ID   2B_CMVQ                 Reviewed;         100 AA.
AC   Q66125;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 57.
DE   RecName: Full=Suppressor of silencing 2b;
DE   AltName: Full=Protein 2b;
GN   ORFNames=ORF2b;
OS   Cucumber mosaic virus (strain Q) (CMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=12310;
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8291242; DOI=10.1006/viro.1994.1071;
RA   Ding S.W., Anderson B.J., Haase H.R., Symons R.H.;
RT   "New overlapping gene encoded by the cucumber mosaic virus genome.";
RL   Virology 198:593-601(1994).
RN   [2]
RP   RETRACTED PAPER.
RX   PubMed=9822616; DOI=10.1093/emboj/17.22.6739;
RA   Brigneti G., Voinnet O., Li W.X., Ji L.H., Ding S.W., Baulcombe D.C.;
RT   "Viral pathogenicity determinants are suppressors of transgene silencing in
RT   Nicotiana benthamiana.";
RL   EMBO J. 17:6739-6746(1998).
RN   [3]
RP   SUBCELLULAR LOCATION, AND MUTAGENESIS OF 23-ARG--ARG-25.
RX   PubMed=10747034; DOI=10.1093/emboj/19.7.1672;
RA   Lucy A.P., Guo H.S., Li W.X., Ding S.W.;
RT   "Suppression of post-transcriptional gene silencing by a plant viral
RT   protein localized in the nucleus.";
RL   EMBO J. 19:1672-1680(2000).
RN   [4]
RP   FUNCTION.
RX   PubMed=11823432; DOI=10.1093/emboj/21.3.398;
RA   Guo H.S., Ding S.W.;
RT   "A viral protein inhibits the long range signaling activity of the gene
RT   silencing signal.";
RL   EMBO J. 21:398-407(2002).
RN   [5]
RP   RETRACTION NOTICE OF PUBMED:9822616.
RX   PubMed=26286615; DOI=10.15252/embj.201570030;
RA   Brigneti G., Voinnet O., Li W.X., Ji L.H., Ding S.W., Baulcombe D.C.;
RL   EMBO J. 34:2595-2595(2015).
CC   -!- FUNCTION: Acts as suppressor of RNA-mediated gene silencing, also known
CC       as post-transcriptional gene silencing (PTGS), a mechanism of plant
CC       viral defense that limits the accumulation of viral RNAs. May directly
CC       interfere with the mobile silencing signal.
CC       {ECO:0000269|PubMed:11823432}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:10747034}.
CC   -!- SIMILARITY: Belongs to the cucumovirus/ilarvirus protein 2b family.
CC       {ECO:0000305}.
CC   -!- CAUTION: A paper showing a role as suppressor of RNA-mediated gene
CC       silencing has been retracted because of duplications in figures 5B and
CC       6I of this paper. {ECO:0000305|PubMed:26286615}.
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DR   EMBL; Z21863; CAA79918.1; -; Genomic_RNA.
DR   PIR; B49598; B49598.
DR   SMR; Q66125; -.
DR   ELM; Q66125; -.
DR   Proteomes; UP000008454; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004946; Cucumo_2B.
DR   Pfam; PF03263; Cucumo_2B; 1.
PE   1: Evidence at protein level;
KW   Host nucleus; Reference proteome; Suppressor of RNA silencing.
FT   CHAIN           1..100
FT                   /note="Suppressor of silencing 2b"
FT                   /id="PRO_0000083284"
FT   MOTIF           22..27
FT                   /note="Nuclear localization signal"
FT   MUTAGEN         23..25
FT                   /note="RRR->QAQ: 70% loss of nuclear localization."
FT                   /evidence="ECO:0000269|PubMed:10747034"
SQ   SEQUENCE   100 AA;  11580 MW;  90838CD2411D6552 CRC64;
     MDVLTVVVST ADLHLANLQE VKRRRRRSHV RNRRARGYKS PSERARSIAR LFQMLPFHGV
     DPVDWFPDVV RSPSVTSLVS YESFDDTDWF AGNEWAEGSF