ID   COW4_CONAO              Reviewed;          10 AA.
AC   P0DPQ1;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2018, sequence version 1.
DT   23-FEB-2022, entry version 7.
DE   RecName: Full=Contryphan-Ar4 {ECO:0000305};
DE   AltName: Full=Ar1313 {ECO:0000303|PubMed:28152596};
OS   Conus araneosus (Cobweb cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Conus.
OX   NCBI_TaxID=101286;
RN   [1]
RP   PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, MASS SPECTROMETRY,
RP   SUBCELLULAR LOCATION, DISULFIDE BOND, D-AMINO ACID AT TRP-6, AMIDATION AT
RP   CYS-10, AND GAMMA-CARBOXYGLUTAMATION AT GLU-3.
RC   TISSUE=Venom;
RX   PubMed=28152596; DOI=10.1021/acs.jproteome.6b00776;
RA   Vijayasarathy M., Basheer S.M., Franklin J.B., Balaram P.;
RT   "Contryphan genes and mature peptides in the venom of nine cone snail
RT   species by transcriptomic and mass spectrometric analysis.";
RL   J. Proteome Res. 16:763-772(2017).
CC   -!- FUNCTION: Its target is unknown, but this toxin may modulate voltage-
CC       activated calcium channels (Cav) or calcium-dependent potassium
CC       channels (KCa). {ECO:0000250|UniProtKB:P0C248,
CC       ECO:0000250|UniProtKB:P0C250, ECO:0000250|UniProtKB:P62903,
CC       ECO:0000250|UniProtKB:P83047}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28152596}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:28152596}.
CC   -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1313.56; Method=MALDI; Note=Monoisotopic mass,
CC       Ar1313 (gamma-carboxylated).; Evidence={ECO:0000269|PubMed:28152596};
CC   -!- SIMILARITY: Belongs to the O2 superfamily. Contryphan family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011062; Contryphan_CS.
DR   PROSITE; PS60027; CONTRYPHAN; 1.
PE   1: Evidence at protein level;
KW   Amidation; D-amino acid; Direct protein sequencing; Disulfide bond;
KW   Gamma-carboxyglutamic acid; Ion channel impairing toxin; Neurotoxin;
KW   Secreted; Toxin.
FT   PEPTIDE         1..10
FT                   /note="Contryphan-Ar4"
FT                   /evidence="ECO:0000269|PubMed:28152596"
FT                   /id="PRO_0000445128"
FT   MOD_RES         3
FT                   /note="4-carboxyglutamate"
FT                   /evidence="ECO:0000305|PubMed:28152596"
FT   MOD_RES         6
FT                   /note="D-tryptophan"
FT                   /evidence="ECO:0000305|PubMed:28152596"
FT   MOD_RES         10
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000269|PubMed:28152596"
FT   DISULFID        4..10
FT                   /evidence="ECO:0000269|PubMed:28152596"
SQ   SEQUENCE   10 AA;  1273 MW;  69297661F3676EBB CRC64;
     ESECPWHPWC