COX6C_BOVIN
ID COX6C_BOVIN Reviewed; 74 AA.
AC P04038; A0JNM4;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Cytochrome c oxidase subunit 6C;
DE AltName: Full=Cytochrome c oxidase polypeptide VIc;
DE AltName: Full=Cytochrome c oxidase subunit STA;
GN Name=COX6C;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal medulla;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 2-74.
RC TISSUE=Liver;
RX PubMed=2988583; DOI=10.1515/bchm3.1985.366.1.257;
RA Erdweg M., Buse G.;
RT "Studies on cytochrome c oxidase, XI. The amino-acid sequence of bovine
RT heart polypeptide VIc.";
RL Biol. Chem. Hoppe-Seyler 366:257-263(1985).
RN [3]
RP PROTEIN SEQUENCE OF 2-21.
RC TISSUE=Liver;
RX PubMed=2844245; DOI=10.1021/bi00413a048;
RA Yanamura W., Zhang Y.-Z., Takamiya S., Capaldi R.A.;
RT "Tissue-specific differences between heart and liver cytochrome c
RT oxidase.";
RL Biochemistry 27:4909-4914(1988).
RN [4]
RP SUBUNIT.
RX PubMed=26698328; DOI=10.1074/jbc.m115.680553;
RA Shinzawa-Itoh K., Shimomura H., Yanagisawa S., Shimada S., Takahashi R.,
RA Oosaki M., Ogura T., Tsukihara T.;
RT "Purification of active respiratory supercomplex from bovine heart
RT mitochondria enables functional studies.";
RL J. Biol. Chem. 291:4178-4184(2016).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RX PubMed=8638158; DOI=10.1126/science.272.5265.1136;
RA Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H.,
RA Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.;
RT "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8
RT A.";
RL Science 272:1136-1144(1996).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RC TISSUE=Heart;
RX PubMed=10089392; DOI=10.1107/s0907444998006362;
RA Tomizaki T., Yamashita E., Yamaguchi H., Aoyama H., Tsukihara T.,
RA Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.;
RT "Structure analysis of bovine heart cytochrome c oxidase at 2.8 A
RT resolution.";
RL Acta Crystallogr. D 55:31-45(1999).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
RC TISSUE=Heart;
RX PubMed=10771420; DOI=10.1107/s0907444900002213;
RA Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T.,
RA Shinzawa-Itoh K., Nakashima R., Yoshikawa S.;
RT "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from
RT bovine heart at 2.9 A resolution.";
RL Acta Crystallogr. D 56:529-535(2000).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
RX PubMed=20385840; DOI=10.1073/pnas.0910410107;
RA Muramoto K., Ohta K., Shinzawa-Itoh K., Kanda K., Taniguchi M.,
RA Nabekura H., Yamashita E., Tsukihara T., Yoshikawa S.;
RT "Bovine cytochrome c oxidase structures enable O2 reduction with
RT minimization of reactive oxygens and provide a proton-pumping gate.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:7740-7745(2010).
RN [9]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.10 ANGSTROMS).
RX PubMed=27830641; DOI=10.7554/elife.21290;
RA Sousa J.S., Mills D.J., Vonck J., Kuehlbrandt W.;
RT "Functional asymmetry and electron flow in the bovine respirasome.";
RL Elife 5:0-0(2016).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS).
RX PubMed=27605664; DOI=10.1074/jbc.m115.711770;
RA Yano N., Muramoto K., Shimada A., Takemura S., Baba J., Fujisawa H.,
RA Mochizuki M., Shinzawa-Itoh K., Yamashita E., Tsukihara T., Yoshikawa S.;
RT "The Mg2+-containing water cluster of mammalian cytochrome c oxidase
RT collects four pumping proton equivalents in each catalytic cycle.";
RL J. Biol. Chem. 291:23882-23894(2016).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
RX PubMed=31533957; DOI=10.1073/pnas.1907183116;
RA Shinzawa-Itoh K., Sugimura T., Misaki T., Tadehara Y., Yamamoto S.,
RA Hanada M., Yano N., Nakagawa T., Uene S., Yamada T., Aoyama H.,
RA Yamashita E., Tsukihara T., Yoshikawa S., Muramoto K.;
RT "Monomeric structure of an active form of bovine cytochrome c oxidase.";
RL Proc. Natl. Acad. Sci. U.S.A. 116:19945-19951(2019).
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules using 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000269|PubMed:27605664}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000305|PubMed:27605664}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 14 subunits. The complex is composed of
CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC the mitochondrial DNA, and 11 supernumerary subunits COX4I1 (or
CC COX4I2), COX5A, COX5B, COX6A2 (or COX6A1), COX6B1 (or COX6B2), COX6C,
CC COX7A1 (or COX7A2), COX7B, COX7C, COX8B and NDUFA4, which are encoded
CC in the nuclear genome (PubMed:8638158). The complex exists as a monomer
CC or a dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC III, CIII), resulting in different assemblies (supercomplex
CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)) (PubMed:26698328,
CC PubMed:27830641). {ECO:0000269|PubMed:26698328,
CC ECO:0000269|PubMed:27830641, ECO:0000269|PubMed:8638158}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:27605664, ECO:0000269|PubMed:31533957}; Single-pass
CC membrane protein {ECO:0000269|PubMed:27605664,
CC ECO:0000269|PubMed:31533957}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 6c family.
CC {ECO:0000305}.
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DR EMBL; BC126788; AAI26789.1; -; mRNA.
DR PIR; A00496; OGBO6C.
DR RefSeq; NP_001231040.1; NM_001244111.1.
DR PDB; 1OCC; X-ray; 2.80 A; I/V=2-74.
DR PDB; 1OCO; X-ray; 2.80 A; I/V=2-74.
DR PDB; 1OCR; X-ray; 2.35 A; I/V=2-74.
DR PDB; 1OCZ; X-ray; 2.90 A; I/V=2-74.
DR PDB; 1V54; X-ray; 1.80 A; I/V=2-74.
DR PDB; 1V55; X-ray; 1.90 A; I/V=2-74.
DR PDB; 2DYR; X-ray; 1.80 A; I/V=2-74.
DR PDB; 2DYS; X-ray; 2.20 A; I/V=2-74.
DR PDB; 2EIJ; X-ray; 1.90 A; I/V=2-74.
DR PDB; 2EIK; X-ray; 2.10 A; I/V=2-74.
DR PDB; 2EIL; X-ray; 2.10 A; I/V=2-74.
DR PDB; 2EIM; X-ray; 2.60 A; I/V=2-74.
DR PDB; 2EIN; X-ray; 2.70 A; I/V=2-74.
DR PDB; 2OCC; X-ray; 2.30 A; I/V=2-74.
DR PDB; 2Y69; X-ray; 1.95 A; I/V=1-74.
DR PDB; 2YBB; EM; 19.00 A; T=2-74.
DR PDB; 2ZXW; X-ray; 2.50 A; I/V=2-74.
DR PDB; 3ABK; X-ray; 2.00 A; I/V=2-74.
DR PDB; 3ABL; X-ray; 2.10 A; I/V=2-74.
DR PDB; 3ABM; X-ray; 1.95 A; I/V=2-74.
DR PDB; 3AG1; X-ray; 2.20 A; I/V=2-74.
DR PDB; 3AG2; X-ray; 1.80 A; I/V=2-74.
DR PDB; 3AG3; X-ray; 1.80 A; I/V=2-74.
DR PDB; 3AG4; X-ray; 2.05 A; I/V=2-74.
DR PDB; 3ASN; X-ray; 3.00 A; I/V=2-74.
DR PDB; 3ASO; X-ray; 2.30 A; I/V=2-74.
DR PDB; 3WG7; X-ray; 1.90 A; I/V=2-74.
DR PDB; 3X2Q; X-ray; 2.00 A; I/V=2-74.
DR PDB; 5B1A; X-ray; 1.50 A; I/V=2-74.
DR PDB; 5B1B; X-ray; 1.60 A; I/V=2-74.
DR PDB; 5B3S; X-ray; 1.68 A; I/V=3-74.
DR PDB; 5GPN; EM; 5.40 A; 6=2-74.
DR PDB; 5IY5; X-ray; 2.00 A; I/V=2-74.
DR PDB; 5LUF; EM; 9.10 A; 6=2-74.
DR PDB; 5W97; X-ray; 2.30 A; I/i=2-74.
DR PDB; 5WAU; X-ray; 1.95 A; I/i=2-74.
DR PDB; 5X19; X-ray; 2.20 A; I/V=2-74.
DR PDB; 5X1B; X-ray; 2.40 A; I/V=2-74.
DR PDB; 5X1F; X-ray; 2.20 A; I/V=2-74.
DR PDB; 5XDQ; X-ray; 1.77 A; I/V=2-74.
DR PDB; 5XDX; X-ray; 1.99 A; I/V=2-74.
DR PDB; 5XTH; EM; 3.90 A; 5=2-74.
DR PDB; 5XTI; EM; 17.40 A; 5/B5=2-74.
DR PDB; 5Z84; X-ray; 1.85 A; I/V=2-74.
DR PDB; 5Z85; X-ray; 1.85 A; I/V=2-74.
DR PDB; 5Z86; X-ray; 1.85 A; I/V=2-74.
DR PDB; 5ZCO; X-ray; 1.90 A; I/V=2-74.
DR PDB; 5ZCP; X-ray; 1.65 A; I/V=2-74.
DR PDB; 5ZCQ; X-ray; 1.65 A; I/V=2-74.
DR PDB; 6J8M; X-ray; 1.90 A; I/V=2-74.
DR PDB; 6JUW; X-ray; 1.80 A; I/V=2-74.
DR PDB; 6JY3; X-ray; 1.85 A; I=2-74.
DR PDB; 6JY4; X-ray; 1.95 A; I=2-74.
DR PDB; 6NKN; X-ray; 2.50 A; I/V=2-74.
DR PDB; 6NMF; X-ray; 2.80 A; I/V=2-74.
DR PDB; 6NMP; X-ray; 2.90 A; I/V=2-74.
DR PDB; 7COH; X-ray; 1.30 A; I/V=2-74.
DR PDB; 7CP5; X-ray; 1.76 A; I/V=2-74.
DR PDB; 7D5W; X-ray; 1.84 A; I/V=2-74.
DR PDB; 7D5X; X-ray; 1.74 A; I/V=2-74.
DR PDB; 7EV7; X-ray; 1.70 A; I/V=2-74.
DR PDB; 7THU; X-ray; 1.93 A; IaI/VaV=2-74.
DR PDB; 7TIE; X-ray; 1.90 A; IaI/VaV=2-74.
DR PDB; 7TIH; X-ray; 2.35 A; IaI/VaV=2-74.
DR PDB; 7TII; X-ray; 2.45 A; IaI/VaV=2-74.
DR PDBsum; 1OCC; -.
DR PDBsum; 1OCO; -.
DR PDBsum; 1OCR; -.
DR PDBsum; 1OCZ; -.
DR PDBsum; 1V54; -.
DR PDBsum; 1V55; -.
DR PDBsum; 2DYR; -.
DR PDBsum; 2DYS; -.
DR PDBsum; 2EIJ; -.
DR PDBsum; 2EIK; -.
DR PDBsum; 2EIL; -.
DR PDBsum; 2EIM; -.
DR PDBsum; 2EIN; -.
DR PDBsum; 2OCC; -.
DR PDBsum; 2Y69; -.
DR PDBsum; 2YBB; -.
DR PDBsum; 2ZXW; -.
DR PDBsum; 3ABK; -.
DR PDBsum; 3ABL; -.
DR PDBsum; 3ABM; -.
DR PDBsum; 3AG1; -.
DR PDBsum; 3AG2; -.
DR PDBsum; 3AG3; -.
DR PDBsum; 3AG4; -.
DR PDBsum; 3ASN; -.
DR PDBsum; 3ASO; -.
DR PDBsum; 3WG7; -.
DR PDBsum; 3X2Q; -.
DR PDBsum; 5B1A; -.
DR PDBsum; 5B1B; -.
DR PDBsum; 5B3S; -.
DR PDBsum; 5GPN; -.
DR PDBsum; 5IY5; -.
DR PDBsum; 5LUF; -.
DR PDBsum; 5W97; -.
DR PDBsum; 5WAU; -.
DR PDBsum; 5X19; -.
DR PDBsum; 5X1B; -.
DR PDBsum; 5X1F; -.
DR PDBsum; 5XDQ; -.
DR PDBsum; 5XDX; -.
DR PDBsum; 5XTH; -.
DR PDBsum; 5XTI; -.
DR PDBsum; 5Z84; -.
DR PDBsum; 5Z85; -.
DR PDBsum; 5Z86; -.
DR PDBsum; 5ZCO; -.
DR PDBsum; 5ZCP; -.
DR PDBsum; 5ZCQ; -.
DR PDBsum; 6J8M; -.
DR PDBsum; 6JUW; -.
DR PDBsum; 6JY3; -.
DR PDBsum; 6JY4; -.
DR PDBsum; 6NKN; -.
DR PDBsum; 6NMF; -.
DR PDBsum; 6NMP; -.
DR PDBsum; 7COH; -.
DR PDBsum; 7CP5; -.
DR PDBsum; 7D5W; -.
DR PDBsum; 7D5X; -.
DR PDBsum; 7EV7; -.
DR PDBsum; 7THU; -.
DR PDBsum; 7TIE; -.
DR PDBsum; 7TIH; -.
DR PDBsum; 7TII; -.
DR AlphaFoldDB; P04038; -.
DR SMR; P04038; -.
DR CORUM; P04038; -.
DR DIP; DIP-38980N; -.
DR IntAct; P04038; 3.
DR STRING; 9913.ENSBTAP00000018778; -.
DR PaxDb; P04038; -.
DR PRIDE; P04038; -.
DR Ensembl; ENSBTAT00000018778; ENSBTAP00000018778; ENSBTAG00000014130.
DR Ensembl; ENSBTAT00000076438; ENSBTAP00000067188; ENSBTAG00000014130.
DR Ensembl; ENSBTAT00000081914; ENSBTAP00000065630; ENSBTAG00000014130.
DR GeneID; 614700; -.
DR KEGG; bta:614700; -.
DR VEuPathDB; HostDB:ENSBTAG00000014130; -.
DR eggNOG; ENOG502SEI2; Eukaryota.
DR GeneTree; ENSGT00940000163089; -.
DR HOGENOM; CLU_196254_0_0_1; -.
DR InParanoid; P04038; -.
DR OMA; FHIAGAF; -.
DR OrthoDB; 1618740at2759; -.
DR TreeFam; TF353619; -.
DR BRENDA; 7.1.1.9; 908.
DR UniPathway; UPA00705; -.
DR EvolutionaryTrace; P04038; -.
DR Proteomes; UP000009136; Chromosome 14.
DR Bgee; ENSBTAG00000014130; Expressed in tongue muscle and 104 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IEA:InterPro.
DR GO; GO:0045277; C:respiratory chain complex IV; IDA:UniProtKB.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IEA:InterPro.
DR CDD; cd00927; Cyt_c_Oxidase_VIc; 1.
DR Gene3D; 4.10.93.10; -; 1.
DR InterPro; IPR004204; COX6C.
DR InterPro; IPR034884; Cytochrome_c_oxidase_VIc/VIIs.
DR InterPro; IPR037169; Cytochrome_c_oxidase_VIc_sf.
DR Pfam; PF02937; COX6C; 1.
DR SUPFAM; SSF81415; SSF81415; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2844245,
FT ECO:0000269|PubMed:2988583"
FT CHAIN 2..74
FT /note="Cytochrome c oxidase subunit 6C"
FT /id="PRO_0000191301"
FT TOPO_DOM 2..12
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000269|PubMed:27605664"
FT TRANSMEM 13..53
FT /note="Helical"
FT /evidence="ECO:0000269|PubMed:27605664"
FT TOPO_DOM 54..74
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000269|PubMed:27605664"
FT HELIX 13..39
FT /evidence="ECO:0007829|PDB:7COH"
FT HELIX 41..53
FT /evidence="ECO:0007829|PDB:7COH"
FT HELIX 57..67
FT /evidence="ECO:0007829|PDB:7COH"
SQ SEQUENCE 74 AA; 8610 MW; E063BDB1B7AE9E4C CRC64;
MSTALAKPQM RGLLARRLRF HIVGAFMVSL GFATFYKFAV AEKRKKAYAD FYRNYDSMKD
FEEMRKAGIF QSAK
