2SS1_ARATH
ID 2SS1_ARATH Reviewed; 164 AA.
AC P15457;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=2S seed storage protein 1;
DE AltName: Full=2S albumin storage protein;
DE AltName: Full=NWMU2-2S albumin 1;
DE Contains:
DE RecName: Full=2S seed storage protein 1 small subunit;
DE Contains:
DE RecName: Full=2S seed storage protein 1 large subunit;
DE Flags: Precursor;
GN Name=AT2S1; OrderedLocusNames=At4g27140; ORFNames=T24A18.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 38-73 AND
RP 84-162.
RC STRAIN=cv. C24;
RX PubMed=16666238; DOI=10.1104/pp.87.4.859;
RA Krebbers E., Herdies L., de Clercq A., Seurinck J., Leemans J.,
RA van Damme J., Segura M., Gheysen G., van Montagu M., Vandekerckhove J.;
RT "Determination of the processing sites of an Arabidopsis 2S albumin and
RT characterization of the complete gene family.";
RL Plant Physiol. 87:859-866(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=8012403; DOI=10.1046/j.1365-313x.1994.05040493.x;
RA da Silva Conceicao A., Krebbers E.;
RT "A cotyledon regulatory region is responsible for the different spatial
RT expression patterns of Arabidopsis 2S albumin genes.";
RL Plant J. 5:493-505(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: This is a 2S seed storage protein.
CC -!- SUBUNIT: The mature protein consists of a small and a large chain
CC linked by disulfide bonds.
CC -!- MISCELLANEOUS: This is the most abundant isoform of 2S albumin in
CC Arabidopsis.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000305}.
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DR EMBL; AH001334; AAA32743.1; -; Genomic_DNA.
DR EMBL; Z24745; CAA80870.1; -; Genomic_DNA.
DR EMBL; AL035680; CAB38844.1; -; Genomic_DNA.
DR EMBL; AL161566; CAB79569.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85306.1; -; Genomic_DNA.
DR EMBL; AF370541; AAK48968.1; -; mRNA.
DR EMBL; AY072508; AAL66923.1; -; mRNA.
DR PIR; JA0161; NWMU1.
DR RefSeq; NP_194444.1; NM_118848.2.
DR AlphaFoldDB; P15457; -.
DR SMR; P15457; -.
DR BioGRID; 14109; 3.
DR IntAct; P15457; 3.
DR STRING; 3702.AT4G27140.1; -.
DR PaxDb; P15457; -.
DR PRIDE; P15457; -.
DR ProteomicsDB; 245172; -.
DR EnsemblPlants; AT4G27140.1; AT4G27140.1; AT4G27140.
DR GeneID; 828822; -.
DR Gramene; AT4G27140.1; AT4G27140.1; AT4G27140.
DR KEGG; ath:AT4G27140; -.
DR Araport; AT4G27140; -.
DR TAIR; locus:2136477; AT4G27140.
DR eggNOG; ENOG502S7EV; Eukaryota.
DR HOGENOM; CLU_131213_1_0_1; -.
DR InParanoid; P15457; -.
DR OMA; NIMSECE; -.
DR OrthoDB; 1466684at2759; -.
DR PhylomeDB; P15457; -.
DR PRO; PR:P15457; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; P15457; baseline and differential.
DR Genevisible; P15457; AT.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00496; NAPIN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Reference proteome;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..21
FT PROPEP 22..37
FT /evidence="ECO:0000269|PubMed:16666238"
FT /id="PRO_0000032088"
FT CHAIN 38..73
FT /note="2S seed storage protein 1 small subunit"
FT /id="PRO_0000032089"
FT PROPEP 74..83
FT /evidence="ECO:0000269|PubMed:16666238"
FT /id="PRO_0000032090"
FT CHAIN 84..162
FT /note="2S seed storage protein 1 large subunit"
FT /id="PRO_0000032091"
FT PROPEP 163..164
FT /id="PRO_0000032092"
SQ SEQUENCE 164 AA; 19014 MW; 2BF28CB474D9832B CRC64;
MANKLFLVCA ALALCFLLTN ASIYRTVVEF EEDDATNPIG PKMRKCRKEF QKEQHLRACQ
QLMLQQARQG RSDEFDFEDD MENPQGQQQE QQLFQQCCNE LRQEEPDCVC PTLKQAAKAV
RLQGQHQPMQ VRKIYQTAKH LPNVCDIPQV DVCPFNIPSF PSFY
