COX12_NEUCR
ID COX12_NEUCR Reviewed; 84 AA.
AC Q1K8U2;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=Cytochrome c oxidase subunit 12, mitochondrial;
DE AltName: Full=Cytochrome c oxidase polypeptide VIb;
DE AltName: Full=Cytochrome c oxidase subunit Cox6b {ECO:0000303|PubMed:31316820};
GN Name=cox-13; ORFNames=NCU06741;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [2]
RP COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
RX PubMed=17873079; DOI=10.1128/ec.00149-07;
RA Marques I., Dencher N.A., Videira A., Krause F.;
RT "Supramolecular organization of the respiratory chain in Neurospora crassa
RT mitochondria.";
RL Eukaryot. Cell 6:2391-2405(2007).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.5 ANGSTROMS), AND SUBUNIT.
RX PubMed=31316820; DOI=10.1107/s2052252519007486;
RA Bausewein T., Nussberger S., Kuehlbrandt W.;
RT "Cryo-EM structure of Neurospora crassa respiratory complex IV.";
RL IUCrJ 6:773-780(2019).
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of Cox2 and heme A of Cox1 to the
CC active site in Cox1, a binuclear center (BNC) formed by heme A3 and
CC copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules
CC using 4 electrons from cytochrome c in the IMS and 4 protons from the
CC mitochondrial matrix. {ECO:0000250|UniProtKB:Q01519}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000250|UniProtKB:Q01519}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 11 subunits. The complex is composed of
CC a catalytic core of 3 subunits Cox1, Cox2 and Cox3, encoded in the
CC mitochondrial DNA, and 8 supernumerary subunits Cox4, Cox5a/Cox5, Cox6,
CC Cox7, Cox8, Cox7a/Cox9, Cox6b/Cox12 and Cox6a/Cox13, which are encoded
CC in the nuclear genome (PubMed:31316820). The complex exists as a
CC monomer or a dimer and forms respiratory supercomplexes (SCs) in the
CC inner mitochondrial membrane with NADH-ubiquinone oxidoreductase
CC (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome
CC b-c1 complex, complex III, CIII), resulting in various different
CC assemblies (supercomplexes I(1)IV(1), I(1)III(3)IV(2), III(2)IV(1) and
CC III(2)IV(2) as well as larger supercomplexes of compositions like
CC I(1)III(2)IV(5-6)) (PubMed:17873079). {ECO:0000269|PubMed:17873079,
CC ECO:0000269|PubMed:31316820}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:31316820}; Peripheral membrane protein
CC {ECO:0000269|PubMed:31316820}; Intermembrane side
CC {ECO:0000269|PubMed:31316820}.
CC -!- DOMAIN: The Cx9C/Cx10C motifs are involved in the recognition by the
CC mitochondrial MIA40-ERV1 disulfide relay system and the subsequent
CC transfer of disulfide bonds by dithiol/disulfide exchange reactions to
CC the newly imported protein. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 6B family.
CC {ECO:0000305}.
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DR EMBL; CM002237; EAA34277.1; -; Genomic_DNA.
DR RefSeq; XP_963513.1; XM_958420.3.
DR AlphaFoldDB; Q1K8U2; -.
DR SMR; Q1K8U2; -.
DR STRING; 5141.EFNCRP00000006886; -.
DR EnsemblFungi; EAA34277; EAA34277; NCU06741.
DR GeneID; 23568465; -.
DR KEGG; ncr:NCU06741; -.
DR VEuPathDB; FungiDB:NCU06741; -.
DR HOGENOM; CLU_133964_1_2_1; -.
DR InParanoid; Q1K8U2; -.
DR OMA; CPNEWIA; -.
DR UniPathway; UPA00705; -.
DR Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:EnsemblFungi.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IEA:EnsemblFungi.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:EnsemblFungi.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IEA:EnsemblFungi.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IEA:EnsemblFungi.
DR CDD; cd00926; Cyt_c_Oxidase_VIb; 1.
DR Gene3D; 1.10.10.140; -; 1.
DR InterPro; IPR003213; Cyt_c_oxidase_su6B.
DR InterPro; IPR036549; Cyt_c_oxidase_su6B_sf.
DR Pfam; PF02297; COX6B; 1.
DR PIRSF; PIRSF000278; Cyt_c_oxidase_6B; 1.
DR SUPFAM; SSF47694; SSF47694; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome.
FT CHAIN 1..84
FT /note="Cytochrome c oxidase subunit 12, mitochondrial"
FT /id="PRO_0000448902"
FT DOMAIN 27..70
FT /note="CHCH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 30..40
FT /note="Cx9C motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 51..62
FT /note="Cx10C motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 30..62
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 40..51
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
SQ SEQUENCE 84 AA; 10050 MW; 9791227688D07C5F CRC64;
MSDDERVTKP FKFVTGVDAR FPNVNQTKHC WQNYVDYHKC ILAKGEDFAP CRQFWLAYRS
LCPSGWYQRW DEQREAGNFP VKLE
