COXX_ROSS1
ID COXX_ROSS1 Reviewed; 534 AA.
AC A5UPV7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Protoheme IX farnesyltransferase;
DE EC=2.5.1.141;
DE AltName: Full=Heme B farnesyltransferase;
DE AltName: Full=Heme O synthase;
GN Name=ctaB; OrderedLocusNames=RoseRS_0224;
OS Roseiflexus sp. (strain RS-1).
OC Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Roseiflexineae;
OC Roseiflexaceae; Roseiflexus.
OX NCBI_TaxID=357808;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RS-1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.;
RT "Complete sequence of Roseiflexus sp. RS-1.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Converts heme B (protoheme IX) to heme O by substitution of
CC the vinyl group on carbon 2 of heme B porphyrin ring with a
CC hydroxyethyl farnesyl side group. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate +
CC Fe(II)-heme o; Xref=Rhea:RHEA:28070, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:60344, ChEBI:CHEBI:60530,
CC ChEBI:CHEBI:175763; EC=2.5.1.141;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; heme O biosynthesis;
CC heme O from protoheme: step 1/1.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Carbon 2 of the heme B porphyrin ring is defined
CC according to the Fischer nomenclature. {ECO:0000250}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the UbiA
CC prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
CC {ECO:0000305}.
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DR EMBL; CP000686; ABQ88660.1; -; Genomic_DNA.
DR RefSeq; WP_011955019.1; NC_009523.1.
DR AlphaFoldDB; A5UPV7; -.
DR SMR; A5UPV7; -.
DR STRING; 357808.RoseRS_0224; -.
DR EnsemblBacteria; ABQ88660; ABQ88660; RoseRS_0224.
DR KEGG; rrs:RoseRS_0224; -.
DR eggNOG; COG0109; Bacteria.
DR eggNOG; COG1612; Bacteria.
DR HOGENOM; CLU_030009_1_1_0; -.
DR OMA; MKPRLMW; -.
DR OrthoDB; 727661at2; -.
DR UniPathway; UPA00834; UER00712.
DR Proteomes; UP000006554; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008495; F:protoheme IX farnesyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0048034; P:heme O biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13957; PT_UbiA_Cox10; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR HAMAP; MF_00154; CyoE_CtaB; 1.
DR InterPro; IPR006369; Protohaem_IX_farnesylTrfase.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR030470; UbiA_prenylTrfase_CS.
DR InterPro; IPR044878; UbiA_sf.
DR PANTHER; PTHR43448; PTHR43448; 1.
DR Pfam; PF01040; UbiA; 1.
DR TIGRFAMs; TIGR01473; cyoE_ctaB; 1.
DR PROSITE; PS00943; UBIA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Heme biosynthesis; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..534
FT /note="Protoheme IX farnesyltransferase"
FT /id="PRO_0000327191"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..251
FT /note="Unknown"
FT REGION 252..530
FT /note="Protoheme IX prenyltransferase"
SQ SEQUENCE 534 AA; 57006 MW; 71E4FC82B3F3F73C CRC64;
MRREHARAIL FPLVRLPWLL IVLALIAYGA VLAGGIVPAL TGAAVSGIAM FTLGAALAIY
TGMRVGAAPV RLGGRAASAR RAYLTLAFAA TGVLYLAVIF GAINTSAGTL WTCQTWPGCA
MTGDSAWLAL AHRGLAGGAT LLIAALAVQT WRIRHERSLR IAVAWALGLM LIQNLVGLAQ
VLLAQGGESQ PVAVARLMHL GLSATAWGAL VVLTTLALRR PFPAVALLRP AHVARPGMTD
TVLLEGKPSL LKDYISLTKP GVISLLILTT ITSMYITPAG IPELSLVLWT TLGGWLMASG
SHSINCYLDK DIDVNMGRTG RRPIPSGRIP AWHALALGIA LGVVAFVILA LFVNLLAAIL
ALAGFLYYVF IYTIWLKRTS KHNIVIGGGA GAFPPLVGWA AVTGSLAPEA LLLWLIVFFW
TPPHFWALAL IRQKDYARAG VPMLPVVEGD QETRRQIVIY TLSMLALTAL PPVLGMLGWA
YLMSAAVSGG LFLHYALKLR RDGTTTTAWA LYKYSLLYLA ILFVAMAVDR VVFA
