CP2C3_RABIT
ID CP2C3_RABIT Reviewed; 489 AA.
AC P00182; Q29509; Q29525;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Cytochrome P450 2C3;
DE EC=1.14.14.1;
DE AltName: Full=CYPIIC3;
DE AltName: Full=Cytochrome P450 PBc3;
DE AltName: Full=Cytochrome P450 form 3B;
GN Name=CYP2C3;
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986;
RN [1]
RP PROTEIN SEQUENCE.
RX PubMed=3988762; DOI=10.1016/s0021-9258(18)89040-8;
RA Ozols J., Heinemann F.S., Johnson E.F.;
RT "The complete amino acid sequence of a constitutive form of liver
RT microsomal cytochrome P-450.";
RL J. Biol. Chem. 260:5427-5434(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6546520; DOI=10.1021/bi00297a005;
RA Leighton J.K., Debrunner-Vossbrinck B.A., Kemper B.;
RT "Isolation and sequence analysis of three cloned cDNAs for rabbit liver
RT proteins that are related to rabbit cytochrome P-450 (form 2), the major
RT phenobarbital-inducible form.";
RL Biochemistry 23:204-210(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2340269; DOI=10.1021/bi00467a021;
RA Chan G.;
RT "Structure of the rabbit cytochrome P450IIC3 gene, a constitutive member of
RT the P450IIC subfamily.";
RL Biochemistry 29:3743-3750(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=New Zealand white; TISSUE=Liver;
RA Noshiro M., Ishida H., Okuda K.;
RT "A member of cytochrome P450 2C family which is identical to rabbit
RT P450pc3.";
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases.
CC In liver microsomes, this enzyme is involved in an NADPH-dependent
CC electron transport pathway. It oxidizes a variety of structurally
CC unrelated compounds, including steroids, fatty acids, and xenobiotics.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC ChEBI:CHEBI:142491; EC=1.14.14.1;
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC membrane protein. Microsome membrane; Peripheral membrane protein.
CC -!- INDUCTION: Constitutively expressed.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; K01523; AAA31212.1; -; mRNA.
DR EMBL; M31254; AAA31175.1; -; Genomic_DNA.
DR EMBL; M31245; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31247; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31248; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31249; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31250; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31251; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31252; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; M31253; AAA31175.1; JOINED; Genomic_DNA.
DR EMBL; D26152; BAA05139.1; -; mRNA.
DR PIR; A00183; O4RBP3.
DR RefSeq; NP_001164736.1; NM_001171265.1.
DR AlphaFoldDB; P00182; -.
DR SMR; P00182; -.
DR STRING; 9986.ENSOCUP00000023608; -.
DR PRIDE; P00182; -.
DR GeneID; 100328933; -.
DR KEGG; ocu:100328933; -.
DR CTD; 100328933; -.
DR eggNOG; KOG0156; Eukaryota.
DR InParanoid; P00182; -.
DR OrthoDB; 702827at2759; -.
DR Proteomes; UP000001811; Unplaced.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane;
KW Metal-binding; Microsome; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..489
FT /note="Cytochrome P450 2C3"
FT /id="PRO_0000051694"
FT BINDING 434
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT CONFLICT 49
FT /note="D -> N (in Ref. 3; AAA31175 and 4; BAA05139)"
FT /evidence="ECO:0000305"
FT CONFLICT 82..84
FT /note="GVI -> TVK (in Ref. 3; AAA31175 and 4; BAA05139)"
FT /evidence="ECO:0000305"
FT CONFLICT 89
FT /note="D -> Y (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="I -> M (in Ref. 4; BAA05139)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="L -> LG (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 256
FT /note="S -> L (in Ref. 3; AAA31175)"
FT /evidence="ECO:0000305"
FT CONFLICT 338
FT /note="M -> S (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="T -> S (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 364
FT /note="S -> T (in Ref. 4; BAA05139)"
FT /evidence="ECO:0000305"
FT CONFLICT 429
FT /note="A -> T (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="V -> L (in Ref. 4; BAA05139)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 489 AA; 55794 MW; 860D36E42D554C75 CRC64;
MDLLIILGIC LSCVVLLSLW KKTHGKGKLP PGPTPLPVVG NLLQLETKDI NKSLSMLAKE
YGSIFTLYFG MKPAVVLYGY EGVIEALIDR GEEFSGRGIF PVFDRVTKGL GIVFSSGEKW
KETRRFSLTV LRNLGMGKKT IEERIQEEAL CLIQALRKTN ASPCDPTFLL FCVPCNVICS
VIFQNRFDYD DEKFKTLIKY FHENFELLGT PWIQLYNIFP ILHYLPGSHR QLFKNIDGQI
KFILEKVQEH QESLDSNNPR DFVDHFLIKM EKEKHKKQSE FTMDNLITTI WDVFSAGTDT
TSNTLKFALL LLLKHPEITA KVQEEIEHVI GRHRSPCMQD RTRMPYTDAV MHEIQRYVDL
VPTSLPHAVT QDIEFNGYLI PKGTDIIPSL TSVLYDDKEF PNPEKFDPGH FLDESGNFKK
SDYFMPFSAG KRACVGEGLA RMELFLLLTT ILQHFTLKPL VDPKDIDPTP VENGFVSVPP
SYELCFVPV
