COTS_BACSU
ID COTS_BACSU Reviewed; 351 AA.
AC P46914;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Spore coat protein S;
DE AltName: Full=Coat protein 40 kDa component 2;
DE Short=COT40-2;
GN Name=cotS; OrderedLocusNames=BSU30900;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / 60015;
RX PubMed=7545510; DOI=10.1099/13500872-141-6-1433;
RA Abe A., Koide H., Kohno T., Watabe K.;
RT "A Bacillus subtilis spore coat polypeptide gene, cotS.";
RL Microbiology 141:1433-1442(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP PROTEIN SEQUENCE OF 1-30.
RX PubMed=8289687; DOI=10.1111/j.1348-0421.1993.tb01709.x;
RA Abe A., Ogawa S., Kohno T., Watabe K.;
RT "Purification of Bacillus subtilis spore coat protein by electrophoretic
RT elution procedure and determination of NH2-terminal amino acid sequences.";
RL Microbiol. Immunol. 37:809-812(1993).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12562816; DOI=10.1128/jb.185.4.1443-1454.2003;
RA Lai E.-M., Phadke N.D., Kachman M.T., Giorno R., Vazquez S., Vazquez J.A.,
RA Maddock J.R., Driks A.;
RT "Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus
RT anthracis.";
RL J. Bacteriol. 185:1443-1454(2003).
CC -!- FUNCTION: Seems to be required for the assembly of the CotSA protein in
CC spores.
CC -!- SUBCELLULAR LOCATION: Spore coat {ECO:0000269|PubMed:12562816}.
CC -!- DEVELOPMENTAL STAGE: Late stage of sporulation.
CC -!- SIMILARITY: Belongs to the CotS family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D31847; BAA06634.1; -; Genomic_DNA.
DR EMBL; AF008220; AAC00220.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15068.1; -; Genomic_DNA.
DR PIR; I39808; I39808.
DR RefSeq; NP_390968.1; NC_000964.3.
DR RefSeq; WP_003229031.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P46914; -.
DR SMR; P46914; -.
DR STRING; 224308.BSU30900; -.
DR PaxDb; P46914; -.
DR EnsemblBacteria; CAB15068; CAB15068; BSU_30900.
DR GeneID; 936628; -.
DR KEGG; bsu:BSU30900; -.
DR PATRIC; fig|224308.179.peg.3349; -.
DR eggNOG; COG2334; Bacteria.
DR OMA; KWHKLYR; -.
DR BioCyc; BSUB:BSU30900-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0042601; C:endospore-forming forespore; IBA:GO_Central.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR014255; Spore_coat_CotS.
DR SUPFAM; SSF56112; SSF56112; 1.
DR TIGRFAMs; TIGR02906; spore_CotS; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Sporulation.
FT CHAIN 1..351
FT /note="Spore coat protein S"
FT /id="PRO_0000079269"
FT CONFLICT 9
FT /note="Q -> E (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 351 AA; 41084 MW; 7F6DEF041417B26D CRC64;
MYQKEHEEQI VSEILSYYPF HIDHVALKSN KSGRKIWEVE TDHGPKLLKE AQMKPERMLF
ITQAHAHLQE KGLPIAPIHQ TKNGGSCLGT DQVSYSLYDK VTGKEMIYYD AEQMKKVMSF
AGHFHHASKG YVCTDESKKR SRLGKWHKLY RWKLQELEGN MQIAASYPDD VFSQTFLKHA
DKMLARGKEA LRALDDSEYE TWTKETLEHG GFCFQDFTLA RLTEIEGEPF LKELHSITYD
LPSRDLRILL NKVMVKLSVW DTDFMVALLA AYDAVYPLTE KQYEVLWIDL AFPHLFCAIG
HKYYLKQKKT WSDEKYNWAL QNMISVEESK DSFLDKLPEL YKKIKAYREA N
