ID   COTR_BACSU              Reviewed;         320 AA.
AC   O06996; Q795H2;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Putative sporulation hydrolase CotR;
DE            EC=3.1.1.-;
GN   Name=cotR; Synonyms=yvdO; OrderedLocusNames=BSU34530;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Denizot F.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC   STRAIN=168 / PY79;
RX   PubMed=11737650; DOI=10.1046/j.1365-2958.2001.02708.x;
RA   Little S., Driks A.;
RT   "Functional analysis of the Bacillus subtilis morphogenetic spore coat
RT   protein CotE.";
RL   Mol. Microbiol. 42:1107-1120(2001).
CC   -!- SUBCELLULAR LOCATION: Spore coat {ECO:0000305|PubMed:11737650}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during sporulation, in the late phase of
CC       coat protein synthesis. {ECO:0000269|PubMed:11737650}.
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DR   EMBL; Z94043; CAB08044.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15458.1; -; Genomic_DNA.
DR   PIR; F70034; F70034.
DR   RefSeq; NP_391333.1; NC_000964.3.
DR   RefSeq; WP_003243674.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O06996; -.
DR   SMR; O06996; -.
DR   STRING; 224308.BSU34530; -.
DR   PaxDb; O06996; -.
DR   PRIDE; O06996; -.
DR   EnsemblBacteria; CAB15458; CAB15458; BSU_34530.
DR   GeneID; 936427; -.
DR   KEGG; bsu:BSU34530; -.
DR   PATRIC; fig|224308.179.peg.3740; -.
DR   eggNOG; COG3621; Bacteria.
DR   InParanoid; O06996; -.
DR   OMA; HNFPGSP; -.
DR   PhylomeDB; O06996; -.
DR   BioCyc; BSUB:BSU34530-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR002641; PNPLA_dom.
DR   Pfam; PF01734; Patatin; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS51635; PNPLA; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Reference proteome;
KW   Sporulation.
FT   CHAIN           1..320
FT                   /note="Putative sporulation hydrolase CotR"
FT                   /id="PRO_0000360659"
FT   DOMAIN          7..182
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           11..16
FT                   /note="GXGXXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           42..46
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        44
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        169
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
SQ   SEQUENCE   320 AA;  35357 MW;  15909FEA37BC3125 CRC64;
     MAKYRIMTFD GGGTLGALSL QLLNRLARQN PKLISRTHVF SGNSIGSFTA LALASGRSPR
     ETLQYFEDEI LPAFSISRPG GPVFNQQLPY SGFIKAVRNF FPADLQLIDL RKRIVVPSFK
     LYSQKLDRWT PVLFHNFPGS PYLNEKVSDV ILRSSGAPAT QRAYQNYVDG YVVATNPSTA
     SIAFAVGKAN VPLDQIAVLS IGTGEAPTRL RRDTRGWGMV SADNIRPENL KNLPPNWGVL
     LDRSPNEPLL PFLQMIAGGN GYYESMVSAN LLGDRFFRLD PRIPNFSKTD PAVVPAVIEI
     ANKTNLQPAN QFIEKNWGSK