COX3_DROME
ID COX3_DROME Reviewed; 262 AA.
AC P00417; B1PTQ2; Q7HMA0; Q9MGN4;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Cytochrome c oxidase subunit 3;
DE EC=7.1.1.9;
DE AltName: Full=Cytochrome c oxidase polypeptide III;
GN Name=mt:CoIII; Synonyms=CoIII;
OS Drosophila melanogaster (Fruit fly).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT MET-170.
RC STRAIN=Oregon-R, and Zimbabwe 53;
RX PubMed=10903372; DOI=10.1007/s002390010066;
RA Ballard J.W.O.;
RT "Comparative genomics of mitochondrial DNA in members of the Drosophila
RT melanogaster subgroup.";
RL J. Mol. Evol. 51:48-63(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Paris;
RX PubMed=11298822; DOI=10.1046/j.1365-2540.2001.00814.x;
RA Azou Y., Bregliano J.C.;
RT "I-R system of hybrid dysgenesis in Drosophila melanogaster: analysis of
RT the mitochondrial DNA in reactive strains exhibiting different potentials
RT for I factor transposition.";
RL Heredity 86:110-116(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=18296276; DOI=10.1098/rsbl.2007.0575;
RA O'Grady P.M., DeSalle R.;
RT "Out of Hawaii: the origin and biogeography of the genus Scaptomyza
RT (Diptera: Drosophilidae).";
RL Biol. Lett. 4:195-199(2008).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8825764; DOI=10.1111/j.1365-2583.1995.tb00032.x;
RA Lewis D.L., Farr C.L., Kaguni L.S.;
RT "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide
RT sequence and evolutionary comparisons.";
RL Insect Mol. Biol. 4:263-278(1995).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RA Wan K., Celniker S.;
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-179.
RC STRAIN=Oregon-R;
RX PubMed=6408489; DOI=10.1038/304234a0;
RA de Bruijn M.H.L.;
RT "Drosophila melanogaster mitochondrial DNA, a novel organization and
RT genetic code.";
RL Nature 304:234-241(1983).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-262.
RX PubMed=6304652; DOI=10.1093/nar/11.8.2411;
RA Clary D.O., Wahleithner J.A., Wolstenholme D.R.;
RT "Transfer RNA genes in Drosophila mitochondrial DNA: related 5' flanking
RT sequences and comparisons to mammalian mitochondrial tRNA genes.";
RL Nucleic Acids Res. 11:2411-2425(1983).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-262.
RC STRAIN=Bretagne;
RX PubMed=3130291; DOI=10.1093/genetics/118.4.649;
RA Garesse R.;
RT "Drosophila melanogaster mitochondrial DNA: gene organization and
RT evolutionary considerations.";
RL Genetics 118:649-663(1988).
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules using 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P00420}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-
CC [cytochrome c] + 4 H(+)(out) + 2 H2O; Xref=Rhea:RHEA:11436,
CC Rhea:RHEA-COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:29033,
CC ChEBI:CHEBI:29034; EC=7.1.1.9;
CC Evidence={ECO:0000250|UniProtKB:P00420};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11437;
CC Evidence={ECO:0000250|UniProtKB:P00420};
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of a catalytic core of 3 subunits and
CC several supernumerary subunits. The complex exists as a monomer or a
CC dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1
CC complex, complex III, CIII). {ECO:0000250|UniProtKB:P00420}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P00420}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P00420}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 3 family.
CC {ECO:0000305}.
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DR EMBL; AF200828; AAF77231.1; -; Genomic_DNA.
DR EMBL; AF200829; AAF77243.1; -; Genomic_DNA.
DR EMBL; AJ400907; CAB91056.1; -; Genomic_DNA.
DR EMBL; EU493887; ACA62455.1; -; Genomic_DNA.
DR EMBL; KJ947872; AIC64009.1; -; Genomic_DNA.
DR EMBL; U37541; AAC47816.1; -; Genomic_DNA.
DR EMBL; J01404; AAB59243.1; -; Genomic_DNA.
DR EMBL; M37275; AAA69708.1; -; Genomic_DNA.
DR PIR; A00485; OTFF3.
DR RefSeq; YP_009047271.1; NC_024511.2.
DR AlphaFoldDB; P00417; -.
DR SMR; P00417; -.
DR STRING; 7227.FBpp0100180; -.
DR PaxDb; P00417; -.
DR EnsemblMetazoa; FBtr0100868; FBpp0100180; FBgn0013676.
DR GeneID; 19893540; -.
DR KEGG; dme:Dmel_CG34074; -.
DR CTD; 4514; -.
DR FlyBase; FBgn0013676; mt:CoIII.
DR VEuPathDB; VectorBase:FBgn0013676; -.
DR eggNOG; KOG4664; Eukaryota.
DR GeneTree; ENSGT00390000013064; -.
DR HOGENOM; CLU_044071_0_0_1; -.
DR InParanoid; P00417; -.
DR OMA; SIYWWGS; -.
DR OrthoDB; 1304563at2759; -.
DR PhylomeDB; P00417; -.
DR Reactome; R-DME-5628897; TP53 Regulates Metabolic Genes.
DR Reactome; R-DME-611105; Respiratory electron transport.
DR Reactome; R-DME-9707564; Cytoprotection by HMOX1.
DR BioGRID-ORCS; 19893540; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 19893540; -.
DR PRO; PR:P00417; -.
DR Proteomes; UP000000803; Mitochondrion.
DR Bgee; FBgn0013676; Expressed in brain and 11 other tissues.
DR ExpressionAtlas; P00417; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0009055; F:electron transfer activity; IBA:GO_Central.
DR GO; GO:0015453; F:oxidoreduction-driven active transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central.
DR CDD; cd01665; Cyt_c_Oxidase_III; 1.
DR Gene3D; 1.20.120.80; -; 1.
DR InterPro; IPR024791; Cyt_c/ubiquinol_Oxase_su3.
DR InterPro; IPR033945; Cyt_c_oxase_su3_dom.
DR InterPro; IPR000298; Cyt_c_oxidase-like_su3.
DR InterPro; IPR035973; Cyt_c_oxidase_su3-like_sf.
DR InterPro; IPR013833; Cyt_c_oxidase_su3_a-hlx.
DR PANTHER; PTHR11403; PTHR11403; 1.
DR Pfam; PF00510; COX3; 1.
DR SUPFAM; SSF81452; SSF81452; 1.
DR PROSITE; PS50253; COX3; 1.
PE 3: Inferred from homology;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Translocase; Transmembrane; Transmembrane helix.
FT CHAIN 1..262
FT /note="Cytochrome c oxidase subunit 3"
FT /id="PRO_0000183767"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 170
FT /note="L -> M (in strain: Zimbabwe 53)"
FT /evidence="ECO:0000269|PubMed:10903372"
SQ SEQUENCE 262 AA; 30064 MW; 68D5AEFC2297C130 CRC64;
MSTHSNHPFH LVDYSPWPLT GAIGAMTTVS GMVKWFHQYD ISLFVLGNII TILTVYQWWR
DVSREGTYQG LHTYAVTIGL RWGMILFILS EVLFFVSFFW AFFHSSLSPA IELGASWPPM
GIISFNPFQI PLLNTAILLA SGVTVTWAHH SLMENNHSQT TQGLFFTVLL GIYFTILQAY
EYIEAPFTIA DSIYGSTFFM ATGFHGIHVL IGTTFLLVCL LRHLNNHFSK NHHFGFEAAA
WYWHFVDVVW LFLYITIYWW GG
