_DNA.
DR EMBL; Z93767; CAB07793.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15623.1; -; Genomic_DNA.
DR EMBL; U14964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; E69605; E69605.
DR RefSeq; NP_391487.1; NC_000964.3.
DR RefSeq; WP_003227854.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; Q45535; -.
DR SMR; Q45535; -.
DR STRING; 224308.BSU36060; -.
DR PaxDb; Q45535; -.
DR PRIDE; Q45535; -.
DR EnsemblBacteria; CAB15623; CAB15623; BSU_36060.
DR GeneID; 936858; -.
DR KEGG; bsu:BSU36060; -.
DR PATRIC; fig|224308.179.peg.3903; -.
DR eggNOG; COG5337; Bacteria.
DR InParanoid; Q45535; -.
DR OMA; HLNAEYK; -.
DR PhylomeDB; Q45535; -.
DR BioCyc; BSUB:BSU36060-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR014867; Spore_coat_CotH_CotH2/3/7.
DR PANTHER; PTHR40050; PTHR40050; 2.
DR Pfam; PF08757; CotH; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Sporulation.
FT CHAIN 1..362
FT /note="Inner spore coat protein H"
FT /id="PRO_0000079264"
FT CONFLICT 75
FT /note="A -> G (in Ref. 1; CAA66988)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 362 AA; 42813 MW; 79C5E30BA01B3311 CRC64;
MKNQSNLPLY QLFVHPKDLR ELKKDIWDDD PVPAVMKVNQ KRLDIDIAYR GSHIRDFKKK
SYHISFYQPK TFRGAREIHL NAEYKDPSLM RNKLSLDFFS ELGTLSPKAE FAFVKMNGKN
EGVYLELESV DEYYLAKRKL ADGAIFYAVD DDANFSLMSD LERETKTSLE LGYEKKTGTE
EDDFYLQDMI FKINTVPKAQ FKSEVTKHVD VDKYLRWLAG IVFTSNYDGF VHNYALYRSG
ETGLFEVIPW DYDATWGRDI HGERMAADYV RIQGFNTLTA RILDESEFRK SYKRLLEKTL
QSLFTIEYME PKIMAMYERI RPFVLMDPYK KNDIERFDRE PDVICEYIKN RSQYLKDHLS
IL
