ID   CP2G1_RAT               Reviewed;         494 AA.
AC   P10610; Q64589;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 162.
DE   RecName: Full=Cytochrome P450 2G1;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIIG1;
DE   AltName: Full=Cytochrome P450 olfactive;
DE   AltName: Full=Cytochrome P450-OLF1;
GN   Name=Cyp2g1; Synonyms=Cyp2g-1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2406242; DOI=10.1016/s0021-9258(19)39886-2;
RA   Nef P., Larabee T.M., Kagimoto K., Meyer U.A.;
RT   "Olfactory-specific cytochrome P-450 (P-450olf1; IIG1). Gene structure and
RT   developmental regulation.";
RL   J. Biol. Chem. 265:2903-2907(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 2-494.
RX   PubMed=2708343; DOI=10.1016/s0021-9258(18)83497-4;
RA   Nef P., Heldman J., Lazard D., Margalit T., Jaye M., Hanukoglu I.,
RA   Lancet D.;
RT   "Olfactory-specific cytochrome P-450. cDNA cloning of a novel
RT   neuroepithelial enzyme possibly involved in chemoreception.";
RL   J. Biol. Chem. 264:6780-6785(1989).
CC   -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases.
CC       This isozyme seems to be implicated in olfaction.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- TISSUE SPECIFICITY: Olfactory epithelium.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; M31931; AAA41069.1; -; Genomic_DNA.
DR   EMBL; M31923; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31924; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31925; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31926; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31927; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31928; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31929; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M31930; AAA41069.1; JOINED; Genomic_DNA.
DR   EMBL; M33296; AAA41070.2; -; mRNA.
DR   PIR; A35551; A35551.
DR   RefSeq; NP_036919.1; NM_012787.2.
DR   AlphaFoldDB; P10610; -.
DR   SMR; P10610; -.
DR   STRING; 10116.ENSRNOP00000028261; -.
DR   PhosphoSitePlus; P10610; -.
DR   PaxDb; P10610; -.
DR   PRIDE; P10610; -.
DR   DNASU; 25251; -.
DR   Ensembl; ENSRNOT00000102573; ENSRNOP00000083307; ENSRNOG00000020827.
DR   GeneID; 25251; -.
DR   KEGG; rno:25251; -.
DR   UCSC; RGD:2477; rat.
DR   CTD; 13108; -.
DR   RGD; 2477; Cyp2g1.
DR   eggNOG; KOG0156; Eukaryota.
DR   GeneTree; ENSGT00940000161670; -.
DR   HOGENOM; CLU_001570_22_0_1; -.
DR   InParanoid; P10610; -.
DR   OMA; WPVDIFP; -.
DR   OrthoDB; 702827at2759; -.
DR   PhylomeDB; P10610; -.
DR   TreeFam; TF352043; -.
DR   PRO; PR:P10610; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000020827; Expressed in testis and 1 other tissue.
DR   Genevisible; P10610; RN.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0008392; F:arachidonic acid epoxygenase activity; IBA:GO_Central.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; TAS:RGD.
DR   GO; GO:0016712; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR   GO; GO:0008395; F:steroid hydroxylase activity; IBA:GO_Central.
DR   GO; GO:0019373; P:epoxygenase P450 pathway; IBA:GO_Central.
DR   GO; GO:0006082; P:organic acid metabolic process; IBA:GO_Central.
DR   GO; GO:0007608; P:sensory perception of smell; IEA:UniProtKB-KW.
DR   GO; GO:0006805; P:xenobiotic metabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR008067; Cyt_P450_E_grp-I_CYP2A-like.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR01684; EP450ICYP2A.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Olfaction; Oxidoreductase; Reference proteome;
KW   Sensory transduction.
FT   CHAIN           1..494
FT                   /note="Cytochrome P450 2G1"
FT                   /id="PRO_0000051765"
FT   BINDING         439
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   494 AA;  56781 MW;  8A06E256A9A48702 CRC64;
     MALGGAFSIF MTLCLSCLLI LIAWKRTSRG GKLPPGPTPI PFLGNLLQVR IDATFQSFLK
     LQKKYGSVFT VYFGPRPVVI LCGHEAVKEA LVDQADDFSG RGEMPTLEKN FQGYGLALSN
     GERWKILRRF SLTVLRNFGM GKRSIEERIQ EEAGYLLEEL HKVKGAPIDP TFYLSRTVSN
     VICSVVFGKR FDYEDQRFRS LMKMINESFV EMSMPWAQLY DMYWGVIQYF PGRHNRLYNL
     IEELKDFIAS RVKINEASFD PSNPRDFIDC FLIKMYQDKS DPHSEFNLKN LVLTTLNLFF
     AGTETVSSTL RYGFLLLMKY PEVEAKIHEE INQVIGTHRT PRVDDRAKMP YTDAVIHEIQ
     RLTDIVPLGV PHNVIRDTHF RGYFLPKGTD VYPLIGSVLK DPKYFRYPEA FYPQHFLDEQ
     GRFKKNDAFV AFSSGKRICV GEALARMELF LYFTSILQRF SLRSLVPPAD IDIAHKISGF
     GNIPPTYELC FMAR