COXX_ROSCS
ID COXX_ROSCS Reviewed; 535 AA.
AC A7NRY4;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Protoheme IX farnesyltransferase;
DE EC=2.5.1.141;
DE AltName: Full=Heme B farnesyltransferase;
DE AltName: Full=Heme O synthase;
GN Name=ctaB; OrderedLocusNames=Rcas_4304;
OS Roseiflexus castenholzii (strain DSM 13941 / HLO8).
OC Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Roseiflexineae;
OC Roseiflexaceae; Roseiflexus.
OX NCBI_TaxID=383372;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 13941 / HLO8;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Detter J.C.,
RA Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA Mikhailova N., Bryant D.A., Hanada S., Tsukatani Y., Richardson P.;
RT "Complete sequence of Roseiflexus castenholzii DSM 13941.";
RL Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Converts heme B (protoheme IX) to heme O by substitution of
CC the vinyl group on carbon 2 of heme B porphyrin ring with a
CC hydroxyethyl farnesyl side group. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate +
CC Fe(II)-heme o; Xref=Rhea:RHEA:28070, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:60344, ChEBI:CHEBI:60530,
CC ChEBI:CHEBI:175763; EC=2.5.1.141;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; heme O biosynthesis;
CC heme O from protoheme: step 1/1.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Carbon 2 of the heme B porphyrin ring is defined
CC according to the Fischer nomenclature. {ECO:0000250}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the UbiA
CC prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
CC {ECO:0000305}.
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DR EMBL; CP000804; ABU60330.1; -; Genomic_DNA.
DR RefSeq; WP_012122751.1; NC_009767.1.
DR AlphaFoldDB; A7NRY4; -.
DR SMR; A7NRY4; -.
DR STRING; 383372.Rcas_4304; -.
DR EnsemblBacteria; ABU60330; ABU60330; Rcas_4304.
DR KEGG; rca:Rcas_4304; -.
DR eggNOG; COG0109; Bacteria.
DR eggNOG; COG1612; Bacteria.
DR HOGENOM; CLU_030009_1_1_0; -.
DR OMA; MKPRLMW; -.
DR OrthoDB; 727661at2; -.
DR UniPathway; UPA00834; UER00712.
DR Proteomes; UP000000263; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008495; F:protoheme IX farnesyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0048034; P:heme O biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13957; PT_UbiA_Cox10; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR HAMAP; MF_00154; CyoE_CtaB; 1.
DR InterPro; IPR006369; Protohaem_IX_farnesylTrfase.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR030470; UbiA_prenylTrfase_CS.
DR InterPro; IPR044878; UbiA_sf.
DR PANTHER; PTHR43448; PTHR43448; 1.
DR Pfam; PF01040; UbiA; 1.
DR TIGRFAMs; TIGR01473; cyoE_ctaB; 1.
DR PROSITE; PS00943; UBIA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Heme biosynthesis; Membrane; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..535
FT /note="Protoheme IX farnesyltransferase"
FT /id="PRO_0000346081"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 509..529
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..263
FT /note="Unknown"
FT REGION 264..535
FT /note="Protoheme IX prenyltransferase"
SQ SEQUENCE 535 AA; 57083 MW; 28159A167A34329C CRC64;
MRRENARVVA LPLFRTSWMV IALALVAYGA ILAGSIIPTM TGAAVSSIAT AVLGGALAMY
TGMRTRAAPV RLGGRATAAR RSYLTLAFAA VGMLYLAVVA GALNTSAGTL WTCQTWPGCE
ASGSGDWPAL AHRGLAGVAT ILIAALAMQT WRIRHERALR VAVACALGLM LVQNIVGLVQ
VLLAQAGESL PLAVARLTHL GLSATAWGAL VVLVTLALRR PFPSVVAAPS PATVARPGLT
DTTLLEGKPS LLKDYVSLTK PGVISLLILT TITSMYITPA GIPEWSLVLW TTIGGWLMAS
GSHSINCYLD KDIDINMGRT SRRPIPSGRI PAWHALALGV VLGMIAFAIL AIFVNMLTAL
LALAGFFYYV VIYTIWLKRT SKHNIVIGGG AGAFPPLVGW AAVTGSLAPE ALLLWLIVFF
WTPPHFWALA LIREKDYARA GVPMLPVVAG DVETRRQIVL YTLSMLALTA LPPLLGMLGW
SYLLMASIFG GLFLYYALKL RRDGTTATAW ALYKYSLLYL ALLFVAMVVD RAVFA
