ID   COXX_HALS3              Reviewed;         459 AA.
AC   B0R3W4;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Protoheme IX farnesyltransferase;
DE            EC=2.5.1.141;
DE   AltName: Full=Heme B farnesyltransferase;
DE   AltName: Full=Heme O synthase;
GN   Name=ctaB; OrderedLocusNames=OE_1989F;
OS   Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=478009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29341 / DSM 671 / R1;
RX   PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA   Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA   Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT   "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT   R1 compared to that of strain NRC-1.";
RL   Genomics 91:335-346(2008).
CC   -!- FUNCTION: Converts heme B (protoheme IX) to heme O by substitution of
CC       the vinyl group on carbon 2 of heme B porphyrin ring with a
CC       hydroxyethyl farnesyl side group. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate +
CC         Fe(II)-heme o; Xref=Rhea:RHEA:28070, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:60344, ChEBI:CHEBI:60530,
CC         ChEBI:CHEBI:175763; EC=2.5.1.141;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; heme O biosynthesis;
CC       heme O from protoheme: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Carbon 2 of the heme B porphyrin ring is defined
CC       according to the Fischer nomenclature. {ECO:0000250}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the UbiA
CC       prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AM774415; CAP13429.1; -; Genomic_DNA.
DR   AlphaFoldDB; B0R3W4; -.
DR   SMR; B0R3W4; -.
DR   EnsemblBacteria; CAP13429; CAP13429; OE_1989F.
DR   KEGG; hsl:OE_1989F; -.
DR   HOGENOM; CLU_030009_1_1_2; -.
DR   OMA; MKPRLMW; -.
DR   PhylomeDB; B0R3W4; -.
DR   UniPathway; UPA00834; UER00712.
DR   Proteomes; UP000001321; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008495; F:protoheme IX farnesyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0048034; P:heme O biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd13957; PT_UbiA_Cox10; 1.
DR   Gene3D; 1.10.357.140; -; 1.
DR   HAMAP; MF_00154; CyoE_CtaB; 1.
DR   InterPro; IPR006369; Protohaem_IX_farnesylTrfase.
DR   InterPro; IPR000537; UbiA_prenyltransferase.
DR   InterPro; IPR044878; UbiA_sf.
DR   PANTHER; PTHR43448; PTHR43448; 1.
DR   Pfam; PF01040; UbiA; 1.
DR   TIGRFAMs; TIGR01473; cyoE_ctaB; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Heme biosynthesis; Membrane; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..459
FT                   /note="Protoheme IX farnesyltransferase"
FT                   /id="PRO_0000346098"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        262..282
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        382..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..184
FT                   /note="Unknown"
FT   REGION          185..459
FT                   /note="Protoheme IX prenyltransferase"
SQ   SEQUENCE   459 AA;  47832 MW;  DA9DADA6A4B409BB CRC64;
     MSRNTATQFV AVLAAAAMGV YSLLVLGATT SLTGAASACQ TWPSCNGQWF ALQSLDLVVV
     WGHRTAAALT GLAVVGAAVL AWRTGASRRV RTAVTLALAL YPVQVVIGAY TAMSAGAAPF
     TGVHLTLGVG IFASLVVALA WTLDAQTGDL PSAEWEGEPR HTDDGDPTQP GIVRAYVQLM
     KPRLMWLLCL VAGAGMALAS SQLGAGQQLS AATVVLTLGG GVLSIGASGT FNHVLEREQD
     EKMARTDDRP VVTDRIPPRN ALAFGVVLGV ASLAAFAAVN LLTAVLGLTA IAFYSIVYTL
     VLKPNTRQST VIGGAAGALP ALIGWVAVTG AVGVGGVVLA GVIFLWTPAH FYNLALAYKD
     DYERGGFPLM PVVEGEAKTR RHIVYYIGAT LASAVVLAEL TGLGPLYAAT TVLLGAVFLY
     FAIRLHRERD RRAAMRSFHA SNAYLGCLLV AVVLDTMVV