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2B_CMVFN
ID   2B_CMVFN                Reviewed;         110 AA.
AC   P0C783;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   02-JUN-2021, entry version 21.
DE   RecName: Full=Suppressor of silencing 2b;
DE   AltName: Full=Protein 2b;
GN   ORFNames=ORF2b;
OS   Cucumber mosaic virus (strain FNY) (CMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=12307;
OH   NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3404113; DOI=10.1099/0022-1317-69-8-1777;
RA   Rizzo T.M., Palukaitis P.;
RT   "Nucleotide sequence and evolutionary relationships of cucumber mosaic
RT   virus (CMV) strains: CMV RNA 2.";
RL   J. Gen. Virol. 69:1777-1787(1988).
RN   [2]
RP   FUNCTION, AND INTERACTION WITH ARABIDOPSIS ARGONAUTE1/AGO1.
RX   PubMed=17158744; DOI=10.1101/gad.1495506;
RA   Zhang X., Yuan Y.R., Pei Y., Lin S.S., Tuschl T., Patel D.J., Chua N.H.;
RT   "Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis
RT   Argonaute1 cleavage activity to counter plant defense.";
RL   Genes Dev. 20:3255-3268(2006).
RN   [3]
RP   FUNCTION, AND MUTAGENESIS OF 1-MET--LEU-3; 22-LYS--GLN-24; 10-ASN--GLU-12;
RP   31-GLN--ARG-33; 34-ARG--ARG-36; 40-SER--SER-42; 55-LEU--PHE-57 AND
RP   70-ARG--VAL-72.
RX   PubMed=25380036; DOI=10.1371/journal.pone.0112095;
RA   Nemes K., Gellert A., Balazs E., Salanki K.;
RT   "Alanine scanning of cucumber mosaic virus (CMV) 2b protein identifies
RT   different positions for cell-to-cell movement and gene silencing suppressor
RT   activity.";
RL   PLoS ONE 9:E112095-E112095(2014).
CC   -!- FUNCTION: Acts as suppressor of RNA-mediated gene silencing, also known
CC       as post-transcriptional gene silencing (PTGS), a mechanism of plant
CC       viral defense that limits the accumulation of viral RNAs. Suppresses
CC       RNA silencing by direct perturbation of host AGO1 slicer activity and
CC       thus interfering with miRNA pathways. {ECO:0000250|UniProtKB:Q66125,
CC       ECO:0000269|PubMed:25380036}.
CC   -!- SUBUNIT: Homotetramer (Probable). Interacts with host AGO1; this
CC       interaction blocks AGO1 cleavage activity to attenuate RNA silencing
CC       and thus counter host defense (PubMed:17158744).
CC       {ECO:0000269|PubMed:17158744, ECO:0000305|PubMed:25380036}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:Q66125}.
CC   -!- SIMILARITY: Belongs to the cucumovirus/ilarvirus protein 2b family.
CC       {ECO:0000305}.
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DR   EMBL; D00355; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   SMR; P0C783; -.
DR   IntAct; P0C783; 1.
DR   Proteomes; UP000002502; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004946; Cucumo_2B.
DR   Pfam; PF03263; Cucumo_2B; 1.
PE   1: Evidence at protein level;
KW   Host nucleus; Reference proteome; Suppressor of RNA silencing.
FT   CHAIN           1..110
FT                   /note="Suppressor of silencing 2b"
FT                   /id="PRO_0000399910"
FT   REGION          16..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           22..27
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q66125"
FT   MUTAGEN         1..3
FT                   /note="MEL->AAA: Loss of cell-to-cell movement."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         10..12
FT                   /note="NVE->AAA: Loss of gene silencing suppressor
FT                   function."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         22..24
FT                   /note="KKQ->AAA: Loss of gene silencing suppressor
FT                   function."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         31..33
FT                   /note="QNR->AAA: Loss of gene silencing suppressor
FT                   function."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         34..36
FT                   /note="RER->AAA: Loss of gene silencing suppressor
FT                   function."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         40..42
FT                   /note="SPS->AAA: Loss of gene silencing suppressor
FT                   function."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         55..57
FT                   /note="LPF->AAA: Loss of gene silencing suppressor
FT                   function."
FT                   /evidence="ECO:0000269|PubMed:25380036"
FT   MUTAGEN         70..72
FT                   /note="RHV->AAA: Loss of cell-to-cell movement."
FT                   /evidence="ECO:0000269|PubMed:25380036"
SQ   SEQUENCE   110 AA;  12749 MW;  59690777E7D64128 CRC64;
     MELNVGAMTN VELQLARMVE AKKQRRRSHK QNRRERGHKS PSERARSNLR LFRFLPFYQV
     DGSELTGSCR HVNVAELPES EASRLELSAE DHDFDDTDWF AGNEWAEGAF
 
 
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