COX8A_BOVIN
ID COX8A_BOVIN Reviewed; 69 AA.
AC P14622; Q32PC5;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Cytochrome c oxidase subunit 8A, mitochondrial;
DE AltName: Full=Cytochrome c oxidase polypeptide VIII-liver;
DE AltName: Full=Cytochrome c oxidase subunit 8-2;
DE AltName: Full=IX;
DE Flags: Precursor;
GN Name=COX8A; Synonyms=COX8, COX8L;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=1689292; DOI=10.1016/s0021-9258(19)39854-0;
RA Lightowlers R.N., Ewart G.D., Aggeler R.J., Zhang Y.-Z., Calavetta L.,
RA Capaldi R.A.;
RT "Isolation and characterization of the cDNAs encoding two isoforms of
RT subunit CIX of bovine cytochrome c oxidase.";
RL J. Biol. Chem. 265:2677-2681(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 26-69.
RC TISSUE=Liver;
RX PubMed=2844245; DOI=10.1021/bi00413a048;
RA Yanamura W., Zhang Y.-Z., Takamiya S., Capaldi R.A.;
RT "Tissue-specific differences between heart and liver cytochrome c
RT oxidase.";
RL Biochemistry 27:4909-4914(1988).
RN [4]
RP PROTEIN SEQUENCE OF 26-31.
RX PubMed=2176624; DOI=10.1016/0014-5793(90)80817-3;
RA Anthony G., Stroh A., Lottspeich F., Kadenbach B.;
RT "Different isozymes of cytochrome c oxidase are expressed in bovine smooth
RT muscle and skeletal or heart muscle.";
RL FEBS Lett. 277:97-100(1990).
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules using 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P10175}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 14 subunits. The complex is composed of
CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC the mitochondrial DNA, and 11 supernumerary subunits COX4I1 (or
CC COX4I2), COX5A, COX5B, COX6A2 (or COX6A1), COX6B1 (or COX6B2), COX6C,
CC COX7A1 (or COX7A2), COX7B, COX7C, COX8B and NDUFA4, which are encoded
CC in the nuclear genome (By similarity). The complex exists as a monomer
CC or a dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC III, CIII), resulting in different assemblies (supercomplex
CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)) (By similarity).
CC {ECO:0000250|UniProtKB:P10176}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P10176}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P10176}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase VIII family.
CC {ECO:0000305}.
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DR EMBL; J05201; AAA30467.1; -; mRNA.
DR EMBL; BC108170; AAI08171.1; -; mRNA.
DR PIR; B35537; B35537.
DR RefSeq; NP_776449.2; NM_174024.2.
DR AlphaFoldDB; P14622; -.
DR SMR; P14622; -.
DR CORUM; P14622; -.
DR PaxDb; P14622; -.
DR PRIDE; P14622; -.
DR Ensembl; ENSBTAT00000071400; ENSBTAP00000066680; ENSBTAG00000052272.
DR GeneID; 281091; -.
DR KEGG; bta:281091; -.
DR CTD; 1351; -.
DR VEuPathDB; HostDB:ENSBTAG00000052272; -.
DR eggNOG; ENOG502SA62; Eukaryota.
DR GeneTree; ENSGT00390000006255; -.
DR InParanoid; P14622; -.
DR OMA; MPPEQKL; -.
DR OrthoDB; 1632471at2759; -.
DR UniPathway; UPA00705; -.
DR Proteomes; UP000009136; Chromosome 29.
DR Bgee; ENSBTAG00000052272; Expressed in temporal cortex and 105 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IEA:InterPro.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0045277; C:respiratory chain complex IV; IBA:GO_Central.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IEA:InterPro.
DR Gene3D; 4.10.81.10; -; 1.
DR InterPro; IPR003205; Cyt_c_oxidase_su8.
DR InterPro; IPR036548; Cyt_c_oxidase_su8_sf.
DR PANTHER; PTHR16717; PTHR16717; 1.
DR Pfam; PF02285; COX8; 1.
DR SUPFAM; SSF81431; SSF81431; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..25
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:2176624,
FT ECO:0000269|PubMed:2844245"
FT CHAIN 26..69
FT /note="Cytochrome c oxidase subunit 8A, mitochondrial"
FT /id="PRO_0000006182"
FT TOPO_DOM 26..36
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:P10176"
FT TRANSMEM 37..60
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT TOPO_DOM 61..69
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:P10176"
FT CONFLICT 3
FT /note="V -> E (in Ref. 2; AAI08171)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="D -> I (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 50
FT /note="C -> D (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 69 AA; 7743 MW; 539BCFE4C945756B CRC64;
MSVLTPLLLR GLTGPARRLP VPRAQIHSKP PREQLGTMDI AIGLTSCFLC FLLPSGWVLS
HMENYKKRE
