ID   2SS1_BRANA              Reviewed;         133 AA.
AC   P01091;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Napin-1;
DE   AltName: Full=1.7S seed storage protein;
DE   Contains:
DE     RecName: Full=Napin-1 small chain;
DE   Contains:
DE     RecName: Full=Napin-1 large chain;
DE   Flags: Precursor; Fragment;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Tower;
RX   PubMed=6689334;
RA   Crouch M.L., Tenbarge K.M., Simon A.E., Ferl R.;
RT   "cDNA clones for Brassica napus seed storage proteins: evidence from
RT   nucleotide sequence analysis that both subunits of napin are cleaved from a
RT   precursor polypeptide.";
RL   J. Mol. Appl. Genet. 2:273-283(1983).
CC   -!- FUNCTION: The small, basic, water-soluble napins are one of the two
CC       major kinds of storage proteins synthesized in the seed during its
CC       maturation.
CC   -!- SUBUNIT: The mature protein consists of a small and a large chain
CC       linked by disulfide bonds.
CC   -!- TISSUE SPECIFICITY: Cotyledons and the axis.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000305}.
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DR   EMBL; K01544; AAA33005.1; -; mRNA.
DR   PIR; A01330; NWRP1.
DR   AlphaFoldDB; P01091; -.
DR   SMR; P01091; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR000617; Napin/2SS/CON.
DR   PANTHER; PTHR35496; PTHR35496; 1.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PRINTS; PR00496; NAPIN.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Seed storage protein; Storage protein.
FT   CHAIN           <1..30
FT                   /note="Napin-1 small chain"
FT                   /id="PRO_0000032110"
FT   PROPEP          31..49
FT                   /id="PRO_0000032111"
FT   CHAIN           50..130
FT                   /note="Napin-1 large chain"
FT                   /id="PRO_0000032112"
FT   PROPEP          131..133
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000032113"
FT   NON_TER         1
SQ   SEQUENCE   133 AA;  15294 MW;  5FDFC7ECE3E22ACB CRC64;
     PKCRKEFQQA QHLKACQQWL HKQAMQSGGG PSWTLDGEFD FEDDMEKQGP QQRPPLHQQY
     CNELQQEEPL CVCPTLRGAS KAVKQQIQQQ EQQQGKQQMV NRIYQTATHL PKVCNIPQVS
     VCPFQKTMPG PSY