2SS1_BEREX
ID 2SS1_BEREX Reviewed; 146 AA.
AC P04403; P04402; Q9LRC2;
DT 20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 2.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=2S sulfur-rich seed storage protein 1;
DE AltName: Allergen=Ber e 1;
DE Contains:
DE RecName: Full=2S sulfur-rich seed storage protein small chain 1;
DE AltName: Full=2S albumin 1 small subunit;
DE Contains:
DE RecName: Full=2S sulfur-rich seed storage protein large chain 1B;
DE AltName: Full=2S albumin 1 large subunit;
DE Flags: Precursor;
GN Name=BE2S1;
OS Bertholletia excelsa (Brazil nut).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Lecythidaceae; Bertholletia.
OX NCBI_TaxID=3645;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Altenbach S.B., Pearson K.W., Leung F.W., Sun S.S.M.;
RT "Cloning and sequence analysis of a cDNA encoding a Brazil nut protein
RT exceptionally rich in methionine.";
RL Plant Mol. Biol. 8:239-250(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Bassuener R.;
RL Submitted (DEC-1990) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1840683; DOI=10.1007/bf00023994;
RA Gander E.S., Holmstroem K.O., de Paiva G.R., de Castro L.A.B., Carneiro M.,
RA Grossi de Sa M.F.;
RT "Isolation, characterization and expression of a gene coding for a 2S
RT albumin from Bertholletia excelsa (Brazil nut).";
RL Plant Mol. Biol. 16:437-448(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Yamauchi D.;
RT "Brazil nut 2S albumin was synthesized in a transgenic French bean seed
RT with a promoter of the gene for canavalin, 7S globulin from Canavalia
RT gladiata.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 37-64 AND 70-142, AND PYROGLUTAMATE FORMATION AT
RP GLN-37.
RX PubMed=3758080; DOI=10.1111/j.1432-1033.1986.tb09926.x;
RA Ampe C., van Damme J., de Castro L.A.B., Sampaio M.J.A.M., van Montagu M.,
RA Vandekerckhove J.;
RT "The amino-acid sequence of the 2S sulphur-rich proteins from seeds of
RT Brazil nut (Bertholletia excelsa H.B.K.).";
RL Eur. J. Biochem. 159:597-604(1986).
RN [6]
RP 3D-STRUCTURE MODELING, GLYCOSYLATION, AND DISULFIDE BONDS.
RX PubMed=12421566; DOI=10.1016/s0022-2836(02)01061-6;
RA Alcocer M.J., Murtagh G.J., Bailey K., Dumoulin M., Meseguer A.S.,
RA Parker M.J., Archer D.B.;
RT "The disulphide mapping, folding and characterisation of recombinant Ber e
RT 1, an allergenic protein, and SFA8, two sulphur-rich 2S plant albumins.";
RL J. Mol. Biol. 324:165-175(2002).
CC -!- FUNCTION: This is a 2S seed storage protein.
CC -!- SUBUNIT: The mature protein consists of a small and a large chain
CC linked by disulfide bonds.
CC -!- ALLERGEN: Causes an allergic reaction in human.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000305}.
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DR EMBL; M17146; AAA33010.1; -; mRNA.
DR EMBL; X57027; CAA40343.1; -; Genomic_DNA.
DR EMBL; X57028; CAA40344.1; -; Genomic_DNA.
DR EMBL; X54490; CAA38362.1; -; Genomic_DNA.
DR EMBL; AB044391; BAA96554.1; -; Genomic_DNA.
DR PIR; A25802; A25802.
DR PIR; S14946; S14946.
DR PDB; 2LVF; NMR; -; A=37-146.
DR PDBsum; 2LVF; -.
DR AlphaFoldDB; P04403; -.
DR SMR; P04403; -.
DR Allergome; 3134; Ber e 1.0101.
DR Allergome; 88; Ber e 1.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00496; NAPIN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW Pyrrolidone carboxylic acid; Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..36
FT /evidence="ECO:0000269|PubMed:3758080"
FT /id="PRO_0000032105"
FT CHAIN 37..64
FT /note="2S sulfur-rich seed storage protein small chain 1"
FT /id="PRO_0000032106"
FT PROPEP 65..69
FT /evidence="ECO:0000269|PubMed:3758080"
FT /id="PRO_0000032107"
FT CHAIN 70..142
FT /note="2S sulfur-rich seed storage protein large chain 1B"
FT /id="PRO_0000032108"
FT PROPEP 143..146
FT /id="PRO_0000032109"
FT MOD_RES 37
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:3758080"
FT DISULFID 40..92
FT /note="Interchain (between small and large chains)"
FT /evidence="ECO:0000269|PubMed:12421566"
FT DISULFID 53..81
FT /note="Interchain (between small and large chains)"
FT /evidence="ECO:0000269|PubMed:12421566"
FT DISULFID 82..130
FT /evidence="ECO:0000269|PubMed:12421566"
FT DISULFID 94..137
FT /evidence="ECO:0000269|PubMed:12421566"
FT VARIANT 91
FT /note="S -> E (in variant 1A)"
FT CONFLICT 38..39
FT /note="EE -> QQ (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 102..103
FT /note="MR -> RM (in Ref. 4; BAA96554)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="E -> K (in Ref. 4; BAA96554)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="L -> M (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 126
FT /note="I -> L (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 37..46
FT /evidence="ECO:0007829|PDB:2LVF"
FT HELIX 49..62
FT /evidence="ECO:0007829|PDB:2LVF"
FT TURN 63..66
FT /evidence="ECO:0007829|PDB:2LVF"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:2LVF"
FT HELIX 76..87
FT /evidence="ECO:0007829|PDB:2LVF"
FT HELIX 90..108
FT /evidence="ECO:0007829|PDB:2LVF"
FT HELIX 114..130
FT /evidence="ECO:0007829|PDB:2LVF"
FT TURN 138..141
FT /evidence="ECO:0007829|PDB:2LVF"
SQ SEQUENCE 146 AA; 16911 MW; A7DF778FD766410D CRC64;
MAKISVAAAA LLVLMALGHA TAFRATVTTT VVEEENQEEC REQMQRQQML SHCRMYMRQQ
MEESPYQTMP RRGMEPHMSE CCEQLEGMDE SCRCEGLRMM MMRMQQEEMQ PRGEQMRRMM
RLAENIPSRC NLSPMRCPMG GSIAGF
