COXX_CHLAA
ID COXX_CHLAA Reviewed; 587 AA.
AC A9WAQ6;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Protoheme IX farnesyltransferase;
DE EC=2.5.1.141;
DE AltName: Full=Heme B farnesyltransferase;
DE AltName: Full=Heme O synthase;
GN Name=ctaB; OrderedLocusNames=Caur_0029;
OS Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC Chloroflexaceae; Chloroflexus.
OX NCBI_TaxID=324602;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT "Complete genome sequence of the filamentous anoxygenic phototrophic
RT bacterium Chloroflexus aurantiacus.";
RL BMC Genomics 12:334-334(2011).
CC -!- FUNCTION: Converts heme B (protoheme IX) to heme O by substitution of
CC the vinyl group on carbon 2 of heme B porphyrin ring with a
CC hydroxyethyl farnesyl side group. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate +
CC Fe(II)-heme o; Xref=Rhea:RHEA:28070, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:60344, ChEBI:CHEBI:60530,
CC ChEBI:CHEBI:175763; EC=2.5.1.141;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; heme O biosynthesis;
CC heme O from protoheme: step 1/1.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Carbon 2 of the heme B porphyrin ring is defined
CC according to the Fischer nomenclature. {ECO:0000250}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the UbiA
CC prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
CC {ECO:0000305}.
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DR EMBL; CP000909; ABY33284.1; -; Genomic_DNA.
DR RefSeq; WP_012255940.1; NC_010175.1.
DR RefSeq; YP_001633673.1; NC_010175.1.
DR AlphaFoldDB; A9WAQ6; -.
DR SMR; A9WAQ6; -.
DR STRING; 324602.Caur_0029; -.
DR EnsemblBacteria; ABY33284; ABY33284; Caur_0029.
DR KEGG; cau:Caur_0029; -.
DR PATRIC; fig|324602.8.peg.32; -.
DR eggNOG; COG0109; Bacteria.
DR HOGENOM; CLU_030009_1_0_0; -.
DR InParanoid; A9WAQ6; -.
DR OMA; WIAMSHR; -.
DR UniPathway; UPA00834; UER00712.
DR Proteomes; UP000002008; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IBA:GO_Central.
DR GO; GO:0016653; F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor; IEA:InterPro.
DR GO; GO:0008495; F:protoheme IX farnesyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006784; P:heme A biosynthetic process; IEA:InterPro.
DR GO; GO:0006783; P:heme biosynthetic process; IBA:GO_Central.
DR GO; GO:0048034; P:heme O biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13957; PT_UbiA_Cox10; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR HAMAP; MF_00154; CyoE_CtaB; 1.
DR InterPro; IPR003780; COX15/CtaA_fam.
DR InterPro; IPR006369; Protohaem_IX_farnesylTrfase.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR030470; UbiA_prenylTrfase_CS.
DR InterPro; IPR044878; UbiA_sf.
DR PANTHER; PTHR43448; PTHR43448; 1.
DR Pfam; PF02628; COX15-CtaA; 1.
DR Pfam; PF01040; UbiA; 1.
DR TIGRFAMs; TIGR01473; cyoE_ctaB; 1.
DR PROSITE; PS00943; UBIA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Heme biosynthesis; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..587
FT /note="Protoheme IX farnesyltransferase"
FT /id="PRO_0000346080"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 522..542
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 560..580
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..317
FT /note="Unknown"
FT REGION 318..587
FT /note="Protoheme IX prenyltransferase"
SQ SEQUENCE 587 AA; 62574 MW; DD4EC0B59992426E CRC64;
MAARSLMRIL LVGLGLTLAT IISGALVTIS ASATVCSSWP LCLEELVQSQ NPLVWISLLH
RLFVALALLA VFAGLVAVWR SSDTAGLPRI TAGIAAAFFL LQALAGALLV WGVPAMVADV
WHLSGALLAF GAQSLTIALI AVPTPATPER LAGRAAQMQR RLRSLAWWSA AAAGVAALAI
GARSVVPGFG MMTAVPTNSV SAVGFAALLS VWMALEARRR LRPVAETPAL ALALPRYSLF
LPALALLALL GAFIPVAGHL ISLTAIALLW AATIIAAVIL QRVPFSFATA TAGVRSQPAW
RTVVADYISL TKPKVISLLL VTTLTTMFIT EAGLPSWWLV IWTMIGGYLA AGGAGAINCA
FDSDIDINMG RTSRRPVPSG RISRRAAFIF GLILSVLSII VLWVFTTPLA AFFALLGIIY
YAWFYTGWLK RSTWQNIIIG GGAGAIPPLV GWTAVTGSVS LMAVVLFAII FYWTPPHFWA
LALVKQKDYA RAGVPMLPVV AGEAETRWQI LVYSAIMVAV SLLPVAIGAM SWIYLIGAVV
LGLRFMRDAW AVWRVGDQAA IWGLYKYSLL YLALVFAAMV ADRLIIG
