ID   COXM2_SCHPO             Reviewed;          89 AA.
AC   G2TRU5;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=COX assembly mitochondrial protein 2;
DE   AltName: Full=Cx9C motif-containing protein 2;
GN   Name=cmc2; Synonyms=new24; ORFNames=SPCC4B3.20;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=21511999; DOI=10.1126/science.1203357;
RA   Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA   Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA   Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA   Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA   French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA   Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA   Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA   Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA   Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA   Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT   "Comparative functional genomics of the fission yeasts.";
RL   Science 332:930-936(2011).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=21270388; DOI=10.1534/genetics.110.123497;
RA   Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L.,
RA   Hagan I.M., Miller C.J.;
RT   "Augmented annotation of the Schizosaccharomyces pombe genome reveals
RT   additional genes required for growth and viability.";
RL   Genetics 187:1207-1217(2011).
CC   -!- FUNCTION: Required for mitochondrial cytochrome c oxidase (COX)
CC       assembly and respiration. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}; Intermembrane side
CC       {ECO:0000250}. Note=Imported into the mitochondria via the
CC       mitochondrial mia40-erv1 machinery. {ECO:0000250}.
CC   -!- DOMAIN: The twin Cx9C motifs are involved in the recognition by the
CC       mitochondrial mia40-erv1 disulfide relay system and the subsequent
CC       transfer of disulfide bonds by dithiol/disulfide exchange reactions to
CC       the newly imported protein. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CMC family. {ECO:0000305}.
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DR   EMBL; CU329672; CCD31395.1; -; Genomic_DNA.
DR   RefSeq; XP_004001748.1; XM_004001699.1.
DR   AlphaFoldDB; G2TRU5; -.
DR   SMR; G2TRU5; -.
DR   MaxQB; G2TRU5; -.
DR   PaxDb; G2TRU5; -.
DR   EnsemblFungi; SPCC4B3.20.1; SPCC4B3.20.1:pep; SPCC4B3.20.
DR   PomBase; SPCC4B3.20; cmc2.
DR   VEuPathDB; FungiDB:SPCC4B3.20; -.
DR   HOGENOM; CLU_2400937_0_0_1; -.
DR   InParanoid; G2TRU5; -.
DR   OMA; KKNCQKE; -.
DR   PRO; PR:G2TRU5; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; ISO:PomBase.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005507; F:copper ion binding; ISO:PomBase.
DR   GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; ISO:PomBase.
DR   InterPro; IPR013892; Cyt_c_biogenesis_Cmc1-like.
DR   PANTHER; PTHR22977; PTHR22977; 1.
DR   Pfam; PF08583; Cmc1; 1.
DR   PROSITE; PS51808; CHCH; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Disulfide bond; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Reference proteome; Repeat.
FT   CHAIN           1..89
FT                   /note="COX assembly mitochondrial protein 2"
FT                   /id="PRO_0000416498"
FT   DOMAIN          21..64
FT                   /note="CHCH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT   MOTIF           24..34
FT                   /note="Cx9C motif 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT   MOTIF           46..56
FT                   /note="Cx9C motif 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT   DISULFID        24..56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
FT   DISULFID        34..46
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01150"
SQ   SEQUENCE   89 AA;  10591 MW;  BA95935E966A5A8E CRC64;
     MNKSPIEEEF DLRKQHQKLL KKNCQKEIED FVKCATGRTF SVTWKCRSEN KTMKDCLTKA
     ADEISEWQIR SEYNKAKQES FIGKQDEKK