ID   CP20A_MOUSE             Reviewed;         462 AA.
AC   Q8BKE6;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Cytochrome P450 20A1;
DE            EC=1.14.-.-;
GN   Name=Cyp20a1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Eye;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Heart, Kidney, Liver, Lung, Pancreas, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AK053388; BAC35373.1; -; mRNA.
DR   CCDS; CCDS14990.1; -.
DR   RefSeq; NP_084289.1; NM_030013.3.
DR   AlphaFoldDB; Q8BKE6; -.
DR   SMR; Q8BKE6; -.
DR   BioGRID; 219044; 4.
DR   STRING; 10090.ENSMUSP00000050823; -.
DR   iPTMnet; Q8BKE6; -.
DR   PhosphoSitePlus; Q8BKE6; -.
DR   EPD; Q8BKE6; -.
DR   jPOST; Q8BKE6; -.
DR   MaxQB; Q8BKE6; -.
DR   PaxDb; Q8BKE6; -.
DR   PeptideAtlas; Q8BKE6; -.
DR   PRIDE; Q8BKE6; -.
DR   ProteomicsDB; 285274; -.
DR   Antibodypedia; 34163; 280 antibodies from 30 providers.
DR   DNASU; 77951; -.
DR   Ensembl; ENSMUST00000060608; ENSMUSP00000050823; ENSMUSG00000049439.
DR   GeneID; 77951; -.
DR   KEGG; mmu:77951; -.
DR   UCSC; uc007bem.1; mouse.
DR   CTD; 57404; -.
DR   MGI; MGI:1925201; Cyp20a1.
DR   VEuPathDB; HostDB:ENSMUSG00000049439; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   GeneTree; ENSGT00500000044939; -.
DR   HOGENOM; CLU_050960_1_0_1; -.
DR   InParanoid; Q8BKE6; -.
DR   OMA; YFQVWSE; -.
DR   OrthoDB; 1038194at2759; -.
DR   PhylomeDB; Q8BKE6; -.
DR   TreeFam; TF105089; -.
DR   BioGRID-ORCS; 77951; 2 hits in 72 CRISPR screens.
DR   ChiTaRS; Cyp20a1; mouse.
DR   PRO; PR:Q8BKE6; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; Q8BKE6; protein.
DR   Bgee; ENSMUSG00000049439; Expressed in indifferent gonad and 236 other tissues.
DR   ExpressionAtlas; Q8BKE6; baseline and differential.
DR   Genevisible; Q8BKE6; MM.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   1: Evidence at protein level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..462
FT                   /note="Cytochrome P450 20A1"
FT                   /id="PRO_0000318096"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         409
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   462 AA;  52149 MW;  4ADF835E04E291F3 CRC64;
     MLDFAIFAVT FLLALVGAVL YLYPASRQAS GIPGLTPTEE KDGNLPDIVN SGSLHEFLVN
     LHERYGPVVS FWFGRRLVVS LGTTDVLKQH FNPNKTSDPF ETMLKSLLGY QSGGGSAGED
     HVRRKLYGDA VTASLHSNFP LLLQLSEELL DKWLSYPETQ HIPLSQHMLG FALKFVTRMV
     LGSTFEDEQE VIRFQKIHGT VWSEIGKGFL DGSLDKNTTR KKQYQEALMQ LESTLKKIIK
     ERKGGNFRQH TFIDSLTQGK LNEQQILEDC VVFSLASCII TARLCTWTIH FLTTTGEVQK
     KLCKEIDQVL GEGPITSEKI EQLSYCQQVL FETVRTAKLT PVSARLQDIE GKVGPFVIPK
     ETLVLYALGV VLQDPSTWPL PHRFDPDRFA DEPVMKVFSS LGFSGTWECP ELRFAYMVTA
     VLVSVLLKRL RLLAVDRQVF EMKYELVTSA REEAWITVSK RH