COX8B_MOUSE
ID COX8B_MOUSE Reviewed; 70 AA.
AC P48772;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Cytochrome c oxidase subunit 8B, mitochondrial;
DE AltName: Full=Cytochrome c oxidase polypeptide VIII-heart;
DE AltName: Full=Cytochrome c oxidase subunit 8-1;
DE AltName: Full=Cytochrome c oxidase subunit 8H;
DE Flags: Precursor;
GN Name=Cox8b; Synonyms=Cox8h;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Heart;
RX PubMed=7711081; DOI=10.1016/0167-4781(95)00023-a;
RA Hegeman A.D., Brown J.S., Lomax M.I.;
RT "Sequence of the cDNA for the heart/muscle isoform of mouse cytochrome c
RT oxidase subunit VIII.";
RL Biochim. Biophys. Acta 1261:311-314(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules using 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P10175}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 14 subunits. The complex is composed of
CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A,
CC COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which
CC are encoded in the nuclear genome. The complex exists as a monomer or a
CC dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC III, CIII), resulting in different assemblies (supercomplex
CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P10175}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase VIII family.
CC {ECO:0000305}.
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DR EMBL; U15541; AAA80280.1; -; mRNA.
DR EMBL; BC027531; AAH27531.1; -; mRNA.
DR EMBL; BC086930; AAH86930.1; -; mRNA.
DR CCDS; CCDS21991.1; -.
DR PIR; S53706; S53706.
DR RefSeq; NP_031777.1; NM_007751.3.
DR PDB; 7O37; EM; 3.20 A; m=25-70.
DR PDB; 7O3C; EM; 3.30 A; m=25-70.
DR PDB; 7O3E; EM; 3.60 A; m=25-70.
DR PDBsum; 7O37; -.
DR PDBsum; 7O3C; -.
DR PDBsum; 7O3E; -.
DR AlphaFoldDB; P48772; -.
DR SMR; P48772; -.
DR STRING; 10090.ENSMUSP00000026561; -.
DR TCDB; 3.D.4.11.1; the proton-translocating cytochrome oxidase (cox) superfamily.
DR PaxDb; P48772; -.
DR PRIDE; P48772; -.
DR ProteomicsDB; 283610; -.
DR Antibodypedia; 182; 22 antibodies from 12 providers.
DR DNASU; 12869; -.
DR Ensembl; ENSMUST00000026561; ENSMUSP00000026561; ENSMUSG00000025488.
DR GeneID; 12869; -.
DR KEGG; mmu:12869; -.
DR UCSC; uc009kio.2; mouse.
DR CTD; 12869; -.
DR MGI; MGI:105958; Cox8b.
DR VEuPathDB; HostDB:ENSMUSG00000025488; -.
DR eggNOG; ENOG502SB3F; Eukaryota.
DR GeneTree; ENSGT00390000006255; -.
DR HOGENOM; CLU_203368_0_0_1; -.
DR InParanoid; P48772; -.
DR OMA; AGWVLYH; -.
DR OrthoDB; 1632471at2759; -.
DR PhylomeDB; P48772; -.
DR TreeFam; TF105070; -.
DR UniPathway; UPA00705; -.
DR BioGRID-ORCS; 12869; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Cox8b; mouse.
DR PRO; PR:P48772; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P48772; protein.
DR Bgee; ENSMUSG00000025488; Expressed in digastric muscle group and 138 other tissues.
DR ExpressionAtlas; P48772; baseline and differential.
DR Genevisible; P48772; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IEA:InterPro.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0045277; C:respiratory chain complex IV; IBA:GO_Central.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IEA:InterPro.
DR Gene3D; 4.10.81.10; -; 1.
DR InterPro; IPR003205; Cyt_c_oxidase_su8.
DR InterPro; IPR036548; Cyt_c_oxidase_su8_sf.
DR PANTHER; PTHR16717; PTHR16717; 1.
DR Pfam; PF02285; COX8; 1.
DR SUPFAM; SSF81431; SSF81431; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..24
FT /note="Mitochondrion"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT CHAIN 25..70
FT /note="Cytochrome c oxidase subunit 8B, mitochondrial"
FT /id="PRO_0000006178"
FT TOPO_DOM 25..35
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT TRANSMEM 36..59
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT TOPO_DOM 60..70
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT HELIX 36..51
FT /evidence="ECO:0007829|PDB:7O37"
FT HELIX 53..57
FT /evidence="ECO:0007829|PDB:7O37"
FT STRAND 61..63
FT /evidence="ECO:0007829|PDB:7O37"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:7O37"
SQ SEQUENCE 70 AA; 7332 MW; 706773BBFD7DA555 CRC64;
MPRLPPILRL LQAPAKFTVV PKAHVSAKPA KTPTSAVEQA VGISAIVVGF MVPAGWVLAH
LESYKKSSAA
