COX18_YEAST
ID COX18_YEAST Reviewed; 316 AA.
AC P53239; D6VUJ7;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Cytochrome c oxidase assembly protein COX18, mitochondrial;
DE AltName: Full=Cytochrome c oxidase assembly protein 18;
DE Flags: Precursor;
GN Name=COX18; OrderedLocusNames=YGR062C; ORFNames=G4532;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RX PubMed=10809734; DOI=10.1074/jbc.275.20.14898;
RA Souza R.L., Green-Willms N.S., Fox T.D., Tzagoloff A., Nobrega F.G.;
RT "Cloning and characterization of COX18, a Saccharomyces cerevisiae PET gene
RT required for the assembly of cytochrome oxidase.";
RL J. Biol. Chem. 275:14898-14902(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND INTERACTION WITH PNT1 AND MSS2.
RX PubMed=11950926; DOI=10.1091/mbc.01-12-0580;
RA Saracco S.A., Fox T.D.;
RT "Cox18p is required for export of the mitochondrially encoded Saccharomyces
RT cerevisiae Cox2p C-tail and interacts with Pnt1p and Mss2p in the inner
RT membrane.";
RL Mol. Biol. Cell 13:1122-1131(2002).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for the insertion of integral membrane proteins into
CC the mitochondrial inner membrane. Essential for the activity and
CC assembly of cytochrome c oxidase. Plays a central role in the
CC translocation and export of the C-terminal part of the COX2 protein
CC into the mitochondrial intermembrane space.
CC {ECO:0000269|PubMed:11950926}.
CC -!- SUBUNIT: Interacts with PNT1 and MSS2. {ECO:0000269|PubMed:11950926}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:10809734}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:10809734}.
CC -!- MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the OXA1/ALB3/YidC family. {ECO:0000305}.
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DR EMBL; U59742; AAB03186.1; -; Genomic_DNA.
DR EMBL; Z72847; CAA97064.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08158.1; -; Genomic_DNA.
DR PIR; S64357; S64357.
DR RefSeq; NP_011576.1; NM_001181191.1.
DR AlphaFoldDB; P53239; -.
DR BioGRID; 33307; 102.
DR DIP; DIP-5161N; -.
DR IntAct; P53239; 3.
DR MINT; P53239; -.
DR STRING; 4932.YGR062C; -.
DR TCDB; 2.A.9.4.1; the membrane protein insertase (yidc/alb3/oxa1) family.
DR MaxQB; P53239; -.
DR PaxDb; P53239; -.
DR PRIDE; P53239; -.
DR EnsemblFungi; YGR062C_mRNA; YGR062C; YGR062C.
DR GeneID; 852953; -.
DR KEGG; sce:YGR062C; -.
DR SGD; S000003294; COX18.
DR VEuPathDB; FungiDB:YGR062C; -.
DR eggNOG; KOG1239; Eukaryota.
DR GeneTree; ENSGT00530000063506; -.
DR HOGENOM; CLU_029282_2_1_1; -.
DR InParanoid; P53239; -.
DR OMA; EASHIPW; -.
DR BioCyc; YEAST:G3O-30777-MON; -.
DR PRO; PR:P53239; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53239; protein.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0032977; F:membrane insertase activity; IMP:SGD.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IBA:GO_Central.
DR GO; GO:0051205; P:protein insertion into membrane; IBA:GO_Central.
DR GO; GO:0032979; P:protein insertion into mitochondrial inner membrane from matrix; IMP:SGD.
DR InterPro; IPR001708; YidC/ALB3/OXA1/COX18.
DR PANTHER; PTHR12428; PTHR12428; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..316
FT /note="Cytochrome c oxidase assembly protein COX18,
FT mitochondrial"
FT /id="PRO_0000020357"
FT TOPO_DOM ?..163
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000305"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..213
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000305"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..274
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000305"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 296..316
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000305"
SQ SEQUENCE 316 AA; 35650 MW; EBC5B42722B46687 CRC64;
MLKRLANRQN GFASFSCSSV GLRYGRTNPS TKRSFSLFQS VADTFLTVHE ASHIPWIVLV
PLTTMTLRTL VTLPFSIWQR RRILKQQELR KLVQPITPII KLRLAAVTNK KSRNAARISS
NGSFMPLQLQ NAGVLTPEQI TLLAVKETRK RQKKLFKKYN VPLWKNALLP MVQIPLWVTV
SMGIRTLTET QLIESFYPSW FSALGFSSFD LSSPLVAMPL LAPILVGTLA VLNVELNGRL
MFSSSLSSQG IKTISRNSTR VQEAMTSILN VSRLGCVVML AMSSQAPFLL SLYWISSQLF
SLVQNIILNW IYPYQR
