ID   COW_CONST               Reviewed;          63 AA.
AC   P0C837;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Contryphan-S {ECO:0000303|PubMed:16183187};
DE   AltName: Full=St990 {ECO:0000303|PubMed:17902199};
DE   Contains:
DE     RecName: Full=Contryphan-S St933 {ECO:0000303|PubMed:17902199};
DE   Flags: Precursor;
OS   Conus striatus (Striated cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Pionoconus.
OX   NCBI_TaxID=6493;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=16183187; DOI=10.1016/j.biochi.2005.08.001;
RA   Pi C., Liu Y., Peng C., Jiang X., Liu J., Xu B., Yu X., Yu Y., Jiang X.,
RA   Wang L., Dong M., Chen S., Xu A.-L.;
RT   "Analysis of expressed sequence tags from the venom ducts of Conus
RT   striatus: focusing on the expression profile of conotoxins.";
RL   Biochimie 88:131-140(2006).
RN   [2]
RP   PROTEIN SEQUENCE OF 55-62 AND 56-62, IDENTIFICATION BY MASS SPECTROMETRY,
RP   MASS SPECTROMETRY, SUBCELLULAR LOCATION, HYDROXYLATION AT PRO-57, D-AMINO
RP   ACID AT TRP-58, AND AMIDATION AT CYS-62.
RC   TISSUE=Venom;
RX   PubMed=17902199; DOI=10.1002/rcm.3225;
RA   Thakur S.S., Balaram P.;
RT   "Rapid mass spectral identification of contryphans. Detection of
RT   characteristic peptide ions by fragmentation of intact disulfide-bonded
RT   peptides in crude venom.";
RL   Rapid Commun. Mass Spectrom. 21:3420-3426(2007).
CC   -!- FUNCTION: Its target is unknown, but this toxin may modulate voltage-
CC       activated calcium channels (Cav) or calcium-dependent potassium
CC       channels (KCa). {ECO:0000250|UniProtKB:P0C248,
CC       ECO:0000250|UniProtKB:P0C250, ECO:0000250|UniProtKB:P62903,
CC       ECO:0000250|UniProtKB:P83047}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17902199}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:17902199}.
CC   -!- DOMAIN: The cysteine framework is C-C. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: [Contryphan-S]: Mass=990; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17902199};
CC   -!- MASS SPECTROMETRY: [Contryphan-S St933]: Mass=933; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17902199};
CC   -!- MISCELLANEOUS: Exists in two forms, due to cis-trans isomerization at
CC       56-Cys-hydroxyPro-57. The cis conformation is the major form.
CC       {ECO:0000250|UniProtKB:P58787}.
CC   -!- SIMILARITY: Belongs to the O2 superfamily. Contryphan family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0C837; -.
DR   ConoServer; 3551; Bromocontryphan-S.
DR   ConoServer; 3548; Contryphan-S precursor.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004214; Conotoxin.
DR   InterPro; IPR011062; Contryphan_CS.
DR   Pfam; PF02950; Conotoxin; 1.
DR   PROSITE; PS60027; CONTRYPHAN; 1.
PE   1: Evidence at protein level;
KW   Amidation; D-amino acid; Direct protein sequencing; Disulfide bond;
KW   Hydroxylation; Ion channel impairing toxin; Neurotoxin; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..54
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000345106"
FT   PEPTIDE         55..62
FT                   /note="Contryphan-S"
FT                   /evidence="ECO:0000269|PubMed:17902199"
FT                   /id="PRO_0000345107"
FT   PEPTIDE         56..62
FT                   /note="Contryphan-S St933"
FT                   /evidence="ECO:0000269|PubMed:17902199"
FT                   /id="PRO_0000439695"
FT   REGION          23..49
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         57
FT                   /note="4-hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:17902199"
FT   MOD_RES         58
FT                   /note="D-tryptophan"
FT                   /evidence="ECO:0000269|PubMed:17902199"
FT   MOD_RES         62
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000269|PubMed:17902199"
FT   DISULFID        56..62
FT                   /evidence="ECO:0000305|PubMed:17902199"
SQ   SEQUENCE   63 AA;  6831 MW;  9D4852D500CAB05A CRC64;
     MEKLTILVLV AAVLLSTQVM VQGDADQPAD RDAVPRDDNA GGTDGKFMNV QRRSGCPWEP
     WCG