CP100_CHLRE
ID CP100_CHLRE Reviewed; 576 AA.
AC A8I4E9;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 57.
DE RecName: Full=Cilia- and flagella-associated protein 100 {ECO:0000305};
DE AltName: Full=Flagella-associated protein 100 {ECO:0000305|PubMed:23569216};
DE AltName: Full=Modifier of inner arms 1 protein {ECO:0000303|PubMed:23569216};
DE Short=Mia1p {ECO:0000303|PubMed:23569216};
GN Name=FAP100 {ECO:0000303|PubMed:23569216};
GN Synonyms=MIA1 {ECO:0000303|PubMed:23569216};
GN ORFNames=CHLREDRAFT_186878 {ECO:0000312|EMBL:EDP07473.1};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH FAP73, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=23569216; DOI=10.1083/jcb.201211048;
RA Yamamoto R., Song K., Yanagisawa H., Fox L., Yagi T., Wirschell M.,
RA Hirono M., Kamiya R., Nicastro D., Sale W.S.;
RT "The MIA complex is a conserved and novel dynein regulator essential for
RT normal ciliary motility.";
RL J. Cell Biol. 201:263-278(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503, and cw92;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
CC -!- FUNCTION: As part of MIA, a complex associated with the outer doublet
CC microtubules of the axoneme, may play a role in ciliary/flagellar
CC motility by regulating the assembly and the activity of axonemal inner
CC dynein arm. {ECO:0000269|PubMed:23569216}.
CC -!- SUBUNIT: Interacts with FAP73; form the modifier of inner arm (MIA)
CC complex. {ECO:0000269|PubMed:23569216}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:23569216}. Note=Localizes to the outer doublet
CC microtubules of the axoneme. {ECO:0000269|PubMed:23569216}.
CC -!- DISRUPTION PHENOTYPE: The mia1-1, mia1-2, mia1-3 and mia1-4 mutants do
CC not express the protein in the axoneme and display slightly jerky, slow
CC swimming phenotypes, reduced flagellar beat frequencies and defective
CC phototaxis. {ECO:0000269|PubMed:23569216}.
CC -!- SIMILARITY: Belongs to the CFAP100 family. {ECO:0000305}.
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DR EMBL; AB692780; BAM95825.1; -; mRNA.
DR EMBL; DS496112; EDP07473.1; -; Genomic_DNA.
DR RefSeq; XP_001699777.1; XM_001699725.1.
DR AlphaFoldDB; A8I4E9; -.
DR SMR; A8I4E9; -.
DR STRING; 3055.EDP07473; -.
DR PaxDb; A8I4E9; -.
DR PRIDE; A8I4E9; -.
DR EnsemblPlants; PNW86981; PNW86981; CHLRE_02g103950v5.
DR GeneID; 5725540; -.
DR Gramene; PNW86981; PNW86981; CHLRE_02g103950v5.
DR KEGG; cre:CHLRE_02g103950v5; -.
DR eggNOG; ENOG502QSDI; Eukaryota.
DR HOGENOM; CLU_026271_0_0_1; -.
DR InParanoid; A8I4E9; -.
DR OMA; YKIYKDF; -.
DR OrthoDB; 1092113at2759; -.
DR GO; GO:0097545; C:axonemal outer doublet; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0070840; F:dynein complex binding; IDA:UniProtKB.
DR GO; GO:0048870; P:cell motility; IMP:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR GO; GO:0036159; P:inner dynein arm assembly; IMP:UniProtKB.
DR InterPro; IPR025252; DUF4200.
DR Pfam; PF13863; DUF4200; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton; Flagellum.
FT CHAIN 1..576
FT /note="Cilia- and flagella-associated protein 100"
FT /id="PRO_0000437480"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 417..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 538..563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 125..226
FT /evidence="ECO:0000255"
FT COILED 254..311
FT /evidence="ECO:0000255"
FT COILED 342..408
FT /evidence="ECO:0000255"
FT COMPBIAS 417..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 576 AA; 65169 MW; FD5CF6AE975E6C8D CRC64;
MPIYDEASVP GTAAGRSTTD VGATAGANPF NIPADEEIFR FREEERARKE QDKLIAQTMR
VADKTTFAAQ MQATATADAR TLLRELRPPK GPKATTTLAA SSVGTLDRRK EKENMADFIA
KKREIFLLQM SLDTKRAEIK KLEERARQRE EALKKSEQML EEDALRFDAF LKENDEKVQE
AIKKAEAEAK AKQDKVLEIK RLNTATAALR SELNKYEEQL EDCRRYKEFL DSITPPEWFE
QQAAKLQRRK DALVAEWQSQ CEALKQRREA ALAAKTAAES DYANARTQQQ AERAERAIKE
SVAALKEIMK EKEPQPPNLD FEMDPEDEEM YFQEPGQLLA VYKQLEESNL FYIQNAQETE
EALEELRQKL RDTKTRMDAE AQGLQGQVST LQASIVAARE KAKRLKDRTL ENEGAFTLSM
GSSNAPTSSV TGSSGPGGPV NLKELGDKVR EVYVRCGFDA DASISTLQML TNIEMKLEEY
LNLAEGMTPD YVDGAEKARE KDRRKVARDE KLSTQHREHE ARMARALERA AAPVFKKTGK
PLMFRSAPPQ RKKVVQADDR NDEEAELEAY LAQDMI
