ID   CP19A_PIG               Reviewed;         503 AA.
AC   Q29624;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Aromatase 1;
DE            EC=1.14.14.14 {ECO:0000250|UniProtKB:P11511};
DE   AltName: Full=CYPXIXA1;
DE   AltName: Full=Cytochrome P-450AROM;
DE   AltName: Full=Cytochrome P450 19 type I;
DE   AltName: Full=Estrogen synthase;
GN   Name=CYP19A1; Synonyms=CYP19;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Blastocyst;
RX   PubMed=8625924; DOI=10.1210/endo.137.4.8625924;
RA   Choi I., Simmen R.C.M., Simmen F.A.;
RT   "Molecular cloning of cytochrome P450 aromatase complementary
RT   deoxyribonucleic acid from periimplantation porcine and equine blastocysts
RT   identifies multiple novel 5'-untranslated exons expressed in embryos,
RT   endometrium, and placenta.";
RL   Endocrinology 137:1457-1467(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 342-421.
RC   TISSUE=Liver;
RX   PubMed=8129925; DOI=10.1002/mrd.1080370102;
RA   Ko Y., Choi I., Green M.L., Simmen F.A., Simmen R.C.;
RT   "Transient expression of the cytochrome P450 aromatase gene in elongating
RT   porcine blastocysts is correlated with uterine insulin-like growth factor
RT   levels during peri-implantation development.";
RL   Mol. Reprod. Dev. 37:1-11(1994).
CC   -!- FUNCTION: Catalyzes the formation of aromatic C18 estrogens from C19
CC       androgens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 O2 + 3 reduced [NADPH--hemoprotein reductase] + testosterone
CC         = 17beta-estradiol + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--
CC         hemoprotein reductase]; Xref=Rhea:RHEA:38191, Rhea:RHEA-COMP:11964,
CC         Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:16469,
CC         ChEBI:CHEBI:17347, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC         EC=1.14.14.14; Evidence={ECO:0000250|UniProtKB:P11511};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=androst-4-ene-3,17-dione + 3 O2 + 3 reduced [NADPH--
CC         hemoprotein reductase] = estrone + formate + 4 H(+) + 4 H2O + 3
CC         oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:38195,
CC         Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740,
CC         ChEBI:CHEBI:16422, ChEBI:CHEBI:17263, ChEBI:CHEBI:57618,
CC         ChEBI:CHEBI:58210; EC=1.14.14.14;
CC         Evidence={ECO:0000250|UniProtKB:P11511};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; U37311; AAC48543.1; -; mRNA.
DR   EMBL; U37312; AAC48544.1; -; mRNA.
DR   EMBL; U37309; AAC48541.1; -; mRNA.
DR   EMBL; U37310; AAC48542.1; -; mRNA.
DR   EMBL; L15471; AAA19817.1; -; Unassigned_DNA.
DR   PIR; I46570; I46570.
DR   RefSeq; NP_999594.1; NM_214429.1.
DR   AlphaFoldDB; Q29624; -.
DR   SMR; Q29624; -.
DR   PeptideAtlas; Q29624; -.
DR   PRIDE; Q29624; -.
DR   Ensembl; ENSSSCT00070009182; ENSSSCP00070007532; ENSSSCG00070004855.
DR   GeneID; 403331; -.
DR   KEGG; ssc:403331; -.
DR   CTD; 1588; -.
DR   InParanoid; Q29624; -.
DR   OrthoDB; 1247045at2759; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Chromosome 1.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IBA:GO_Central.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0008585; P:female gonad development; IBA:GO_Central.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0032355; P:response to estradiol; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..503
FT                   /note="Aromatase 1"
FT                   /id="PRO_0000051958"
FT   BINDING         437
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   503 AA;  58227 MW;  44644734885D35EC CRC64;
     MVLEMLNPMH YKVTSMVSEV VPFASIAVLL LTGFLLLVWN YKNTSSIPGP GYFLGIGPLI
     SYLRFLWMGI GSACNYYNKT YGEFIRVWIG GEETLIISKS SSVFHVMKHS HYTSRFGSKP
     GLQFIGMHEK GIIFNNNPVL WKAVRTYFMK ALSGPGLVRM VTVCADSITK HLDKLEEVRN
     DLGYVDVLTL MRRIMLDTSN NLFLGIPLDE KAIVCKIQGY FDAWQALLLK PDIFFKIPWL
     YRKYEKSVKD LKEDMEILIE KKRRRIFTAE KLEDCMDFAT ELILAEKRGE LTKENVNQCI
     LEMLIAAPDT MSVTVFFMLF LIAKHPQVEE ELMKEIQTVV GERDIRNDDM QKLEVVENFI
     YESMRYQPVV DLVMRKALED DVIDGYPVKK GTNIILNIGR MHRLEFFPKP NEFTLENFAK
     NVPYRYFQPF GFGPRACAGK YIAMVMMKVT LVILLRRFQV QTPQDRCVEK MQKKNDLSLH
     PDETSGLLEM IFIPRNSDKC FTK