COXM1_YEAST
ID COXM1_YEAST Reviewed; 111 AA.
AC P36064; D6VX60;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=COX assembly mitochondrial protein;
DE AltName: Full=COX biogenesis factor CMC1;
DE AltName: Full=Cx9C mitochondrial COX assembly protein 1;
DE AltName: Full=Mitochondrial metallochaperone-like protein CMC1;
GN Name=CMC1; OrderedLocusNames=YKL137W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION OF FRAMESHIFTS.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18443040; DOI=10.1128/mcb.01920-07;
RA Horn D., Al-Ali H., Barrientos A.;
RT "Cmc1p is a conserved mitochondrial twin CX(9)C protein involved in
RT cytochrome c oxidase biogenesis.";
RL Mol. Cell. Biol. 28:4354-4364(2008).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=19703468; DOI=10.1016/j.jmb.2009.08.041;
RA Longen S., Bien M., Bihlmaier K., Kloeppel C., Kauff F., Hammermeister M.,
RA Westermann B., Herrmann J.M., Riemer J.;
RT "Systematic analysis of the twin cx(9)c protein family.";
RL J. Mol. Biol. 393:356-368(2009).
RN [7]
RP FUNCTION, AND INTERACTION WITH CMC2.
RX PubMed=20220131; DOI=10.1074/jbc.m110.104786;
RA Horn D., Zhou W., Trevisson E., Al-Ali H., Harris T.K., Salviati L.,
RA Barrientos A.;
RT "The conserved mitochondrial twin Cx9C protein Cmc2 Is a Cmc1 homologue
RT essential for cytochrome c oxidase biogenesis.";
RL J. Biol. Chem. 285:15088-15099(2010).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=22984289; DOI=10.1074/mcp.m112.021105;
RA Voegtle F.N., Burkhart J.M., Rao S., Gerbeth C., Hinrichs J.,
RA Martinou J.C., Chacinska A., Sickmann A., Zahedi R.P., Meisinger C.;
RT "Intermembrane space proteome of yeast mitochondria.";
RL Mol. Cell. Proteomics 11:1840-1852(2012).
CC -!- FUNCTION: Required for mitochondrial cytochrome c oxidase (COX)
CC assembly and respiration. Binds copper. May be involved in copper
CC trafficking and distribution to mitochondrial COX and SOD1.
CC {ECO:0000269|PubMed:18443040, ECO:0000269|PubMed:20220131}.
CC -!- SUBUNIT: Interacts with CMC2. {ECO:0000269|PubMed:20220131}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:18443040, ECO:0000269|PubMed:19703468}; Peripheral
CC membrane protein {ECO:0000269|PubMed:18443040,
CC ECO:0000269|PubMed:19703468}; Intermembrane side
CC {ECO:0000269|PubMed:18443040, ECO:0000269|PubMed:19703468}.
CC Mitochondrion intermembrane space {ECO:0000269|PubMed:22984289}.
CC Note=Imported into the mitochondria via the mitochondrial MIA40-ERV1
CC machinery.
CC -!- DOMAIN: The twin Cx9C motifs are involved in the recognition by the
CC mitochondrial MIA40-ERV1 disulfide relay system and the subsequent
CC transfer of disulfide bonds by dithiol/disulfide exchange reactions to
CC the newly imported protein. {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the CMC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA81978.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z28137; CAA81978.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006944; DAA09026.1; -; Genomic_DNA.
DR PIR; S37966; S37966.
DR RefSeq; NP_012785.2; NM_001179703.1.
DR AlphaFoldDB; P36064; -.
DR BioGRID; 34000; 205.
DR DIP; DIP-4543N; -.
DR IntAct; P36064; 1.
DR STRING; 4932.YKL137W; -.
DR MaxQB; P36064; -.
DR PaxDb; P36064; -.
DR PRIDE; P36064; -.
DR EnsemblFungi; YKL137W_mRNA; YKL137W; YKL137W.
DR GeneID; 853721; -.
DR KEGG; sce:YKL137W; -.
DR SGD; S000001620; CMC1.
DR VEuPathDB; FungiDB:YKL137W; -.
DR eggNOG; KOG4624; Eukaryota.
DR HOGENOM; CLU_147575_0_0_1; -.
DR InParanoid; P36064; -.
DR OMA; IKAYVEC; -.
DR BioCyc; YEAST:G3O-31914-MON; -.
DR PRO; PR:P36064; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36064; protein.
DR GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005507; F:copper ion binding; IDA:SGD.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IMP:SGD.
DR InterPro; IPR013892; Cyt_c_biogenesis_Cmc1-like.
DR PANTHER; PTHR22977; PTHR22977; 1.
DR Pfam; PF08583; Cmc1; 1.
DR PROSITE; PS51808; CHCH; 1.
PE 1: Evidence at protein level;
KW Chaperone; Copper; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat.
FT CHAIN 1..111
FT /note="COX assembly mitochondrial protein"
FT /id="PRO_0000203147"
FT DOMAIN 39..82
FT /note="CHCH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 42..52
FT /note="Cx9C motif 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT MOTIF 64..74
FT /note="Cx9C motif 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 42..74
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT DISULFID 52..64
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
SQ SEQUENCE 111 AA; 13007 MW; 8934C73F1AB75632 CRC64;
MEQNKDPQMI SKHSSRLPIW VLSPREEQQA RKNLKTETYK KCANFVQAMA DCAKANGMKV
FPTCDKQRDE MKSCLLFYQT DEKYLDGERD KIVLEKINKL EKLCQKQSST K
