ID   CP2CP_MESAU             Reviewed;         490 AA.
AC   Q08078;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Cytochrome P450 2C25;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIIC25;
DE   AltName: Full=Cytochrome P450 HSM1;
GN   Name=CYP2C25;
OS   Mesocricetus auratus (Golden hamster).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Mesocricetus.
OX   NCBI_TaxID=10036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=8114672;
RA   Sakuma T., Masaki K., Itoh S., Yokoi T., Kamataki T.;
RT   "Sex-related differences in the expression of cytochrome P450 in hamsters:
RT   cDNA cloning and examination of the expression of three distinct CYP2C
RT   cDNAs.";
RL   Mol. Pharmacol. 45:228-236(1994).
CC   -!- FUNCTION: Catalyzes the hydroxylation of tolbutamide and the N-
CC       demethylation of aminopyrine and benzphetamine. Also has testosterone
CC       hydroxylase (16 beta) activity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- INDUCTION: P450 can be induced to high levels in liver and other
CC       tissues by various foreign compounds, including drugs, pesticides, and
CC       carcinogens.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; X63022; CAA44753.1; -; mRNA.
DR   PIR; I48189; I48189.
DR   RefSeq; NP_001268615.1; NM_001281686.1.
DR   AlphaFoldDB; Q08078; -.
DR   SMR; Q08078; -.
DR   STRING; 10036.XP_005063675.1; -.
DR   GeneID; 101823450; -.
DR   KEGG; ag:CAA44753; -.
DR   eggNOG; KOG0156; Eukaryota.
DR   OrthoDB; 702827at2759; -.
DR   Proteomes; UP000189706; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..490
FT                   /note="Cytochrome P450 2C25"
FT                   /id="PRO_0000051713"
FT   BINDING         435
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   490 AA;  55967 MW;  AB040B2ECAB80453 CRC64;
     MDAVLVLVFI LSSLVFLSLW RQSSERRKLP PGPTPLPIIG NFLQIDVKNI SGSLTNFSKV
     YGPVFTLYLG MKPTVVLHGY ETVKEALIDH GEEFAGRGDF PMAERINKGL GIVFSNGNRW
     KEIRRFSLMT LRNLGMGKRN IEDRVQEEAQ CLVEELRKTN GSPCDPTFIL SCAPCNVICS
     IIFQNRFDYK DQDFLTFMKK VNENVRILSS PWLQVCNNFP SLIDYCPGSH HKITKNVNYL
     KKYILEKIEE HQESLDIENP RDFIDYYLIK LKQANHNQQS EFSLENLTTT VSDLFGAGTE
     TTSTTLRYAL LLLLKHPHVT AKVQEEIDQV VGRHRKPCMQ DRSHMPYTDA MIHEVQRFID
     LIPISLPHAV TCDIKFRDYF IPKGTTVITS LSSVLHDNKE FPNPEVFDPG HFLDKNGNFK
     KSDYFMPFSA GKRICAGEGL ARMELFLFLT TILQNFKLKS MIHPKDIDTT PVVNGFASLP
     PSYQLCFIPV