ID   CP2B5_RABIT             Reviewed;         491 AA.
AC   P12789;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Cytochrome P450 2B5;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIIB5;
DE   AltName: Full=Cytochrome P450 form HP1;
DE   AltName: Full=Cytochrome P450 type B2;
GN   Name=CYP2B5;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2826996;
RA   Gasser R., Negishi M., Philpot R.M.;
RT   "Primary structures of multiple forms of cytochrome P-450 isozyme 2 derived
RT   from rabbit pulmonary and hepatic cDNAs.";
RL   Mol. Pharmacol. 33:22-30(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-56.
RX   PubMed=7654758; DOI=10.1016/0304-4165(95)00075-m;
RA   Lehnerer M., Schulze J., Petzold A., Bernhardt R., Hlavica P.;
RT   "Rabbit liver cytochrome P-450 2B5: high-level expression of the full-
RT   length protein in Escherichia coli, purification, and catalytic activity.";
RL   Biochim. Biophys. Acta 1245:107-115(1995).
CC   -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases.
CC       In liver microsomes, this enzyme is involved in an NADPH-dependent
CC       electron transport pathway. It oxidizes a variety of structurally
CC       unrelated compounds, including steroids, fatty acids, and xenobiotics.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- INDUCTION: By phenobarbital.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; M20855; AAA31223.1; -; mRNA.
DR   EMBL; S78830; AAB35177.1; -; mRNA.
DR   PIR; D27717; D27717.
DR   PIR; S31278; S31278.
DR   RefSeq; NP_001164601.1; NM_001171130.1.
DR   AlphaFoldDB; P12789; -.
DR   SMR; P12789; -.
DR   PRIDE; P12789; -.
DR   KEGG; ocu:100328947; -.
DR   InParanoid; P12789; -.
DR   Proteomes; UP000001811; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR008068; Cyt_P450_E_grp-I_CYP2B-like.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR01685; EP450ICYP2B.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase; Phosphoprotein; Reference proteome.
FT   CHAIN           1..491
FT                   /note="Cytochrome P450 2B5"
FT                   /id="PRO_0000051682"
FT   BINDING         436
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT   MOD_RES         128
FT                   /note="Phosphoserine; by PKA"
FT                   /evidence="ECO:0000250|UniProtKB:P00176"
SQ   SEQUENCE   491 AA;  55770 MW;  ABE2B978B8408DCF CRC64;
     MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPPPLPVL GNLLQMDRKG LLRSFLRLRE
     KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK IAVVDPIFQG YGVFFANGEH
     WRALRRFSLA TMRDFGMGKR SVEERIQEEA RCLVEELRKS KGALLDNTLL FHSVTSNIIC
     SIVFGKRFDY KDPVFLRLLD LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE
     INTFIGQTVE KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHRNLIL TVLTLFFAGT
     ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD AVIHEIQRLG
     DLVPFGAPHM VTKDTQFRGY VIPKNTEVFP VLSSALHDPR YFETPNTFNP GHFLDADGAL
     KRNEGFMPFS LGKRICLGEG IARTELFLFF TTILQNFSIA SPVPPEDIDL TPRESGVGNV
     PPSYQIRFLA R