CP074_HUMAN
ID CP074_HUMAN Reviewed; 76 AA.
AC Q96GX8; R4GN89;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Uncharacterized protein C16orf74;
GN Name=C16orf74;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
RA Schaerlinger B., Frippiat J.P.;
RT "The immunoglobulin heavy chain repertoire of the urodele amphibian
RT Pleurodeles waltl: identification of a new isotype.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS] (ISOFORMS 1 AND
RP 2), AND PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-44 AND THR-46.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS](ISOFORMS 1 AND
RP 2).
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [6]
RP INTERACTION WITH PPP3CA, TISSUE SPECIFICITY (ISOFORMS 1 AND 2),
RP PHOSPHORYLATION AT THR-44, AND MUTAGENESIS OF 36-PRO--THR-41; THR-41 AND
RP THR-44.
RX PubMed=28881575; DOI=10.18632/oncotarget.10912;
RA Nakamura T., Katagiri T., Sato S., Kushibiki T., Hontani K., Tsuchikawa T.,
RA Hirano S., Nakamura Y.;
RT "Overexpression of C16orf74 is involved in aggressive pancreatic cancers.";
RL Oncotarget 8:50460-50475(2017).
CC -!- SUBUNIT: Interacts (via PxIxIT motif, when phosphorylated on Thr-44)
CC with PPP3CA. {ECO:0000269|PubMed:28881575}.
CC -!- INTERACTION:
CC Q96GX8; Q08209-2: PPP3CA; NbExp=3; IntAct=EBI-745814, EBI-11959013;
CC Q96GX8; Q13432: UNC119; NbExp=4; IntAct=EBI-745814, EBI-711260;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=V1 {ECO:0000303|PubMed:28881575};
CC IsoId=Q96GX8-1; Sequence=Displayed;
CC Name=2; Synonyms=V3 {ECO:0000303|PubMed:28881575};
CC IsoId=Q96GX8-2; Sequence=VSP_061477;
CC -!- TISSUE SPECIFICITY: [Isoform 1]: Not expressed in pancreatic duct cells
CC (at protein level). Abundantly expressed in the pancreas and weakly
CC expressed in the thyroid. {ECO:0000269|PubMed:28881575}.
CC -!- TISSUE SPECIFICITY: [Isoform 2]: Not expressed in pancreatic duct cells
CC (at protein level). Abundantly expressed in the lymph node and weakly
CC expressed in the stomach, trachea and bone marrow.
CC {ECO:0000269|PubMed:28881575}.
CC -!- MISCELLANEOUS: May act as a prognostic marker of median survival time
CC in pancreatic cancer patients. {ECO:0000269|PubMed:28881575}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BC009078; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC123908; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC018695; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AB115766; BAS02098.1; -; mRNA.
DR EMBL; BC009078; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BQ672221; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS45540.1; -. [Q96GX8-1]
DR RefSeq; NP_996850.1; NM_206967.2.
DR AlphaFoldDB; Q96GX8; -.
DR BioGRID; 135663; 8.
DR IntAct; Q96GX8; 2.
DR STRING; 9606.ENSP00000284245; -.
DR iPTMnet; Q96GX8; -.
DR PhosphoSitePlus; Q96GX8; -.
DR BioMuta; C16orf74; -.
DR EPD; Q96GX8; -.
DR jPOST; Q96GX8; -.
DR MassIVE; Q96GX8; -.
DR MaxQB; Q96GX8; -.
DR PaxDb; Q96GX8; -.
DR PeptideAtlas; Q96GX8; -.
DR PRIDE; Q96GX8; -.
DR ProteomicsDB; 76680; -.
DR Antibodypedia; 63617; 5 antibodies from 5 providers.
DR DNASU; 404550; -.
DR Ensembl; ENST00000284245.9; ENSP00000284245.3; ENSG00000154102.11. [Q96GX8-1]
DR Ensembl; ENST00000602583.5; ENSP00000473536.1; ENSG00000154102.11. [Q96GX8-2]
DR GeneID; 404550; -.
DR KEGG; hsa:404550; -.
DR MANE-Select; ENST00000284245.9; ENSP00000284245.3; NM_206967.3; NP_996850.1.
DR UCSC; uc002fjc.5; human. [Q96GX8-1]
DR CTD; 404550; -.
DR DisGeNET; 404550; -.
DR GeneCards; C16orf74; -.
DR HGNC; HGNC:23362; C16orf74.
DR HPA; ENSG00000154102; Tissue enhanced (retina).
DR neXtProt; NX_Q96GX8; -.
DR OpenTargets; ENSG00000154102; -.
DR VEuPathDB; HostDB:ENSG00000154102; -.
DR eggNOG; ENOG502SEPB; Eukaryota.
DR GeneTree; ENSGT00390000015933; -.
DR HOGENOM; CLU_172665_0_0_1; -.
DR InParanoid; Q96GX8; -.
DR OMA; CPEDGEM; -.
DR OrthoDB; 1597312at2759; -.
DR PhylomeDB; Q96GX8; -.
DR TreeFam; TF338382; -.
DR PathwayCommons; Q96GX8; -.
DR SignaLink; Q96GX8; -.
DR BioGRID-ORCS; 404550; 12 hits in 1039 CRISPR screens.
DR ChiTaRS; C16orf74; human.
DR GenomeRNAi; 404550; -.
DR Pharos; Q96GX8; Tdark.
DR PRO; PR:Q96GX8; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q96GX8; protein.
DR Bgee; ENSG00000154102; Expressed in oocyte and 115 other tissues.
DR ExpressionAtlas; Q96GX8; baseline and differential.
DR Genevisible; Q96GX8; HS.
DR InterPro; IPR027864; DUF4597.
DR PANTHER; PTHR37455; PTHR37455; 1.
DR Pfam; PF15366; DUF4597; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..76
FT /note="Uncharacterized protein C16orf74"
FT /id="PRO_0000264621"
FT REGION 36..41
FT /note="Required for interaction with PPP3CA"
FT /evidence="ECO:0000269|PubMed:28881575"
FT MOD_RES 44
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:28881575,
FT ECO:0007744|PubMed:18669648"
FT MOD_RES 46
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VAR_SEQ 1..12
FT /note="Missing (in isoform 2)"
FT /id="VSP_061477"
FT MUTAGEN 36..41
FT /note="Missing: Abolishes interaction with PPP3CA."
FT /evidence="ECO:0000269|PubMed:28881575"
FT MUTAGEN 41
FT /note="T->A: No effect on phosphorylation."
FT /evidence="ECO:0000269|PubMed:28881575"
FT MUTAGEN 44
FT /note="T->A: Abolished phosphorylation and interaction with
FT PPP3CA."
FT /evidence="ECO:0000269|PubMed:28881575"
SQ SEQUENCE 76 AA; 8118 MW; 525DD517D5EA9F12 CRC64;
MGLKMSCLKG FQMCVSSSSS SHDEAPVLND KHLDVPDIII TPPTPTGMML PRDLGSTVWL
DETGSCPDDG EIDPEA
