COW1_CONAO
ID COW1_CONAO Reviewed; 63 AA.
AC A0A1P8NVR5;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 25-MAY-2022, entry version 12.
DE RecName: Full=Contryphan-Ar2 {ECO:0000305};
DE AltName: Full=Ar1260 {ECO:0000303|PubMed:28152596};
DE AltName: Full=Ar1304 {ECO:0000303|PubMed:28152596};
DE Contains:
DE RecName: Full=Contryphan-Ar1 {ECO:0000305};
DE AltName: Full=Ar1131 {ECO:0000303|PubMed:28152596};
DE AltName: Full=Ar1175 {ECO:0000303|PubMed:28152596};
DE Flags: Precursor;
OS Conus araneosus (Cobweb cone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Conus.
OX NCBI_TaxID=101286;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 53-62 AND 54-62,
RP IDENTIFICATION BY MASS SPECTROMETRY, MASS SPECTROMETRY, SUBCELLULAR
RP LOCATION, DISULFIDE BOND, D-AMINO ACID AT TRP-58, AMIDATION AT CYS-62, AND
RP GAMMA-CARBOXYGLUTAMATION AT GLU-55.
RC TISSUE=Venom, and Venom duct;
RX PubMed=28152596; DOI=10.1021/acs.jproteome.6b00776;
RA Vijayasarathy M., Basheer S.M., Franklin J.B., Balaram P.;
RT "Contryphan genes and mature peptides in the venom of nine cone snail
RT species by transcriptomic and mass spectrometric analysis.";
RL J. Proteome Res. 16:763-772(2017).
CC -!- FUNCTION: Its target is unknown, but this toxin may modulate voltage-
CC activated calcium channels (Cav) or calcium-dependent potassium
CC channels (KCa). {ECO:0000250|UniProtKB:P0C248,
CC ECO:0000250|UniProtKB:P0C250, ECO:0000250|UniProtKB:P62903,
CC ECO:0000250|UniProtKB:P83047}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28152596}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC {ECO:0000305|PubMed:28152596}.
CC -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: [Contryphan-Ar2]: Mass=1260.62; Method=MALDI;
CC Note=Monoisotopic mass, Ar1260.;
CC Evidence={ECO:0000269|PubMed:28152596};
CC -!- MASS SPECTROMETRY: [Contryphan-Ar2]: Mass=1304.60; Method=MALDI;
CC Note=Monoisotopic mass, Ar1304 (gamma-carboxylated).;
CC Evidence={ECO:0000269|PubMed:28152596};
CC -!- MASS SPECTROMETRY: [Contryphan-Ar1]: Mass=1131.58; Method=MALDI;
CC Note=Monoisotopic mass, Ar1131.;
CC Evidence={ECO:0000269|PubMed:28152596};
CC -!- MASS SPECTROMETRY: [Contryphan-Ar1]: Mass=1175.56; Method=MALDI;
CC Note=Monoisotopic mass, Ar1175 (gamma-carboxylated).;
CC Evidence={ECO:0000269|PubMed:28152596};
CC -!- SIMILARITY: Belongs to the O2 superfamily. Contryphan family.
CC {ECO:0000305}.
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DR EMBL; KX289876; APX52853.1; -; mRNA.
DR AlphaFoldDB; A0A1P8NVR5; -.
DR ConoServer; 9752; Contryphan-Ar2 precursor.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR004214; Conotoxin.
DR InterPro; IPR011062; Contryphan_CS.
DR Pfam; PF02950; Conotoxin; 1.
DR PROSITE; PS60027; CONTRYPHAN; 1.
PE 1: Evidence at protein level;
KW Amidation; D-amino acid; Direct protein sequencing; Disulfide bond;
KW Gamma-carboxyglutamic acid; Ion channel impairing toxin; Neurotoxin;
KW Secreted; Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..52
FT /evidence="ECO:0000305|PubMed:28152596"
FT /id="PRO_0000445125"
FT PEPTIDE 53..62
FT /note="Contryphan-Ar2"
FT /evidence="ECO:0000269|PubMed:28152596"
FT /id="PRO_5012094458"
FT PEPTIDE 54..62
FT /note="Contryphan-Ar1"
FT /evidence="ECO:0000269|PubMed:28152596"
FT /id="PRO_0000445126"
FT MOD_RES 55
FT /note="4-carboxyglutamate; partial"
FT /evidence="ECO:0000305|PubMed:28152596"
FT MOD_RES 58
FT /note="D-tryptophan"
FT /evidence="ECO:0000305|PubMed:28152596"
FT MOD_RES 62
FT /note="Cysteine amide"
FT /evidence="ECO:0000269|PubMed:28152596"
FT DISULFID 56..62
FT /evidence="ECO:0000269|PubMed:28152596"
SQ SEQUENCE 63 AA; 7085 MW; F0518D7FB85ACFE1 CRC64;
MGKLTILVLV AAILLSTQVM VQGDRDQPAD RNAVPRDVNP GRARRKLMKV LRESECPWKP
WCG
